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Explanation of Terms


Gene: Gene Symbol:  CA8
Gene Name:  carbonic anhydrase VIII
Sequence             
Original Clone ID: HR3028-21.4
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pET21_NESG               Format:  FUSION
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Northeast Structural Genomics Consortium
HsCD00536530

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 870nts         Open reading frame : 1 to 870
Reference Sequence Alignment

ATGGCGGACCTGAGCTTCATCGAAGATACCGTCGCCTTCCCCGAGAAGGAAGAGGATGAG
GAGGAAGAAGAGGAGGGTGTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGGGTCTGGTG
TTTCCTGATGCTAATGGGGAATACCAGTCTCCTATTAACCTAAACTCAAGAGAGGCTAGG
TATGACCCCTCGCTGCTGGATGTCCGCCTCTCCCCAAATTATGTGGTGTGCCGAGACTGT
GAAGTCACCAATGATGGACATACCATTCAGGTTATCCTGAAGTCAAAATCAGTTCTTTCG
GGAGGACCATTGCCTCAAGGGCATGAGTTTGAACTGTACGAAGTGAGATTTCACTGGGGA
AGAGAAAACCAGCGTGGTTCTGAGCACACGGTTAATTTCAAAGCTTTTCCCATGGAGCTC
CATCTGATCCACTGGAACTCCACTCTGTTTGGCAGCATTGATGAGGCTGTGGGGAAGCCG
CACGGAATCGCCATCATTGCTCTGTTTGTTCAGATAGGAAAGGAACATGTTGGCTTGAAG
GCTGTGACTGAAATCCTCCAAGATATTCAGTATAAGGGGAAGTCCAAAACAATACCTTGC
TTTAATCCTAACACTTTATTACCAGACCCTCTGCTGCGGGATTACTGGGTGTATGAAGGC
TCTCTCACCATCCCACCTTGCAGTGAAGGTGTCACCTGGATATTATTCCGATACCCTTTA
ACTATATCCCAGCTACAGATAGAAGAATTTCGAAGGCTGAGGACACATGTTAAGGGGGCA
GAACTTGTGGAAGGCTGTGATGGGATTTTGGGAGACAACTTTCGGCCCACTCAGCCTCTT
AGTGACAGAGTCATTAGAGCTGCATTTCAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAGGAGATATACAT

3' Linker Sequence:
CTCGAGCACCACCACCACCACCACTGA

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
Find experimental protocols in HR3028
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET21_NESG

pET21_NESG in advanced viewed

Synonyms: pET21, pET21-modified, pET21Cc, pET21_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5415
Parent Vector: None
Empty Vector: EvNO00336944
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Thomas B. Acton
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, BamHI, EcoRI, XhoI) 157 253
bacterial origin ori pBR322-type bacterial origin of replication 3180 3862
gene fragment ROP ROP 2580 2756
primer T7 forward T7 forward sequencing primer 283 298
primer T7 reverse T7 reverse sequencing primer 66 84
promoter T7 T7 promoter 280 299
repressor binding site lacO lacO regulatory sequence 258 280
repressor protein gene LacI LacI 752 1768
selectable marker AmpR ampicillin resistance gene 3960 4619
ssDNA origin f1 ori f1 origin 5081 5387
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 termination sequence 68 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome

Gene

 

Gene Symbol : CA8
Symbol Nomenclature : CA8
SYNONYM : CA-VIII; CALS; CAMRQ3; CARP
Designation : CA-related protein|carbonate dehydratase|carbonic anhydrase-like sequence|carbonic anhydrase-related protein
Full Nomenclature : carbonic anhydrase VIII
GENEID : 767
HGNC : 1382
MIM : 114815
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00536530      ATGGCGGACCTGAGCTTCATCGAAGATACCGTCGCCTTCCCCGAGAAGGAAGAGGATGAG
NM_004056.4       ATGGCGGACCTGAGCTTCATCGAAGATACCGTCGCCTTCCCCGAGAAGGAAGAGGATGAG
                  ************************************************************

HsCD00536530      GAGGAAGAAGAGGAGGGTGTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGGGTCTGGTG
NM_004056.4       GAGGAAGAAGAGGAGGGTGTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGGGTCTGGTG
                  ************************************************************

HsCD00536530      TTTCCTGATGCTAATGGGGAATACCAGTCTCCTATTAACCTAAACTCAAGAGAGGCTAGG
NM_004056.4       TTTCCTGATGCTAATGGGGAATACCAGTCTCCTATTAACCTAAACTCAAGAGAGGCTAGG
                  ************************************************************

