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PPEF2 (Homo sapiens) in pSGX3 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PPEF2
Gene Name:  protein phosphatase, EF-hand calcium binding domain 2
Sequence             
Original Clone ID: 8732a4BCt1p1
Keyword: partial length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: Linker not verified.
Mutation/
Discrepancy :
Yes / Yes
Publications: None
Authors: PSI
New York SGX Research Center for Structural Genomics
HsCD00533622

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1332nts         Open reading frame : 1 to 1332
Reference Sequence Alignment

GGCAGCGGTACCTCTACGCAGCATCACTTTGCGTTCCAAAACGTCGAACGGGCANNNAAA
GCCGCGGCCCCGANNCAGCGCNGGGANCGGAGANACGNGGCACGANNAGAGATGCGTCGC
CGTTGCACTTGGAGTATCTTCCAATCAATAGAATATGCTGGGCAGCAGGATCAAGTTAAG
TTGCATGACTTTTTCTCCTACCTTATGGATCACTTTATTCCGTCGAGCCATAACGACCGC
GATTTCCTCACACGGATCTTTACCGAGGACCGTTTCGCGCAGGATTCTGAAATGAAAAAA
TGTAGTGATTATGAATCAATTGAGGTCCCAGACTCCTACACGGGACCTCGCCTGTCGTTT
CCCCTGTTACCGGATCACGCCACTGCATTGGTAGAAGCTTTTAGACTTAAGCAACAGCTG
CATGCGCGATATGTGCTCAATCTGTTATACGAGACAAAAAAACACCTGGTTCAGTTGCCA
AACATCAATCGTGTCAGCACCTGCTATTCTGAAGAAATAACGGTATGTGGCGACCTTCAT
GGTCAACTGGATGACCTCATTTTCATCTTTTATAAGAACGGGTTACCTAGTCGGGAGCGC
TCATACGTGTTCAATGGAGATTTTGTTGATCGGGGCAAAGACTCCGTCGAAATTCTGATG
ATATTGTTCGCCTTTATGCTTGTGTATCCCAAAGAGTTCCACCTGAACCGTGGTAATCAT
GAAGATCACATGGTAAACTTACGCTACCGCTTTACCAAGGAAGTTATGAATAAATATAAA
GTGCATGGTAAGGAGATCCTGCGTACTCTCCAGGACGTCTTCTGCTGGCTGCCGTTGGCA
ACACTTATTGATGAAAAAGTTCTGATCTTACATGGGGGAGTATCGGATATTACGGACCTG
GAGCTCTTGGATAAAATAGAACGCAGCAAGATCGTGTCTACCATGAGAAGTGGCACTCAA
GAAGAGTGGCGACAGGTCGTTGACATTCTGTGGTCAGATCCAATGGCTCAGGAAGGTTGT
AAAGCGAACACAATACGGGGCGGTGGGTGCTACTTTGGACCTGACGTGACCCAACAGCTT
TTACAAAAATATAATATGCAGTTTCTGATCCGTTCCCACGAATGTAAGCCGGAGGGCTAC
GAATTTTGCCATAACCGCAAAGTACTGACGATTTTTAGCGCCTCGAATTATTATGAGGTC
GGTTCTAACCGTGGGGCATACGTTAAACTCGGCCCCGCGTTGACCCCGCACATTGTGCAG
TATCAAGCTAATAAGGTAACTCATACACTTACGATGCGCCAGCGGGAAGGGCATCATCAC
CATCACCACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@320,113

