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HDGF (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HDGF
Gene Name:  hepatoma-derived growth factor
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH021503.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000543

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 723nts         Open reading frame : 1 to 723
Reference Sequence Alignment

ATGTCGCGATCCAACCGGCAGAAGGAGTACAAATGCGGGGACCTGGTGTTCGCCAAGATG
AAGGGCTACCCACACTGGCCGGCCCGGATTGACGAGATGCCTGAGGCTGCCGTGAAATCA
ACAGCCAACAAATACCAAGTCTTTTTTTTCGGGACCCACGAGACGGCATTCCTGGGCCCC
AAAGACCTCTTCCCTTACGAGGAATCCAAGGAGAAGTTTGGCAAGCCCAACAAGAGGAAA
GGGTTCAGCGAGGGGCTGTGGGAGATCGAGAACAACCCTACTGTCAAGGCTTCCGGCTAT
CAGTCCTCCCAGAAAAAGAGCTGTGTGGAAGAGCCTGAACCAGAGCCCGAAGCTGCAGAG
GGTGACGGTGATAAGAAGGGGAATGCAGAGGGCAGCAGCGACGAGGAAGGGAAGCTGGTC
ATTGATGAGCCAGCCAAGGAGAAGAACGAGAAAGGAGCGTTGAAGAGGAGAGCAGGGGAC
TTGCTGGAGGACTCTCCTAAACGTCCCAAGGAGGCAGAAAACCCTGAAGGAGAGGAGAAG
GAGGCAGCCACCTTGGAGGTTGAGAGGCCCCTTCCTATGGAGGTGGAAAAGAATAGCACC
CTCTCTGAGCCCGGCTCTGGCCGGGGGCCTCCCCAAGAGGAAGAAGAAGAGGAGGATGAA
GAGGAAGAGGCTACCAAGGAAGATGCTGAGGCCCCAGGCATCAGAGATCATGAGAGCCTG
TTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1038
Start on reference sequence 316
Mutations:
None
Discrepancy:
c602t,P201L
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HDGF
Symbol Nomenclature : HDGF
SYNONYM : HMG1L2
Designation : HMG-1L2|high mobility group protein 1-like 2
Full Nomenclature : hepatoma-derived growth factor
GENEID : 3068
Locus Tag : RP11-66D17.10-003
GI : 61366085
GenBank Accession : AY890873
HGNC : 4856
MIM : 600339
Vega : OTTHUMG00000041295
Target GenBank: NM_004494

 

Reference Sequence Alignment

  

HsCD00000543        ------------------------------------------------ATGTCGCGATCC
NM_001126050.1      ATGCACCCGGAAGGTGGCCAATTTGTGCCTCAACTCCTTGGCCATCTCCTGGCTACCAAA
NM_001126051.1      ------------------------------------------------------------
NM_004494.2         ------------------------------------------------ATGTCGCGATCC
                                                                                

HsCD00000543        AACCGGCAGAAGGAGTACAAATGCGGGGACCTGGTGTTCGCCAAGATGAAGGGCTACCCA
NM_001126050.1      CTCAAGCGTTTCCTCCTTAGCAAAGGCGGACGTAGGGCTCAAATCCCCGACGTTTCCAGG
NM_001126051.1      ATGGAGCAGAGGGCAGGCGGAAACCGTGTACAGACGTCCACACTTAACTGCG--------
NM_004494.2         AACCGGCAGAAGGAGTACAAATGCGGGGACCTGGTGTTCGCCAAGATGAAGGGCTACCCA
                    .:  .**. :        ...:.. * * .*  . *    ...: .   . *        

HsCD00000543        CACTGGCCGGCCCGGATTGACGAGATGCCTGAGGCTGCCGTGAAATCAACAGCCAACAAA
NM_001126050.1      GCCACCCCCCATACGATTGACGAGATGCCTGAGGCTGCCGTGAAATCAACAGCCAACAAA
NM_001126051.1      -CTGGGGCCGCGGTGATTGACGAGATGCCTGAGGCTGCCGTGAAATCAACAGCCAACAAA
NM_004494.2         CACTGGCCGGCCCGGATTGACGAGATGCCTGAGGCTGCCGTGAAATCAACAGCCAACAAA
                     .     *  .   **********************************************

