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NCOA2 (Homo sapiens) in pET15_NESG (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NCOA2
Gene Name:  nuclear receptor coactivator 2
Sequence             
Original Clone ID: HR3538F-27-182-14.7
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pET15_NESG               Format:  CLOSED
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Northeast Structural Genomics Consortium
Rutgers University
HsCD00539728

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 474nts         Open reading frame : 51 to 524
Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGGCCCGA
GCCCGAAACGTAATACCGAAAAACGTAACCGTGAGCAGGAAAATAAATATATTGAAGAAC
TGGCGGAACTGATTTTTGCGAACTTTAACGATATTGATAACTTTAACTTTAAACCGGATA
AATGCGCGATTCTGAAAGAAACCGTGAAGCAGATTCGTCAGATTAAAGAGCAGGAAAAAG
CAGCGGCGGCGAATATTGATGAAGTGCAGAAAAGCGATGTGAGCAGCACCGGCCAGGGCG
TGATTGATAAAGATGCGCTGGGCCCGATGATGCTGGAAGCGCTGGATGGCTTTTTTTTTG
TGGTGAACCTGGAAGGCAACGTGGTGTTTGTGAGCGAAAACGTGACCCAGTATCTGCGTT
ATAACCAGGAAGAACTGATGAATAAAAGCGTGTATAGCATTCTGCATGTGGGCGATCATA
CCGAATTTGTGAAAAACCTGCTGCCGAAAAGCATTGTGAACTAGCTCGAGATCCGGCTGC
TAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
g192a,A64A
                                

5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT

3' Linker Sequence:
CTCGAGATCCGGCTGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
Find experimental protocols in HR3538F
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NCOA2
Symbol Nomenclature : NCOA2
SYNONYM : GRIP1; KAT13C; NCoA-2; SRC2; TIF2; bHLHe75
Designation : class E basic helix-loop-helix protein 75|glucocorticoid receptor-interacting protein-1|hTIF2|transcriptional intermediary factor 2
Full Nomenclature : nuclear receptor coactivator 2
GENEID : 10499
GI : 5729858
GenBank Accession : NM_006540
HGNC : 7669
MIM : 601993
Vega : OTTHUMG00000164671
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00539728      ---CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAG-----------
NM_006540.2       ATGAGTGGGATGGGAGAAAATACCTCTGACCCCTCCAGGGCAGAGACAAGAAAGCGCAAG
                     . * ...:.*****:*::.*.*  *.*. *:***  .*..: ***.           

HsCD00539728      -----------CCATATGGGCCCGAGCCCGAAACGTAATACCGAAAAACGTAACCGTGAG
NM_006540.2       GAATGTCCTGACCAACTTGGACCCAGCCCCAAAAGGAACACTGAAAAACGTAATCGTGAA
                             ***:.* **.** ***** ***.* ** ** *********** *****.

HsCD00539728      CAGGAAAATAAATATATTGAAGAACTGGCGGAACTGATTTTTGCGAACTTTAACGATATT
NM_006540.2       CAGGAAAATAAATATATAGAAGAACTTGCAGAGTTGATTTTTGCAAATTTTAATGATATA
                  *****************:******** **.**. **********.** ***** *****:

HsCD00539728      GATAACTTTAACTTTAAACCGGATAAATGCGCGATTCTGAAAGAAACCGTGAAGCAGATT
NM_006540.2       GACAACTTTAACTTCAAACCTGACAAATGTGCAATCTTAAAAGAAACTGTGAAGCAAATT
                  ** *********** ***** ** ***** **.**  *.******** ********.***

HsCD00539728      CGTCAGATTAAAGAGCAGGAAAAAGCAGCGGCGGCGAATATTGATGAAGTGCAGAAAAGC
NM_006540.2       CGTCAGATCAAAGAACAAGAGAAAGCAGCAGCTGCCAACATAGATGAAGTGCAGAAGTCA
                  ******** *****.**.**.********.** ** ** **:**************.: .