HsCD00536530      TATGACCCCTCGCTGCTGGATGTCCGCCTCTCCCCAAATTATGTGGTGTGCCGAGACTGT
NM_004056.4       TATGACCCCTCGCTGTTGGATGTCCGCCTCTCCCCAAATTATGTGGTGTGCCGAGACTGT
                  *************** ********************************************

HsCD00536530      GAAGTCACCAATGATGGACATACCATTCAGGTTATCCTGAAGTCAAAATCAGTTCTTTCG
NM_004056.4       GAAGTCACCAATGATGGACATACCATTCAGGTTATCCTGAAGTCAAAATCAGTTCTTTCG
                  ************************************************************

HsCD00536530      GGAGGACCATTGCCTCAAGGGCATGAGTTTGAACTGTACGAAGTGAGATTTCACTGGGGA
NM_004056.4       GGAGGACCATTGCCTCAAGGGCATGAATTTGAACTGTACGAAGTGAGATTTCACTGGGGA
                  **************************.*********************************

HsCD00536530      AGAGAAAACCAGCGTGGTTCTGAGCACACGGTTAATTTCAAAGCTTTTCCCATGGAGCTC
NM_004056.4       AGAGAAAACCAGCGTGGTTCTGAGCACACGGTTAATTTCAAAGCTTTTCCCATGGAGCTC
                  ************************************************************

HsCD00536530      CATCTGATCCACTGGAACTCCACTCTGTTTGGCAGCATTGATGAGGCTGTGGGGAAGCCG
NM_004056.4       CATCTGATCCACTGGAACTCCACTCTGTTTGGCAGCATTGATGAGGCTGTGGGGAAGCCG
                  ************************************************************

HsCD00536530      CACGGAATCGCCATCATTGCTCTGTTTGTTCAGATAGGAAAGGAACATGTTGGCTTGAAG
NM_004056.4       CACGGAATCGCCATCATTGCTCTGTTTGTTCAGATAGGAAAGGAACATGTTGGCTTGAAG
                  ************************************************************

HsCD00536530      GCTGTGACTGAAATCCTCCAAGATATTCAGTATAAGGGGAAGTCCAAAACAATACCTTGC
NM_004056.4       GCTGTGACTGAAATCCTCCAAGATATTCAGTATAAGGGGAAGTCCAAAACAATACCTTGC
                  ************************************************************

HsCD00536530      TTTAATCCTAACACTTTATTACCAGACCCTCTGCTGCGGGATTACTGGGTGTATGAAGGC
NM_004056.4       TTTAATCCTAACACTTTATTACCAGACCCTCTGCTGCGGGATTACTGGGTGTATGAAGGC
                  ************************************************************

HsCD00536530      TCTCTCACCATCCCACCTTGCAGTGAAGGTGTCACCTGGATATTATTCCGATACCCTTTA
NM_004056.4       TCTCTCACCATCCCACCTTGCAGTGAAGGTGTCACCTGGATATTATTCCGATACCCTTTA
                  ************************************************************

HsCD00536530      ACTATATCCCAGCTACAGATAGAAGAATTTCGAAGGCTGAGGACACATGTTAAGGGGGCA
NM_004056.4       ACTATATCCCAGCTACAGATAGAAGAATTTCGAAGGCTGAGGACACATGTTAAGGGGGCA
                  ************************************************************

HsCD00536530      GAACTTGTGGAAGGCTGTGATGGGATTTTGGGAGACAACTTTCGGCCCACTCAGCCTCTT
NM_004056.4       GAACTTGTGGAAGGCTGTGATGGGATTTTGGGAGACAACTTTCGGCCCACTCAGCCTCTT
                  ************************************************************

HsCD00536530      AGTGACAGAGTCATTAGAGCTGCATTTCAG---
NM_004056.4       AGTGACAGAGTCATTAGAGCTGCATTTCAGTAG
                  ******************************   

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
Structural Annotation Wiki : HR3028
UniProt : CAH8_HUMAN
HPRD : 00266

Clone

Cloning Information : 542980
HIP Master Clone ID : 453300
Original Clone ID : HR3028-21.4

Experimental

TargetTrack - Experimental data : HR3028

Organism


TAX_ID : 9606
Species Specific ID: None

Genome

Chromosome : 8
Map Location : 8q12.1