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Detailed Vector Information: pGEc1-DEST

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pGEc1-DEST              
Source: Kelley Moremen
Description: Mammalian expression vector with a CMV promoter and C-terminal 8xHis and Strep tags; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: Empty vector contains ccdB death cassette, which is removed in constructs containing a gene insert
Publications: None
Authors: University of Georgia
Kelley Moremen
EvNO00534626

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AVAILABLE
No restriction
Special MTA: SPTA

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pGEc1-DEST

pGEc1-DEST in advanced viewed

Synonyms: pXLG-CtermHisStrep-DEST
Sequencing Primer: Forward:  pGEc1-DEST F
Reverse:  pGEn1-DEST R
Description: Mammalian expression vector with a CMV promoter and C-terminal 8xHis and Strep tags; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: http://glycoenzymes.ccrc.uga.edu/
Size (bp): 6126
Parent Vector: None
Properties: Gateway, acceptor (destination), mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: University of Georgia
Kelley Moremen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE Col E1 bacterial origin 1137 1804
death cassette ccdB ccdB death cassette (removed in plasmids with insert) 4211 4516
intron artificial intron artificial intron 2828 2960
poly-A signal BGH pA bovine growth hormone (BGH) polyadenylation signal 5302 5516
promoter CMV CMV promoter 2208 2790
recombination site 5'ITR inverted terminal repeat sequence 1998 2127
recombination site attR2 attR2 site 4557 4681
recombination site attR1 attR1 site 2974 3101
recombination site 3'-ITR inverted terminal repeat sequence 5604 5733
selectable marker CmR Chloramphenicol resistance (CmR) gene (removed in plasmids with insert) 3210 2859
selectable marker AmpR ampicillin resistance gene 129 989
tag His C-terminal 8xHis tag 4691 4714
tag strep strep tag 4718 4741
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 4754 5295

 

Species Specific ID: None
Target GenBank: None