Discrepancy:
t9g,G3G;t42c,N14N;
c44t,A15V;t51g,R17R;

t55n;t56n;
t57n;t63c,A21A;

t71c,L24P;t74n;
t75n;t82n;

t85g,t87n,Y29X;t94n;
t98n;t106n;

t107n;c593g,P198R;
g748c,G250R
                                

5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-8732a
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPEF2
Symbol Nomenclature : PPEF2
SYNONYM : PPP7CB
Designation : protein phosphatase 7, catalytic subunit, beta isozyme|protein phosphatase with EF hands 2|protein phosphatase, EF hand calcium-binding domain 2|serine/threonine-protein phosphatase with EF-hands 2
Full Nomenclature : protein phosphatase, EF-hand calcium binding domain 2
GENEID : 5470
GenBank Accession : NP_690910
HGNC : 9244
MIM : 602256
Vega : OTTHUMG00000160915
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00533622      ---GGCAGCGGTACCTCTACGCAGCATCACTTTGCGTTCCAAAACGTCGAACGGGCANNN
NM_006239.2       ATGGGAAGCGGCACCTCCACCCAACATCATTTTGCTTTCCAGAATGCAGAGAGAGCCTTC
                     **.***** ***** ** **.***** ***** *****.** * .**..*.**... 

HsCD00533622      AAAGCCGCGGCCCCGANNCAGCGCNGGGANCGGAGANACGNGGCACGANNAGAGATGCGT
NM_006239.2       AAGGCAGCAGCCCTGATCCAGAGATGGTACCGGCGCTACGTGGCCCGCCTGGAGATGAGG
                  **.**.**.**** **. ***.*..** * ***.*..***.***.**. ..******.* 

HsCD00533622      CGCCGTTGCACTTGGAGTATCTTCCAATCAATAGAATATGCTGGGCAGCAGGATCAAGTT
NM_006239.2       CGGCGTTGCACCTGGAGCATCTTCCAGTCTATAGAATATGCTGGGCAGCAAGACCAAGTC
                  ** ******** ***** ********.**:********************.** ***** 

HsCD00533622      AAGTTGCATGACTTTTTCTCCTACCTTATGGATCACTTTATTCCGTCGAGCCATAACGAC
NM_006239.2       AAGCTCCATGACTTCTTCAGCTATCTCATGGATCACTTCATCCCCAGCAGCCACAACGAC
                  *** * ******** ***: *** ** *********** ** ** :  ***** ******

HsCD00533622      CGCGATTTCCTCACACGGATCTTTACCGAGGACCGTTTCGCGCAGGATTCTGAAATGAAA
NM_006239.2       AGGGACTTCCTGACCCGCATATTCACTGAGGACAGATTCGCCCAGGACTCCGAGATGAAG
                  .* ** ***** **.** **.** ** ******.*:***** ***** ** **.*****.

HsCD00533622      AAATGTAGTGATTATGAATCAATTGAGGTCCCAGACTCCTACACGGGACCTCGCCTGTCG
NM_006239.2       AAATGCAGTGACTATGAATCCATAGAGGTACCCGACAGTTACACGGGGCCACGCCTCTCC
                  ***** ***** ********.**:*****.**.***:  ********.**:***** ** 

HsCD00533622      TTTCCCCTGTTACCGGATCACGCCACTGCATTGGTAGAAGCTTTTAGACTTAAGCAACAG
NM_006239.2       TTCCCACTCCTGCCTGACCATGCAACTGCCCTGGTAGAAGCATTCAGACTGAAACAACAG
                  ** **.**  *.** ** ** **.*****. **********:** ***** **.******

HsCD00533622      CTGCATGCGCGATATGTGCTCAATCTGTTATACGAGACAAAAAAACACCTGGTTCAGTTG
NM_006239.2       CTCCATGCTCGCTACGTCTTGAACCTTTTGTATGAAACCAAGAAACATCTGGTACAGCTG
                  ** ***** **.** **  * ** ** **.** **.**.**.***** *****:*** **

HsCD00533622      CCAAACATCAATCGTGTCAGCACCTGCTATTCTGAAGAAATAACGGTATGTGGCGACCTT
NM_006239.2       CCAAACATCAACCGGGTCTCAACCTGTTACAGTGAGGAGATCACAGTGTGTGGAGACTTA
                  *********** ** ***: .***** ** : ***.**.**.**.**.*****.*** *:

HsCD00533622      CATGGTCAACTGGATGACCTCATTTTCATCTTTTATAAGAACGGGTTACCTAGTCGGGAG
NM_006239.2       CATGGCCAATTGGATGACTTAATCTTTATATTTTATAAGAATGGCCTCCCGTCGCCAGAA
                  ***** *** ******** *.** ** **.*********** **  *.** :  * .**.