HsCD00539728      GATGTGAGCAGCACCGGCCAGGGCGTGATTGATAAAGATGCGCTGGGCCCGATGATGCTG
NM_006540.2       GATGTATCCTCTACAGGGCAGGGTGTCATCGACAAGGATGCGCTGGGGCCTATGATGCTT
                  *****.: *:  **.** ***** ** ** ** **.*********** ** ******** 

HsCD00539728      GAAGCGCTGGATGGCTTTTTTTTTGTGGTGAACCTGGAAGGCAACGTGGTGTTTGTGAGC
NM_006540.2       GAGGCCCTTGATGGGTTCTTCTTTGTAGTGAACCTGGAAGGCAACGTTGTGTTTGTGTCA
                  **.** ** ***** ** ** *****.******************** *********: .

HsCD00539728      GAAAACGTGACCCAGTATCTGCGTTATAACCAGGAAGAACTGATGAATAAAAGCGTGTAT
NM_006540.2       GAGAATGTGACACAGTATCTAAGGTATAACCAAGAAGAGCTGATGAACAAAAGTGTATAT
                  **.** *****.********..* ********.*****.******** ***** **.***

HsCD00539728      AGCATTCTGCATGTGGGCGATCATACCGAATTTGTGAAAAACCTGCTGCCGAAAAGCATT
NM_006540.2       AGCATCTTGCATGTTGGGGACCACACGGAATTTGTCAAAAACCTGCTGCCAAAGTCTATA
                  *****  ******* ** ** ** ** ******** **************.**.:  **:

HsCD00539728      GTGAACTAG------------------------------------------CTCGAGATC
NM_006540.2       GTAAATGGGGGATCTTGGTCTGGCGAACCTCCGAGGCGGAACAGCCATACCTTCAATTGT
                  **.**  .*                                           **.* :  

HsCD00539728      CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG------------------
NM_006540.2       CGGATGCTGGTAAAACCTTTACCTGATTCAGAAGAGGAGGGTCATGATAACCAGGAAGCT
                  ***.****.. ***.**  :*.  .*   ...: .* :*                     

