Detailed Vector Information: pGEn3-DEST

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pGEn3-DEST
Source:
Kelley Moremen
Description:
Mammalian expression vector with a CMV promoter and N-terminal signal sequence, AviTag, superfolder GFP and Fc fragment; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments:
Empty vector contains ccdB death cassette, which is removed in constructs containing a gene insert
Publications:
None
Authors:
University of Georgia
Kelley Moremen
EvNO00534625
Price: 
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UNAVAILABLE
No restriction
Special MTA: SPTA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.