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SPBP16F5.08c (Schizosaccharomyces pombe) in pSGX3 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SPBP16F5.08c
Gene Name:  flavin dependent monooxygenase (predicted)
Sequence             
Original Clone ID: 6133c1BCt10b1
Keyword: full-length cds
Species: Schizosaccharomyces pombe
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16777962
Title: Mechanism of action of a flavin-containing monooxygenase
Authors: PSI
New York SGX Research Center for Structural Genomics
SpCD00533492

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1365nts         Open reading frame : 1 to 1365

TGTCTTCCAACAATTCGAAAAATTGCAATCATTGGTGCTGGTCCCTCTGGATTAGTTACG
GCTAAAGCGCTGTTAGCAGAAAAGGCGTTTGACCAGGTCACGCTTTTTGAGCGAAGGGGG
TCTCCGGGTGGTGTTTGGAACTATACAAGTACTTTATCAAATAAGTTACCGGTTCCTAGC
ACAAATCCCATTTTAACAACCGAACCCATTGTCGGACCAGCTGCTTTACCTGTGTATCCT
TCACCTTTGTATCGTGATCTGCAAACTAATACCCCAATTGAGTTGATGGGTTATTGTGAT
CAAAGCTTTAAACCCCAAACTCTTCAATTCCCCCATCGACATACCATTCAGGAATATCAA
CGGATATATGCACAACCTCTGCTGCCCTTTATAAAATTAGCCACCGATGTCCTAGATATA
GAAAAGAAGGATGGCTCCTGGGTCGTGACATACAAAGGAACGAAAGCCGGAAGCCCGATT
AGTAAAGATATCTTTGATGCAGTCTCAATTTGTAATGGTCATTATGAAGTACCCTATATC
CCCAATATTAAAGGTCTCGATGAATACGCTAAAGCCGTGCCGGGGTCAGTTCTACACTCA
TCGCTATTCCGTGAACCAGAATTATTCGTGGGTGAATCAGTACTTGTAGTTGGTGGTGCT
TCTTCTGCAAACGATCTCGTCCGACACTTGACGCCAGTCGCTAAGCACCCTATATATCAA
AGTCTTCTTGGTGGAGGGGACATACAGAATGAATCGTTGCAACAAGTACCTGAAATTACT
AAGTTCGATCCTACCACTAGGGAAATATACTTGAAAGGTGGAAAAGTGTTATCTAATATT
GACAGAGTTATTTATTGTACCGGATACTTGTATTCCGTACCATTTCCTTCCCTGGCTAAG
TTGAAATCCCCTGAAACCAAGTTGATTGATGATGGTAGTCATGTGCACAATGTCTATCAA
CATATATTTTATATCCCCGATCCAACTCTGGCTTTTGTTGGTTTAGCTCTACACGTTGTT
CCTTTCCCTACTAGTCAAGCACAGGCAGCGTTCCTCGCGCGAGTTTGGTCTGGTCGCCTC
AAATTACCCAGCAAAGAAGAGCAGCTGAAATGGCAAGACGAATTAATGTTTTCGCTGAGT
GGAGCAAATAATATGTATCACTCTTTGGATTATCCAAAAGACGCTACCTACATTAACAAG
CTACACGACTGGTGTAAACAGGCTACACCTGTTCTTGAAGAAGAGTTTCCGTCACCGTAT
TGGGGCGAGAAGGAACGAAGCATCCGTGAAAACATGTGGTCCATCCGTGCTAAGTTTTTC
GGAATTGAACCTCCAAAGGAAGGGCATCATCACCATCACCACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-6133c
Recommended expression in: BL21 (DE3)
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: NYSGXRC
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 


Cloning Information: 461817
GI: 19112574
GenBank Accession: NM_001021682
HIP Master Clone ID: 390561
Labome: SPBP16F5-08c-cDNA
Original Clone ID: 6133c1BCt10b1
PDB - Structural Annotation: 2GVCA
Structural Annotation Wiki: NYSGXRC-6133c
Structural Biology Knowledgebase: SBKB (2GVCA)
TargetTrack - Experimental data: NYSGXRC-6133c
Species Specific ID: 2541228
Target GenBank: None
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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