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CATCAGAAATATGAAACTATGCAGTGCTTCGCTGTCTCTCAACCAAAGTCCATCAAAGAA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GAAGGAGAAGATTTGCAGTCCTGCTTGATTTGCGTGGCAAGAAGAGTTCCCATGAAGGAA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGACCAGTTCTTCCCTCATCAGAAAGTTTTACTACTCGCCAGGATCTCCAAGGCAAGATC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ACGTCTCTGGATACCAGCACCATGAGAGCAGCCATGAAACCAGGCTGGGAGGACCTGGTA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGAAGGTGTATTCAGAAGTTCCATGCGCAGCATGAAGGAGAATCTGTGTCCTATGCTAAG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGGCATCATCATGAAGTACTGAGACAAGGATTGGCATTCAGTCAAATCTATCGTTTTTCC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       TTGTCTGATGGCACTCTTGTTGCTGCACAAACGAAGAGCAAACTCATCCGTTCTCAGACT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ACTAATGAACCTCAACTTGTAATATCTTTACATATGCTTCACAGAGAGCAGAATGTGTGT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GTGATGAATCCGGATCTGACTGGACAAACGATGGGGAAGCCACTGAATCCAATTAGCTCT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AACAGCCCTGCCCATCAGGCCCTGTGCAGTGGGAACCCAGGTCAGGACATGACCCTCAGT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGCAATATAAATTTTCCCATAAATGGCCCAAAGGAACAAATGGGCATGCCCATGGGCAGG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       TTTGGTGGTTCTGGGGGAATGAACCATGTGTCAGGCATGCAAGCAACCACTCCTCAGGGT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGTAACTATGCACTCAAAATGAACAGCCCCTCACAAAGCAGCCCTGGCATGAATCCAGGA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CAGCCCACCTCCATGCTTTCACCAAGGCATCGCATGAGCCCTGGAGTGGCTGGCAGCCCT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CGAATCCCACCCAGTCAGTTTTCCCCTGCAGGAAGCTTGCATTCCCCTGTGGGAGTTTGC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGCAGCACAGGAAATAGCCATAGTTATACCAACAGCTCCCTCAATGCACTTCAGGCCCTC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGCGAGGGGCACGGGGTCTCATTAGGGTCATCGTTGGCTTCACCAGACCTAAAAATGGGC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AATTTGCAAAACTCCCCAGTTAATATGAATCCTCCCCCACTCAGCAAGATGGGAAGCTTG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GACTCAAAAGACTGTTTTGGACTATATGGGGAGCCCTCTGAAGGTACAACTGGACAAGCA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GAGAGCAGCTGCCATCCTGGAGAGCAAAAGGAAACAAATGACCCCAACCTGCCCCCGGCC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GTGAGCAGTGAGAGAGCTGACGGGCAGAGCAGACTGCATGACAGCAAAGGGCAGACCAAA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CTCCTGCAGCTGCTGACCACCAAATCTGATCAGATGGAGCCCTCGCCCTTAGCCAGCTCT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       TTGTCGGATACAAACAAAGACTCCACAGGTAGCTTGCCTGGTTCTGGGTCTACACATGGA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ACCTCGCTCAAGGAGAAGCATAAAATTTTGCACAGACTCTTGCAGGACAGCAGTTCCCCT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GTGGACTTGGCCAAGTTAACAGCAGAAGCCACAGGCAAAGACCTGAGCCAGGAGTCCAGC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGCACAGCTCCTGGATCAGAAGTGACTATTAAACAAGAGCCGGTGAGCCCCAAGAAGAAA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GAGAATGCACTACTTCGCTATTTGCTAGATAAAGATGATACTAAAGATATTGGTTTACCA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GAAATAACCCCCAAACTTGAGAGACTGGACAGTAAGACAGATCCTGCCAGTAACACAAAA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       TTAATAGCAATGAAAACTGAGAAGGAGGAGATGAGCTTTGAGCCTGGTGACCAGCCTGGC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGTGAGCTGGACAACTTGGAGGAGATTTTGGATGATTTGCAGAATAGTCAATTACCACAG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CTTTTCCCAGACACGAGGCCAGGCGCCCCTGCTGGATCAGTTGACAAGCAAGCCATCATC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AATGACCTCATGCAACTCACAGCTGAAAACAGCCCTGTCACACCTGTTGGAGCCCAGAAA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ACAGCACTGCGAATTTCACAGAGCACTTTTAATAACCCACGACCAGGGCAACTGGGCAGG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       TTATTGCCAAACCAGAATTTACCACTTGACATCACATTGCAAAGCCCAACTGGTGCTGGA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CCTTTCCCACCAATCAGAAACAGTAGTCCCTACTCAGTGATACCTCAGCCAGGAATGATG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GGTAATCAAGGGATGATAGGAAACCAAGGAAATTTAGGGAACAGTAGCACAGGAATGATT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GGTAACAGTGCTTCTCGGCCTACTATGCCATCTGGAGAATGGGCACCGCAGAGTTCGGCT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GTGAGAGTCACCTGTGCTGCTACCACCAGTGCCATGAACCGGCCAGTCCAAGGAGGTATG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ATTCGGAACCCAGCAGCCAGCATCCCCATGAGGCCCAGCAGCCAGCCTGGCCAAAGACAG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ACGCTTCAGTCTCAGGTCATGAATATAGGGCCATCTGAATTAGAGATGAACATGGGGGGA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CCTCAGTATAGCCAACAACAAGCTCCTCCAAATCAGACTGCCCCATGGCCTGAAAGCATC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CTGCCTATAGACCAGGCGTCTTTTGCCAGCCAAAACAGGCAGCCATTTGGCAGTTCTCCA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GATGACTTGCTATGTCCACATCCTGCAGCTGAGTCTCCGAGTGATGAGGGAGCTCTCCTG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GACCAGCTGTATCTGGCCTTGCGGAATTTTGATGGCCTGGAGGAGATTGATAGAGCCTTA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GGAATACCCGAACTGGTCAGCCAGAGCCAAGCAGTAGATCCAGAACAGTTCTCAAGTCAG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GATTCCAACATCATGCTGGAGCAGAAGGCGCCCGTTTTCCCACAGCAGTATGCATCTCAG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GCACAAATGGCCCAGGGTAGCTATTCTCCCATGCAAGATCCAAACTTTCACACCATGGGA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CAGCGGCCTAGTTATGCCACACTCCGTATGCAGCCCAGACCGGGCCTCAGGCCCACGGGC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CTAGTGCAGAACCAGCCAAATCAACTAAGACTTCAACTTCAGCATCGCCTCCAAGCACAG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CAGAATCGCCAGCCACTTATGAATCAAATCAGCAATGTTTCCAATGTGAACTTGACTCTG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AGGCCTGGAGTACCAACACAGGCACCTATTAATGCACAGATGCTGGCCCAGAGACAGAGG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GAAATCCTGAACCAGCATCTTCGACAGAGACAAATGCATCAGCAACAGCAAGTTCAGCAA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CGAACTTTGATGATGAGAGGACAAGGGTTGAATATGACACCAAGCATGGTGGCTCCTAGT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       GGTATGCCAGCAACTATGAGCAACCCTCGGATTCCCCAGGCAAATGCACAGCAGTTTCCA
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       TTTCCTCCAAACTACGGAATAAGTCAGCAACCTGATCCAGGCTTTACTGGGGCTACGACT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       CCCCAGAGCCCACTTATGTCACCCCGAATGGCACATACACAGAGTCCCATGATGCAACAG
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       TCTCAGGCCAACCCAGCCTATCAGGCCCCCTCCGACATAAATGGATGGGCGCAGGGGAAC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ATGGGCGGAAACAGCATGTTTTCCCAGCAGTCCCCACCACACTTTGGGCAGCAAGCAAAC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ACCAGCATGTACAGTAACAACATGAACATCAATGTGTCCATGGCGACCAACACAGGTGGC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ATGAGCAGCATGAACCAGATGACAGGACAGATCAGCATGACCTCAGTGACCTCCGTGCCT
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       ACGTCAGGGCTGTCCTCCATGGGTCCCGAGCAGGTTAATGATCCTGCTCTGAGGGGAGGC
                                                                              