HsCD00000543        TACCAAGTCTTTTTTTTCGGGACCCACGAGACGGCATTCCTGGGCCCCAAAGACCTCTTC
NM_001126050.1      TACCAAGTCTTTTTTTTCGGGACCCACGAGACGGCATTCCTGGGCCCCAAAGACCTCTTC
NM_001126051.1      TACCAAGTCTTTTTTTTCGGGACCCACGAGACGGCATTCCTGGGCCCCAAAGACCTCTTC
NM_004494.2         TACCAAGTCTTTTTTTTCGGGACCCACGAGACGGCATTCCTGGGCCCCAAAGACCTCTTC
                    ************************************************************

HsCD00000543        CCTTACGAGGAATCCAAGGAGAAGTTTGGCAAGCCCAACAAGAGGAAAGGGTTCAGCGAG
NM_001126050.1      CCTTACGAGGAATCCAAGGAGAAGTTTGGCAAGCCCAACAAGAGGAAAGGGTTCAGCGAG
NM_001126051.1      CCTTACGAGGAATCCAAGGAGAAGTTTGGCAAGCCCAACAAGAGGAAAGGGTTCAGCGAG
NM_004494.2         CCTTACGAGGAATCCAAGGAGAAGTTTGGCAAGCCCAACAAGAGGAAAGGGTTCAGCGAG
                    ************************************************************

HsCD00000543        GGGCTGTGGGAGATCGAGAACAACCCTACTGTCAAGGCTTCCGGCTATCAGTCCTCCCAG
NM_001126050.1      GGGCTGTGGGAGATCGAGAACAACCCTACTGTCAAGGCTTCCGGCTATCAGTCCTCCCAG
NM_001126051.1      GGGCTGTGGGAGATCGAGAACAACCCTACTGTCAAGGCTTCCGGCTATCAGTCCTCCCAG
NM_004494.2         GGGCTGTGGGAGATCGAGAACAACCCTACTGTCAAGGCTTCCGGCTATCAGTCCTCCCAG
                    ************************************************************

HsCD00000543        AAAAAGAGCTGTGTGGAAGAGCCTGAACCAGAGCCCGAAGCTGCAGAGGGTGACGGTGAT
NM_001126050.1      AAAAAGAGCTGTGTGGAAGAGCCTGAACCAGAGCCCGAAGCTGCAGAGGGTGACGGTGAT
NM_001126051.1      AAAAAGAGCTGTGTGGAAGAGCCTGAACCAGAGCCCGAAGCTGCAGAGGGTGACGGTGAT
NM_004494.2         AAAAAGAGCTGTGTGGAAGAGCCTGAACCAGAGCCCGAAGCTGCAGAGGGTGACGGTGAT
                    ************************************************************

HsCD00000543        AAGAAGGGGAATGCAGAGGGCAGCAGCGACGAGGAAGGGAAGCTGGTCATTGATGAGCCA
NM_001126050.1      AAGAAGGGGAATGCAGAGGGCAGCAGCGACGAGGAAGGGAAGCTGGTCATTGATGAGCCA
NM_001126051.1      AAGAAGGGGAATGCAGAGGGCAGCAGCGACGAGGAAGGGAAGCTGGTCATTGATGAGCCA
NM_004494.2         AAGAAGGGGAATGCAGAGGGCAGCAGCGACGAGGAAGGGAAGCTGGTCATTGATGAGCCA
                    ************************************************************

