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NT5C2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  NT5C2
Gene Name:  5'-nucleotidase, cytosolic II
Sequence             
Original Clone ID: 5434.1.10_GO.34926
Keyword: full-length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00546367

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1704nts         Open reading frame : 51 to 1754
Reference Sequence Alignment

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCTCAACCTCCTGGAGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTA
ACATGGATAAGCATGCCCTGAAAAAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGA
ACCGAAGTTTAGCAATGGAAAAGATAAAGTGTTTTGGTTTTGATATGGATTATACCCTTG
CTGTGTACAAGTCCCCAGAGTATGAGTCCCTTGGTTTTGAGCTTACTGTGGAGAGATTAG
TTTCTATTGGCTATCCCCAGGAGTTGCTCAGCTTTGCTTATGATTCTACATTCCCTACCA
GGGGACTTGTCTTTGACACACTGTATGGAAATCTTTTGAAAGTCGATGCCTATGGAAACC
TCTTGGTCTGTGCACATGGATTTAACTTTATAAGGGGACCAGAAACTAGAGAACAGTATC
CAAATAAATTTATCCAGCGAGATGATACTGAAAGATTTTACATTCTGAACACACTATTCA
ACCTACCAGAGACCTACCTGTTGGCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGAT
ATACCAGTTGTGAAACAGGATTTAAAGATGGGGACCTCTTCATGTCCTACCGGAGTATGT
TCCAGGATGTAAGAGATGCTGTTGACTGGGTTCATTACAAGGGCTCCCTTAAGGAAAAGA
CAGTTGAAAATCTTGAGAAGTATGTAGTCAAAGATGGAAAACTGCCTTTGCTTCTGAGCC
GGATGAAGGAAGTAGGGAAAGTATTTCTTGCTACCAACAGTGACTATAAATATACAGATA
AAATTATGACTTACCTGTTTGACTTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGAC
CATGGCAGTCCTACTTTGACTTGATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAG
AAGGCACAGTACTGCGTCAGGTGGATACTAAAACTGGCAAGCTGAAAATTGGTACCTACA
CAGGGCCCCTACAGCATGGTATCGTCTACTCAGGAGGTTCTTCTGATACGATCTGTGACC
TGTTGGGAGCCAAGGGAAAAGACATTTTGTATATTGGAGATCACATTTTTGGGGACATTT
TAAAATCAAAGAAACGGCAAGGGTGGCGAACTTTTTTGGTGATTCCTGAACTCGCACAGG
AGCTACATGTCTGGACTGACAAGAGTTCACTTTTCGAAGAACTTCAGAGCTTGGATATTT
TCTTGGCTGAACTCTACAAGCATCTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTT
CCATCCAGAGACGTATTAAGAAAGTAACTCATGACATGGACATGTGCTATGGGATGATGG
GAAGCCTGTTTCGCAGTGGCTCCCGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATG
CTGACCTCTATGCAGCATCTTTCATCAACCTGCTGTATTACCCTTTCAGCTACCTCTTCA
GGGCTGCCCATGTCTTGATGCCTCATGAATCAACGGTGGAGCACACACACGTAGATATCA
ATGAGATGGAGTCTCCTCTTGCCACCCGGAACCGCACATCAGTGGATTTCAAAGACACTG
ACTACAAGCGGCACCAGCTGACACGGTCAATTAGTGAGATTAAACCTCCCAACCTCTTCC
CACTGGCCCCCCAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGG
AGGAGGAAGAATAATAGGACCCAGCTTTCTTGTA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
GACCCAGCTTTCTTGTACAAAGTTGGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.34926
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NT5C2
Symbol Nomenclature : NT5C2
SYNONYM : GMP; NT5B; PNT5; cN-II
Designation : 5'-nucleotidase (purine), cytosolic type B|IMP-specific 5'-NT|cytosolic 5'-nucleotidase II|cytosolic purine 5'-nucleotidase|purine 5' nucleotidase
Full Nomenclature : 5'-nucleotidase, cytosolic II
GENEID : 22978
Locus Tag : RP11-30H12.3
GI : 12804389
GenBank Accession : BC001595
HGNC : 8022
MIM : 600417
Vega : OTTHUMG00000018981
Target GenBank: BC001595

