PPEF2 (H. sapiens )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PPEF2
Gene Name:  protein phosphatase, EF-hand calcium binding domain 2
Original Clone ID:
8732a4BCt1p1
Keyword:
partial length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
Yes / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
HsCD00533622
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1332nts
Open reading frame: 1 to 1332

GGCAGCGGTACCTCTACGCAGCATCACTTTGCGTTCCAAAACGTCGAACGGGCANNNAAA
GCCGCGGCCCCGANNCAGCGCNGGGANCGGAGANACGNGGCACGANNAGAGATGCGTCGC
CGTTGCACTTGGAGTATCTTCCAATCAATAGAATATGCTGGGCAGCAGGATCAAGTTAAG
TTGCATGACTTTTTCTCCTACCTTATGGATCACTTTATTCCGTCGAGCCATAACGACCGC
GATTTCCTCACACGGATCTTTACCGAGGACCGTTTCGCGCAGGATTCTGAAATGAAAAAA
TGTAGTGATTATGAATCAATTGAGGTCCCAGACTCCTACACGGGACCTCGCCTGTCGTTT
CCCCTGTTACCGGATCACGCCACTGCATTGGTAGAAGCTTTTAGACTTAAGCAACAGCTG
CATGCGCGATATGTGCTCAATCTGTTATACGAGACAAAAAAACACCTGGTTCAGTTGCCA
AACATCAATCGTGTCAGCACCTGCTATTCTGAAGAAATAACGGTATGTGGCGACCTTCAT
GGTCAACTGGATGACCTCATTTTCATCTTTTATAAGAACGGGTTACCTAGTCGGGAGCGC
TCATACGTGTTCAATGGAGATTTTGTTGATCGGGGCAAAGACTCCGTCGAAATTCTGATG
ATATTGTTCGCCTTTATGCTTGTGTATCCCAAAGAGTTCCACCTGAACCGTGGTAATCAT
GAAGATCACATGGTAAACTTACGCTACCGCTTTACCAAGGAAGTTATGAATAAATATAAA
GTGCATGGTAAGGAGATCCTGCGTACTCTCCAGGACGTCTTCTGCTGGCTGCCGTTGGCA
ACACTTATTGATGAAAAAGTTCTGATCTTACATGGGGGAGTATCGGATATTACGGACCTG
GAGCTCTTGGATAAAATAGAACGCAGCAAGATCGTGTCTACCATGAGAAGTGGCACTCAA
GAAGAGTGGCGACAGGTCGTTGACATTCTGTGGTCAGATCCAATGGCTCAGGAAGGTTGT
AAAGCGAACACAATACGGGGCGGTGGGTGCTACTTTGGACCTGACGTGACCCAACAGCTT
TTACAAAAATATAATATGCAGTTTCTGATCCGTTCCCACGAATGTAAGCCGGAGGGCTAC
GAATTTTGCCATAACCGCAAAGTACTGACGATTTTTAGCGCCTCGAATTATTATGAGGTC
GGTTCTAACCGTGGGGCATACGTTAAACTCGGCCCCGCGTTGACCCCGCACATTGTGCAG
TATCAAGCTAATAAGGTAACTCATACACTTACGATGCGCCAGCGGGAAGGGCATCATCAC
CATCACCACTGA

Protein sequence predicted based on nucleotide sequence

GSGTSTQHHFAFQNVERAKAAAPQRRRAREMRRRCTWSIFQSIEYAGQQDQVKLHDFFSY
LMDHFIPSSHNDRDFLTRIFTEDRFAQDSEMKKCSDYESIEVPDSYTGPRLSFPLLPDHA
TALVEAFRLKQQLHARYVLNLLYETKKHLVQLPNINRVSTCYSEEITVCGDLHGQLDDLI
FIFYKNGLPSRERSYVFNGDFVDRGKDSVEILMILFAFMLVYPKEFHLNRGNHEDHMVNL
RYRFTKEVMNKYKVHGKEILRTLQDVFCWLPLATLIDEKVLILHGGVSDITDLELLDKIE
RSKIVSTMRSGTQEEWRQVVDILWSDPMAQEGCKANTIRGGGCYFGPDVTQQLLQKYNMQ
FLIRSHECKPEGYEFCHNRKVLTIFSASNYYEVGSNRGAYVKLGPALTPHIVQYQANKVT
HTLTMRQREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@320,113

Discrepancy:
t9g,G3G;t42c,N14N;
c44t,A15V;t51g,R17R;

t55n;t56n;
t57n;t63c,A21A;

t71c,L24P;t74n;
t75n;t82n;

t85g,t87n,Y29X;t94n;
t98n;t106n;

t107n;c593g,P198R;
g748c,G250R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-8732a
Recommended expression in:
Not Applicable