HsCD00000543        GCCAAGGAGAAGAACGAGAAAGGAGCGTTGAAGAGGAGAGCAGGGGACTTGCTGGAGGAC
NM_001126050.1      GCCAAGGAGAAGAACGAGAAAGGAGCGTTGAAGAGGAGAGCAGGGGACTTGCTGGAGGAC
NM_001126051.1      GCCAAGGAGAAGAACGAGAAAGGAGCGTTGAAGAGGAGAGCAGGGGACTTGCTGGAGGAC
NM_004494.2         GCCAAGGAGAAGAACGAGAAAGGAGCGTTGAAGAGGAGAGCAGGGGACTTGCTGGAGGAC
                    ************************************************************

HsCD00000543        TCTCCTAAACGTCCCAAGGAGGCAGAAAACCCTGAAGGAGAGGAGAAGGAGGCAGCCACC
NM_001126050.1      TCTCCTAAACGTCCCAAGGAGGCAGAAAACCCTGAAGGAGAGGAGAAGGAGGCAGCCACC
NM_001126051.1      TCTCCTAAACGTCCCAAGGAGGCAGAAAACCCTGAAGGAGAGGAGAAGGAGGCAGCCACC
NM_004494.2         TCTCCTAAACGTCCCAAGGAGGCAGAAAACCCTGAAGGAGAGGAGAAGGAGGCAGCCACC
                    ************************************************************

HsCD00000543        TTGGAGGTTGAGAGGCCCCTTCCTATGGAGGTGGAAAAGAATAGCACCCTCTCTGAGCCC
NM_001126050.1      TTGGAGGTTGAGAGGCCCCTTCCTATGGAGGTGGAAAAGAATAGCACCCCCTCTGAGCCC
NM_001126051.1      TTGGAGGTTGAGAGGCCCCTTCCTATGGAGGTGGAAAAGAATAGCACCCCCTCTGAGCCC
NM_004494.2         TTGGAGGTTGAGAGGCCCCTTCCTATGGAGGTGGAAAAGAATAGCACCCCCTCTGAGCCC
                    ************************************************* **********

HsCD00000543        GGCTCTGGCCGGGGGCCTCCCCAAGAGGAAGAAGAAGAGGAGGATGAAGAGGAAGAGGCT
NM_001126050.1      GGCTCTGGCCGGGGGCCTCCCCAAGAGGAAGAAGAAGAGGAGGATGAAGAGGAAGAGGCT
NM_001126051.1      GGCTCTGGCCGGGGGCCTCCCCAAGAGGAAGAAGAAGAGGAGGATGAAGAGGAAGAGGCT
NM_004494.2         GGCTCTGGCCGGGGGCCTCCCCAAGAGGAAGAAGAAGAGGAGGATGAAGAGGAAGAGGCT
                    ************************************************************

HsCD00000543        ACCAAGGAAGATGCTGAGGCCCCAGGCATCAGAGATCATGAGAGCCTGTTG
NM_001126050.1      ACCAAGGAAGATGCTGAGGCCCCAGGCATCAGAGATCATGAGAGCCTGTAG
NM_001126051.1      ACCAAGGAAGATGCTGAGGCCCCAGGCATCAGAGATCATGAGAGCCTGTAG
NM_004494.2         ACCAAGGAAGATGCTGAGGCCCCAGGCATCAGAGATCATGAGAGCCTGTAG
                    *************************************************:*

Pathways

Reactome : IRE1alpha activates chaperones
Reactome : Metabolism of proteins
Reactome : Unfolded Protein Response (UPR)
Reactome : XBP1(S) activates chaperone genes

Protein

SMART domain : PWWP : domain with conserved PWWP motif
UniProt : P51858
UniProt : Q5SZ07
UniProt : Q5SZ08
HPRD : 02079

Clone

Cloning Information : 21503
HIP Master Clone ID : 40063
Original Clone ID : FLH021503.01L

Organism


TAX_ID : 9606
Species Specific ID: 3068

Genome

Chromosome : 1
Map Location : 1q23.1
Ensembl : ENSG00000143321

Reagents

Labome : HDGF-antibody