HsCD00533622      CGCTCATACGTGTTCAATGGAGATTTTGTTGATCGGGGCAAAGACTCCGTCGAAATTCTG
NM_006239.2       CGGTCATATGTGTTCAACGGTGACTTTGTGGATCGAGGCAAGGATTCAGTAGAGATCCTG
                  ** ***** ******** **:** ***** *****.*****.** **.**.**.** ***

HsCD00533622      ATGATATTGTTCGCCTTTATGCTTGTGTATCCCAAAGAGTTCCACCTGAACCGTGGTAAT
NM_006239.2       ATGATTCTTTTTGCCTTCATGCTGGTTTACCCCAAAGAGTTCCATCTTAACAGAGGAAAC
                  *****: * ** ***** ***** ** ** ************** ** ***.*:**:** 

HsCD00533622      CATGAAGATCACATGGTAAACTTACGCTACCGCTTTACCAAGGAAGTTATGAATAAATAT
NM_006239.2       CATGAGGACCATATGGTGAACTTACGATATGGCTTCACCAAGGAAGTGATGAATAAATAC
                  *****.** ** *****.********.**  **** *********** *********** 

HsCD00533622      AAAGTGCATGGTAAGGAGATCCTGCGTACTCTCCAGGACGTCTTCTGCTGGCTGCCGTTG
NM_006239.2       AAGGTACACGGGAAGGAAATACTAAGAACCCTGCAAGATGTTTTCTGTTGGCTTCCACTG
                  **.**.** ** *****.**.**..*:** ** **.** ** ***** ***** **. **

HsCD00533622      GCAACACTTATTGATGAAAAAGTTCTGATCTTACATGGGGGAGTATCGGATATTACGGAC
NM_006239.2       GCCACTCTGATAGATGAGAAAGTTCTAATTCTTCATGGTGGGGTGTCAGACATAACTGAT
                  **.**:** **:*****.********.**  *:***** **.**.**.** **:** ** 

HsCD00533622      CTGGAGCTCTTGGATAAAATAGAACGCAGCAAGATCGTGTCTACCATGAG----------
NM_006239.2       CTGGAGCTTTTGGACAAAATAGAGAGGAGCAAGATAGTTTCCACCATGAGGTGCAAAACG
                  ******** ***** ********..* ********.** ** ********          

HsCD00533622      ------------------------------------------------------------
NM_006239.2       AGACAGAAGAGTGAGAAGCAGATGGAGGAGAAGAGAAGAGCCAACCAGAAGAGCTCTGCA
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       CAGGGACCCATCCCATGGTTTCTCCCCGAAAGCCGCTCTCTTCCCTCTTCGCCCCTTCGG
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       CTTGGCTCCTACAAGGCCCAGAAAACCAGCAGGTCCTCCAGCATCCCCTGCAGCGGTTCC
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       CTGGACGGGCGGGAGCTCTCCCGGCAGGTGCGGAGCTCCGTGGAACTGGAGCTAGAGCGG
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       TGCCGGCAGCAAGCAGGCCTCCTGGTGACCGGAGAGAAAGAGGAGCCCTCCCGCTCAGCC
                                                                              

HsCD00533622      --------------------------------AAGTGGCACTCAAGAAGAGTGGCGACAG
NM_006239.2       TCAGAAGCAGACTCTGAAGCCGGAGAGCTGCGGAAGCCCACTCAGGAGGAGTGGAGGCAG
                                                  .*.   ******.**.******.*.***

HsCD00533622      GTCGTTGACATTCTGTGGTCAGATCCAATGGCTCAGGAAGGTTGTAAAGCGAACACAATA
NM_006239.2       GTTGTAGATATCCTGTGGAGTGATCCCATGGCTCAAGAGGGCTGCAAGGCCAACACTATT
                  ** **:** ** ******: :*****.********.**.** ** **.** *****:**:

