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Detailed Vector Information: pGEn3-DEST

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pGEn3-DEST              
Source: Kelley Moremen
Description: Mammalian expression vector with a CMV promoter and N-terminal signal sequence, AviTag, superfolder GFP and Fc fragment; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: Empty vector contains ccdB death cassette, which is removed in constructs containing a gene insert
Publications: None
Authors: University of Georgia
Kelley Moremen
EvNO00534625

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AVAILABLE
No restriction
Special MTA: SPTA

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pGEn3-DEST

pGEn3-DEST in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pGEn3-DEST-5'F
Reverse:  pGEn1-DEST R
Description: Mammalian expression vector with a CMV promoter and N-terminal signal sequence, AviTag, superfolder GFP and Fc fragment; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: None
Size (bp): 7632
Parent Vector: Name: pXKM
Description:
Properties: Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: University of Georgia
Kelley Moremen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 1137 1804
death cassette ccdB ccdB death cassette (removed in vectors containing an insert) 5774 6079
gene fragment Fc region fragment crystallizable region (Fc region) 3832 4527
intron artificial intron artificial intron 2828 2960
poly-A signal BGH pA bovine growth hormone (BGH) polyadenylation signal 6808 7022
promoter CMV CMV promoter 2208 2790
recombination site 3'-ITR inverted terminal repeat sequence 7110 7239
recombination site attR2 attR2 recombinational cloning site 6227 6242
recombination site 5'ITR inverted terminal repeat sequence 1998 2127
recombination site attR1 attR1 recombinational cloning site 4540 4556
selectable marker AmpR Ampicillin resistance 129 1108
selectable marker CmR Chloramphenicol resistance (removed in vectors containing an insert) 4773 5429
signal sequence TCM TCM signal sequence 2977 3051
tag AviTag N-terminal AviTag 3079 3129
tag His N-terminal 10xHis tag 3055 3078
tag GFP N-terminal superfolder GFP 3130 3825
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 6260 6801

 

Species Specific ID: None
Target GenBank: None