DNASU Plasmid Repository • 480.965.5697 | Email

CDC37 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  CDC37
Gene Name:  cell division cycle 37
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH131093.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00005332

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1137nts         Open reading frame : 1 to 1137
Reference Sequence Alignment

ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
CTGGAGCGCCTGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCTGATGCTGCAGGACGCC
ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACTCTGGC
CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTGTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1185
Start on reference sequence 49
Mutations:
None
Discrepancy:
a944t,Q315L
                                

5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CDC37
Symbol Nomenclature : CDC37
SYNONYM : P50CDC37
Designation : CDC37 (cell division cycle 37, S. cerevisiae, homolog)|CDC37 cell division cycle 37 homolog|cell division cycle 37 homolog|hsp90 chaperone protein kinase-targeting subunit|hsp90 co-chaperone Cdc37
Full Nomenclature : cell division cycle 37
GENEID : 11140
GI : 60820841
GenBank Accession : AY893514
HGNC : 1735
MIM : 605065
Vega : OTTHUMG00000180578
Target GenBank: U63131

 

Reference Sequence Alignment

  

HsCD00005332      ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
NM_007065.3       ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
                  ************************************************************

HsCD00005332      CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
NM_007065.3       CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
                  ************************************************************

HsCD00005332      GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
NM_007065.3       GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
                  ************************************************************

HsCD00005332      GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
NM_007065.3       GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
                  ************************************************************

HsCD00005332      CTGGAGCGCCTGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
NM_007065.3       CTGGAGCGCCTGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
                  ************************************************************

HsCD00005332      AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
NM_007065.3       AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
                  ************************************************************

HsCD00005332      AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
NM_007065.3       AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
                  ************************************************************

HsCD00005332      GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
NM_007065.3       GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
                  ************************************************************

HsCD00005332      CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
NM_007065.3       CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
                  ************************************************************

HsCD00005332      CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
NM_007065.3       CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
                  ************************************************************

HsCD00005332      GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
NM_007065.3       GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
                  ************************************************************

HsCD00005332      GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
NM_007065.3       GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
                  ************************************************************

HsCD00005332      ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
NM_007065.3       ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
                  ************************************************************

HsCD00005332      GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
NM_007065.3       GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
                  ************************************************************

HsCD00005332      GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
NM_007065.3       GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
                  ************************************************************

HsCD00005332      CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCTGATGCTGCAGGACGCC
NM_007065.3       CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCAGATGCTGCAGGACGCC
                  *******************************************:****************

HsCD00005332      ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACTCTGGC
NM_007065.3       ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACTCTGGC
                  ************************************************************

HsCD00005332      CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
NM_007065.3       CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
                  ************************************************************

HsCD00005332      GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTGTGA
NM_007065.3       GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTGTGA
                  *********************************************************

Pathways

NCI : Integrin-linked kinase signaling
NCI : LKB1 signaling events
Reactome : Disease
Reactome : Signal Transduction
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by constitutively active EGFR
WikiPathway : TNF alpha Signaling Pathway

Clone

Cloning Information : 131093
HIP Master Clone ID : 107250
Original Clone ID : FLH131093.01X

Organism


TAX_ID : 9606
Species Specific ID: 11140

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000105401

Reagents

Labome : CDC37-antibody