 

Reference Sequence Alignment

  

HsCD00546367        ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
NM_001134373.2      ------------------------------------------------------------
NM_012229.4         ------------------------------------------------------------
                                                                                

HsCD00546367        ACTTCCAGTCCTCAACCTCCTGGAGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTA
NM_001134373.2      --------ATGTCAACCTCCTGGAGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTA
NM_012229.4         --------ATGTCAACCTCCTGGAGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTA
                            :  *************************************************

HsCD00546367        ACATGGATAAGCATGCCCTGAAAAAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGA
NM_001134373.2      ACATGGATAAGCATGCCCTGAAAAAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGA
NM_012229.4         ACATGGATAAGCATGCCCTGAAAAAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGA
                    ************************************************************

HsCD00546367        ACCGAAGTTTAGCAATGGAAAAGATAAAGTGTTTTGGTTTTGATATGGATTATACCCTTG
NM_001134373.2      ACCGAAGTTTAGCAATGGAAAAGATAAAGTGTTTTGGTTTTGATATGGATTATACCCTTG
NM_012229.4         ACCGAAGTTTAGCAATGGAAAAGATAAAGTGTTTTGGTTTTGATATGGATTATACCCTTG
                    ************************************************************

HsCD00546367        CTGTGTACAAGTCCCCAGAGTATGAGTCCCTTGGTTTTGAGCTTACTGTGGAGAGATTAG
NM_001134373.2      CTGTGTACAAGTCCCCAGAGTATGAGTCCCTTGGTTTTGAGCTTACTGTGGAGAGATTAG
NM_012229.4         CTGTGTACAAGTCCCCAGAGTATGAGTCCCTTGGTTTTGAGCTTACTGTGGAGAGATTAG
                    ************************************************************

HsCD00546367        TTTCTATTGGCTATCCCCAGGAGTTGCTCAGCTTTGCTTATGATTCTACATTCCCTACCA
NM_001134373.2      TTTCTATTGGCTATCCCCAGGAGTTGCTCAGCTTTGCTTATGATTCTACATTCCCTACCA
NM_012229.4         TTTCTATTGGCTATCCCCAGGAGTTGCTCAGCTTTGCTTATGATTCTACATTCCCTACCA
                    ************************************************************

HsCD00546367        GGGGACTTGTCTTTGACACACTGTATGGAAATCTTTTGAAAGTCGATGCCTATGGAAACC
NM_001134373.2      GGGGACTTGTCTTTGACACACTGTATGGAAATCTTTTGAAAGTCGATGCCTATGGAAACC
NM_012229.4         GGGGACTTGTCTTTGACACACTGTATGGAAATCTTTTGAAAGTCGATGCCTATGGAAACC
                    ************************************************************

HsCD00546367        TCTTGGTCTGTGCACATGGATTTAACTTTATAAGGGGACCAGAAACTAGAGAACAGTATC
NM_001134373.2      TCTTGGTCTGTGCACATGGATTTAACTTTATAAGGGGACCAGAAACTAGAGAACAGTATC
NM_012229.4         TCTTGGTCTGTGCACATGGATTTAACTTTATAAGGGGACCAGAAACTAGAGAACAGTATC
                    ************************************************************

HsCD00546367        CAAATAAATTTATCCAGCGAGATGATACTGAAAGATTTTACATTCTGAACACACTATTCA
NM_001134373.2      CAAATAAATTTATCCAGCGAGATGATACTGAAAGATTTTACATTCTGAACACACTATTCA
NM_012229.4         CAAATAAATTTATCCAGCGAGATGATACTGAAAGATTTTACATTCTGAACACACTATTCA
                    ************************************************************

HsCD00546367        ACCTACCAGAGACCTACCTGTTGGCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGAT
NM_001134373.2      ACCTACCAGAGACCTACCTGTTGGCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGAT
NM_012229.4         ACCTACCAGAGACCTACCTGTTGGCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGAT
                    ************************************************************

