| Gene: |
Gene Symbol:
SPBP16F5.08c Gene Name: flavin dependent monooxygenase (predicted) Sequence |
|---|---|
| Original Clone ID: | 6133c1BCt10b1 |
| Keyword: | full-length cds |
| Species: | Schizosaccharomyces pombe |
| Type: | cDNA |
| Vector Name: | pSGX3 Format: CLOSED |
| Source: | New York SGX Research Center for Structural Genomics |
| Description: | None |
| Comments: | None |
| Mutation/ Discrepancy : |
No / Yes |
| Publications: |
PMID: 16777962 Title: Mechanism of action of a flavin-containing monooxygenase |
| Authors: |
PSI New York SGX Research Center for Structural Genomics |
|
SpCD00533492 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI |
Insert sequence: 1365nts
Open reading frame : 1 to 1365
TGTCTTCCAACAATTCGAAAAATTGCAATCATTGGTGCTGGTCCCTCTGGATTAGTTACG
GCTAAAGCGCTGTTAGCAGAAAAGGCGTTTGACCAGGTCACGCTTTTTGAGCGAAGGGGG
TCTCCGGGTGGTGTTTGGAACTATACAAGTACTTTATCAAATAAGTTACCGGTTCCTAGC
ACAAATCCCATTTTAACAACCGAACCCATTGTCGGACCAGCTGCTTTACCTGTGTATCCT
TCACCTTTGTATCGTGATCTGCAAACTAATACCCCAATTGAGTTGATGGGTTATTGTGAT
CAAAGCTTTAAACCCCAAACTCTTCAATTCCCCCATCGACATACCATTCAGGAATATCAA
CGGATATATGCACAACCTCTGCTGCCCTTTATAAAATTAGCCACCGATGTCCTAGATATA
GAAAAGAAGGATGGCTCCTGGGTCGTGACATACAAAGGAACGAAAGCCGGAAGCCCGATT
AGTAAAGATATCTTTGATGCAGTCTCAATTTGTAATGGTCATTATGAAGTACCCTATATC
CCCAATATTAAAGGTCTCGATGAATACGCTAAAGCCGTGCCGGGGTCAGTTCTACACTCA
TCGCTATTCCGTGAACCAGAATTATTCGTGGGTGAATCAGTACTTGTAGTTGGTGGTGCT
TCTTCTGCAAACGATCTCGTCCGACACTTGACGCCAGTCGCTAAGCACCCTATATATCAA
AGTCTTCTTGGTGGAGGGGACATACAGAATGAATCGTTGCAACAAGTACCTGAAATTACT
AAGTTCGATCCTACCACTAGGGAAATATACTTGAAAGGTGGAAAAGTGTTATCTAATATT
GACAGAGTTATTTATTGTACCGGATACTTGTATTCCGTACCATTTCCTTCCCTGGCTAAG
TTGAAATCCCCTGAAACCAAGTTGATTGATGATGGTAGTCATGTGCACAATGTCTATCAA
CATATATTTTATATCCCCGATCCAACTCTGGCTTTTGTTGGTTTAGCTCTACACGTTGTT
CCTTTCCCTACTAGTCAAGCACAGGCAGCGTTCCTCGCGCGAGTTTGGTCTGGTCGCCTC
AAATTACCCAGCAAAGAAGAGCAGCTGAAATGGCAAGACGAATTAATGTTTTCGCTGAGT
GGAGCAAATAATATGTATCACTCTTTGGATTATCCAAAAGACGCTACCTACATTAACAAG
CTACACGACTGGTGTAAACAGGCTACACCTGTTCTTGAAGAAGAGTTTCCGTCACCGTAT
TGGGGCGAGAAGGAACGAAGCATCCGTGAAAACATGTGGTCCATCCGTGCTAAGTTTTTC
GGAATTGAACCTCCAAAGGAAGGGCATCATCACCATCACCACTGA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Mutations: None |
Discrepancy: None |
5' Linker Sequence:
ATGTCCCTT |
|
| 3' Linker Sequence: None | |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
kanamycin Bacterial Selection Condition: 50ug/mL kanamycin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors. |
| Protein Expression Results: |
ProteinExpressed: Protein_Confirmed ProteinPurified: Protein_Purified SolubleProtein: Protein_Soluble Find experimental protocols in NYSGXRC-6133c |
| Recommended expression in: | BL21 (DE3) |
Powered by LabGenius |
||
| Vector Name: | pSGX3 |
pSGX3 in advanced viewed |
| Synonyms: | pSGX-3, pSGX3_BC | |
| Sequencing Primer: | Forward:
T7
Reverse: T7 terminator |
|
| Description: | Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning. | |
| Comments: | Modified from pET26b from Novagen | |
| Size (bp): | 5339 | |
| Parent Vector: | None | |
| Empty Vector: | None | |
| Properties: | TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker | |
| Author Name: |
NYSGXRC |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| ATG start | start | initiation start (Met, Ser, Leu) | 5072 | 5079 |
| TOPO recognition site | TOPO | TOPO cloning site | 5076 | 5085 |
| bacterial origin | ori | pBR322-type bacterial origin of replication | 1421 | 2103 |
| gene fragment | ROP | ROP | 2527 | 2703 |
| promoter | T7 | T7 promoter | 4983 | 5003 |
| repressor protein gene | LacI | LacI CDS | 3515 | 4531 |
| selectable marker | KanR | kanamycin resistance gene | 563 | 1375 |
| tag | His | 6xHis tag | 5086 | 5103 |
| trxn regulatory element | lac operator | lac operator | 5003 | 5025 |
| trxn termination sequence | T7 term | T7 terminator | 5231 | 5307 |
| Cloning Information: |
461817 |
|---|---|
| GI: |
19112574 |
| GenBank Accession: |
NM_001021682 |
| HIP Master Clone ID: |
390561 |
| Labome: |
SPBP16F5-08c-cDNA |
| Original Clone ID: |
6133c1BCt10b1 |
| PDB - Structural Annotation: |
2GVCA |
| Structural Annotation Wiki: |
NYSGXRC-6133c |
| Structural Biology Knowledgebase: |
SBKB (2GVCA) |
| TargetTrack - Experimental data: |
NYSGXRC-6133c |
| Species Specific ID: | 2541228 |
| Target GenBank: | None |
|
PDB ID
Style
Color |