HsCD00539728      ------------------------------------------------------------
NM_006540.2       AACCTGTTCCCAAACCAGCTGCCTGGAATGGATATGATTAAGCAGGAGGGAGACACAACA
                                                                              

HsCD00539728      ---------------
NM_006540.2       CGGAAATATTGCTGA
                                 

Pathways

NCI : Coregulation of Androgen receptor activity
NCI : Glucocorticoid receptor regulatory network
NCI : HIF-1-alpha transcription factor network
NCI : Regulation of Androgen receptor activity
NCI : Regulation of nuclear SMAD2/3 signaling
NCI : Regulation of nuclear beta catenin signaling and target gene transcription
NCI : Retinoic acid receptors-mediated signaling
NCI : Validated nuclear estrogen receptor alpha network
NCI : Validated nuclear estrogen receptor beta network
Reactome : Activation of gene expression by SREBF (SREBP)
Reactome : BMAL1:CLOCK,NPAS2 activates circadian gene expression
Reactome : Circadian Clock
Reactome : Developmental Biology
Reactome : Fatty acid, triacylglycerol, and ketone body metabolism
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Mitochondrial biogenesis
Reactome : Organelle biogenesis and maintenance
Reactome : PPARA activates gene expression
Reactome : REV-ERBA represses gene expression
Reactome : RORA activates circadian gene expression
Reactome : Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome : Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
Reactome : Transcriptional activation of mitochondrial biogenesis
Reactome : Transcriptional regulation of white adipocyte differentiation
Reactome : YAP1- and WWTR1 (TAZ)-stimulated gene expression
WikiPathway : Adipogenesis
WikiPathway : Androgen receptor signaling pathway

Protein

SMART domain : PAS : PAS domain
SMART domain : HLH : helix loop helix domain
Structural Annotation Wiki : HR3538F
UniProt : Q15596
HPRD : 09066

Clone

Cloning Information : 620031
HIP Master Clone ID : 517012
Original Clone ID : HR3538F-27-182-14.7

Experimental

TargetTrack - Experimental data : HR3538F

Organism


TAX_ID : 9606
Species Specific ID: 10499

Genome

Chromosome : 8
Map Location : 8q13.3
Ensembl : ENSG00000140396

Reagents

Labome : NCOA2-antibody