HsCD00546367        ATACCAGTTGTGAAACAGGATTTAAAGATGGGGACCTCTTCATGTCCTACCGGAGTATGT
NM_001134373.2      ATACCAGTTGTGAAACAGGATTTAAAGATGGGGACCTCTTCATGTCCTACCGGAGTATGT
NM_012229.4         ATACCAGTTGTGAAACAGGATTTAAAGATGGGGACCTCTTCATGTCCTACCGGAGTATGT
                    ************************************************************

HsCD00546367        TCCAGGATGTAAGAGATGCTGTTGACTGGGTTCATTACAAGGGCTCCCTTAAGGAAAAGA
NM_001134373.2      TCCAGGATGTAAGAGATGCTGTTGACTGGGTTCATTACAAGGGCTCCCTTAAGGAAAAGA
NM_012229.4         TCCAGGATGTAAGAGATGCTGTTGACTGGGTTCATTACAAGGGCTCCCTTAAGGAAAAGA
                    ************************************************************

HsCD00546367        CAGTTGAAAATCTTGAGAAGTATGTAGTCAAAGATGGAAAACTGCCTTTGCTTCTGAGCC
NM_001134373.2      CAGTTGAAAATCTTGAGAAGTATGTAGTCAAAGATGGAAAACTGCCTTTGCTTCTGAGCC
NM_012229.4         CAGTTGAAAATCTTGAGAAGTATGTAGTCAAAGATGGAAAACTGCCTTTGCTTCTGAGCC
                    ************************************************************

HsCD00546367        GGATGAAGGAAGTAGGGAAAGTATTTCTTGCTACCAACAGTGACTATAAATATACAGATA
NM_001134373.2      GGATGAAGGAAGTAGGGAAAGTATTTCTTGCTACCAACAGTGACTATAAATATACAGATA
NM_012229.4         GGATGAAGGAAGTAGGGAAAGTATTTCTTGCTACCAACAGTGACTATAAATATACAGATA
                    ************************************************************

HsCD00546367        AAATTATGACTTACCTGTTTGACTTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGAC
NM_001134373.2      AAATTATGACTTACCTGTTTGACTTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGAC
NM_012229.4         AAATTATGACTTACCTGTTTGACTTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGAC
                    ************************************************************

HsCD00546367        CATGGCAGTCCTACTTTGACTTGATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAG
NM_001134373.2      CATGGCAGTCCTACTTTGACTTGATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAG
NM_012229.4         CATGGCAGTCCTACTTTGACTTGATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAG
                    ************************************************************

HsCD00546367        AAGGCACAGTACTGCGTCAGGTGGATACTAAAACTGGCAAGCTGAAAATTGGTACCTACA
NM_001134373.2      AAGGCACAGTACTGCGTCAGGTGGATACTAAAACTGGCAAGCTGAAAATTGGTACCTACA
NM_012229.4         AAGGCACAGTACTGCGTCAGGTGGATACTAAAACTGGCAAGCTGAAAATTGGTACCTACA
                    ************************************************************

HsCD00546367        CAGGGCCCCTACAGCATGGTATCGTCTACTCAGGAGGTTCTTCTGATACGATCTGTGACC
NM_001134373.2      CAGGGCCCCTACAGCATGGTATCGTCTACTCAGGAGGTTCTTCTGATACGATCTGTGACC
NM_012229.4         CAGGGCCCCTACAGCATGGTATCGTCTACTCAGGAGGTTCTTCTGATACGATCTGTGACC
                    ************************************************************

HsCD00546367        TGTTGGGAGCCAAGGGAAAAGACATTTTGTATATTGGAGATCACATTTTTGGGGACATTT
NM_001134373.2      TGTTGGGAGCCAAGGGAAAAGACATTTTGTATATTGGAGATCACATTTTTGGGGACATTT
NM_012229.4         TGTTGGGAGCCAAGGGAAAAGACATTTTGTATATTGGAGATCACATTTTTGGGGACATTT
                    ************************************************************

HsCD00546367        TAAAATCAAAGAAACGGCAAGGGTGGCGAACTTTTTTGGTGATTCCTGAACTCGCACAGG
NM_001134373.2      TAAAATCAAAGAAACGGCAAGGGTGGCGAACTTTTTTGGTGATTCCTGAACTCGCACAGG
NM_012229.4         TAAAATCAAAGAAACGGCAAGGGTGGCGAACTTTTTTGGTGATTCCTGAACTCGCACAGG
                    ************************************************************

