mltD (S. flexneri )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  mltD
Gene Name:  membrane-bound lytic murein transglycosylase D
Original Clone ID:
SfR231A-21.3
Keyword:
None
Species:
Shigella flexneri
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Rutgers University
PSI
Northeast Structural Genomics Consortium
SfCD00540011
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1299nts
Open reading frame: 51 to 1349

TGCGAATTCCCTCTGAATAATTTTGTTTAACTTTAGAAGGAGATATACATATGCAGAGTA
CCGGCAACGTTCAACAGCACGCACAGAGCCTTTCTGCAGCTGGTCAAGGGGAAGCAGCAA
AGTTTACAAGTCAGGCACGATGGATGGACGATGGGACGTCTATCGCGCCAGATGGTGACT
TGTGGGCTTTCATTGGCGACGAGCTAAAGATGGGAATTCCGGAAAATGACCGGATTCGCG
AACAGAAACAGAAATATTTACGCAATAAGAGCTATCTCCACGATGTAACTTTACGGGCAG
AGCCGTATATGTACTGGATAGCAGGGCAAGTTAAAAAACGTAACATGCCTATGGAACTGG
TACTACTACCCATAGTGGAGAGCGCTTTTGATCCTCACGCAACGTCTGGCGCCAATGCCG
CAGGCATCTGGCAGATCATTCCGAGCACGGGGCGCAATTATGGTTTGAAACAGACCCGCA
ATTATGACGCGCGTCGCGATGTTGTTGCTTCAACAACTGCCGCGCTGAATATGATGCAGC
GTCTGAACAAAATGTTTGACGACGACTGGCTTCTGACCGTAGCGGCTTATAACAGCGGCG
AAGGTCGGGTCATGAAGGCAATTAAAACGAACAAAGCGCGTGGGAAATCCACGGACTTCT
GGTCGTTACCGTTGCCGCAGGAAACGAAGCAGTACGTGCCTAAAATGCTGGCATTGAGTG
ATATTCTCAAAAACAGCAAGCGTTATGGCGTACGTCTGCCAACGACCGATGAAAGCCGTG
CTCTGGCGCGTGTGCACCTGAGTAGCCCGGTTGAAATGGCGAAGGTTGCAGATATGGCGG
GGATTTCCGTCAGCAAGCTGAAGACATTCAACGCTGGCGTGAAAGGCTCCACGCTGGGCG
CAAGTGGGCCGCAGTACGTGATGGTGCCAAAGAAGCATGCAGATCAACTGCGTGAATCTC
TCGCTTCAGGCGAAATTGCTGCTGTACAGTCGACGCTGGTTGCCAACAATACGCCGCTTA
ACAGCCGTGTTTACACCGTACGCTCTGGCGACACGCTTTCAAGCATCGCTTCACGTCTCG
GCGTAAGCACCAAAGATTTGCAGCAGTGGAACAAACTGCGCGGATCTAAGCTGAAGCCAG
GCCAAAGTTTGACGATTGGTGCACAGCGACTGGCAAACAACAGCGATAGCATTACGTATC
GTGTGCGCAAAGGCGATTCGCTTTCAAGCATTGCTAAACGCCACGGCGTGAACATCAAAG
ATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGACAAGCTGACGTTGT
TTGTGAAAAACAACAACATGCCAGATTCC

Protein sequence predicted based on nucleotide sequence

MQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLWAFIGDELKMGIPEND
RIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSG
ANAAGIWQIIPSTGRNYGLKQTRNYDARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAY
NSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTD
ESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQL
RESLASGEIAAVQSTLVANNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSK
LKPGQSLTIGAQRLANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDK
LTLFVKNNNMPDS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in SfR231A
Recommended expression in:
Not Applicable