HsCD00533622      CGGGGCGGTGGGTGCTACTTTGGACCTGACGTGACCCAACAGCTTTTACAAAAATATAAT
NM_006239.2       CGAGGAGGAGGCTGTTATTTTGGGCCTGATGTGACACAACAGTTGCTACAAAAATACAAC
                  **.**.**:** ** ** *****.***** *****.****** *  ********** ** 

HsCD00533622      ATGCAGTTTCTGATCCGTTCCCACGAATGTAAGCCGGAGGGCTACGAATTTTGCCATAAC
NM_006239.2       ATGCAATTCCTGATCCGTTCACATGAATGCAAACCTGAAGGCTATGAATTCTGTCACAAC
                  *****.** ***********.** ***** **.** **.***** ***** ** ** ***

HsCD00533622      CGCAAAGTACTGACGATTTTTAGCGCCTCGAATTATTATGAGGTCGGTTCTAACCGTGGG
NM_006239.2       CGCAAGGTATTAACAATCTTTTCTGCCTCCAACTACTATGAAGTTGGCAGCAACAGAGGG
                  *****.*** *.**.** ***:  ***** ** ** *****.** ** :  ***.*:***

HsCD00533622      GCATACGTTAAACTCGGCCCCGCGTTGACCCCGCACATTGTGCAGTATCAAGCTAATAAG
NM_006239.2       GCCTATGTCAAACTGGGGCCAGCCCTGACCCCACATATTGTGCAGTATCAAGCTAACAAG
                  **.** ** ***** ** **.**  *******.** ******************** ***

HsCD00533622      GTAACTCATACACTTACGATGCGCCAGCGGGAAG----------GGCATCATCACCATCA
NM_006239.2       GTGACCCACACACTCACCATGAGGCAAAGGATTAGCAGAGTGGAGGAGTCGGCTCTGAGA
                  **.** ** ***** ** ***.* **..**.::.          **..**. *:* .: *

HsCD00533622      CCACTGA-----------------------------------------------------
NM_006239.2       GCTCTGAGGGAGAAGCTGTTTGCTCATTCTTCAGATCTTCTCAGTGAATTTAAGAAGCAT
                   *:****                                                     

HsCD00533622      ------------------------------------------------------------
NM_006239.2       GATGCAGATAAAGTCGGTTTAATCACCTTGAGTGACTGGGCAGCAGCGGTGGAGTCTGTG
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       TTGCACCTAGGACTGCCATGGCGGATGCTGAGGCCACAGCTGGTGAACAGCTCAGCAGAC
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       AACATGCTGGAGTACAAGTCTTGGCTGAAGAACTTGGCCAAGGAACAACTGAGTCGCGAG
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       AACATACAATCAAGTTTGCTGGAAACATTGTATCGAAACCGATCCAACCTAGAGACCATT
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       TTTAGGATCATAGACAGTGATCATTCAGGGTTCATCTCACTGGACGAGTTCAGGCAGACC
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       TGGAAGCTGTTCAGCTCTCACATGAATATCGACATTACAGATGACTGCATCTGTGACCTT
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       GCTCGGAGCATTGATTTCAACAAAGATGGCCACATTGATATCAATGAGTTCCTGGAGGCC
                                                                              

HsCD00533622      ------------------------------------------------------------
NM_006239.2       TTCCGCCTTGTGGAGAAATCCTGCCCAGAGGGCGATGCCTCAGAATGCCCACAAGCTACA
                                                                              

HsCD00533622      ------------------------------------------
NM_006239.2       AATGCTAAAGACAGTGGCTGCAGCAGTCCAGGTGCACACTAA
                                                            

Protein

SMART domain : PP2Ac : Protein phosphatase 2A homologues, catalytic domain.
Structural Annotation Wiki : NYSGXRC-8732a
HPRD : 03771

Clone

Cloning Information : 475874
HIP Master Clone ID : 397380
Original Clone ID : 8732a4BCt1p1

Experimental

TargetTrack - Experimental data : NYSGXRC-8732a

Organism


TAX_ID : 9606
Species Specific ID: None

Genome

Chromosome : 4
Map Location : 4q21.1
Ensembl : ENSG00000156194

Reagents

Labome : PPEF2-antibody