HsCD00546367        AGCTACATGTCTGGACTGACAAGAGTTCACTTTTCGAAGAACTTCAGAGCTTGGATATTT
NM_001134373.2      AGCTACATGTCTGGACTGACAAGAGTTCACTTTTCGAAGAACTTCAGAGCTTGGATATTT
NM_012229.4         AGCTACATGTCTGGACTGACAAGAGTTCACTTTTCGAAGAACTTCAGAGCTTGGATATTT
                    ************************************************************

HsCD00546367        TCTTGGCTGAACTCTACAAGCATCTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTT
NM_001134373.2      TCTTGGCTGAACTCTACAAGCATCTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTT
NM_012229.4         TCTTGGCTGAACTCTACAAGCATCTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTT
                    ************************************************************

HsCD00546367        CCATCCAGAGACGTATTAAGAAAGTAACTCATGACATGGACATGTGCTATGGGATGATGG
NM_001134373.2      CCATCCAGAGACGTATTAAGAAAGTAACTCATGACATGGACATGTGCTATGGGATGATGG
NM_012229.4         CCATCCAGAGACGTATTAAGAAAGTAACTCATGACATGGACATGTGCTATGGGATGATGG
                    ************************************************************

HsCD00546367        GAAGCCTGTTTCGCAGTGGCTCCCGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATG
NM_001134373.2      GAAGCCTGTTTCGCAGTGGCTCCCGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATG
NM_012229.4         GAAGCCTGTTTCGCAGTGGCTCCCGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATG
                    ************************************************************

HsCD00546367        CTGACCTCTATGCAGCATCTTTCATCAACCTGCTGTATTACCCTTTCAGCTACCTCTTCA
NM_001134373.2      CTGACCTCTATGCAGCATCTTTCATCAACCTGCTGTATTACCCTTTCAGCTACCTCTTCA
NM_012229.4         CTGACCTCTATGCAGCATCTTTCATCAACCTGCTGTATTACCCTTTCAGCTACCTCTTCA
                    ************************************************************

HsCD00546367        GGGCTGCCCATGTCTTGATGCCTCATGAATCAACGGTGGAGCACACACACGTAGATATCA
NM_001134373.2      GGGCTGCCCATGTCTTGATGCCTCATGAATCAACGGTGGAGCACACACACGTAGATATCA
NM_012229.4         GGGCTGCCCATGTCTTGATGCCTCATGAATCAACGGTGGAGCACACACACGTAGATATCA
                    ************************************************************

HsCD00546367        ATGAGATGGAGTCTCCTCTTGCCACCCGGAACCGCACATCAGTGGATTTCAAAGACACTG
NM_001134373.2      ATGAGATGGAGTCTCCTCTTGCCACCCGGAACCGCACATCAGTGGATTTCAAAGACACTG
NM_012229.4         ATGAGATGGAGTCTCCTCTTGCCACCCGGAACCGCACATCAGTGGATTTCAAAGACACTG
                    ************************************************************

HsCD00546367        ACTACAAGCGGCACCAGCTGACACGGTCAATTAGTGAGATTAAACCTCCCAACCTCTTCC
NM_001134373.2      ACTACAAGCGGCACCAGCTGACACGGTCAATTAGTGAGATTAAACCTCCCAACCTCTTCC
NM_012229.4         ACTACAAGCGGCACCAGCTGACACGGTCAATTAGTGAGATTAAACCTCCCAACCTCTTCC
                    ************************************************************

HsCD00546367        CACTGGCCCCCCAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGG
NM_001134373.2      CACTGGCCCCCCAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGG
NM_012229.4         CACTGGCCCCCCAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGG
                    ************************************************************

HsCD00546367        AGGAGGAAGAATAATAGGACCCAGCTTTCTTGTA
NM_001134373.2      AGGAGGAAGAATAA--------------------
NM_012229.4         AGGAGGAAGAATAA--------------------
                    **************                    

Pathways

Reactome : Abacavir metabolism
Reactome : Abacavir transport and metabolism
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Purine catabolism
Reactome : Purine metabolism

Protein

Structural Annotation Wiki : GO.34926
HPRD : 02686

Clone

Cloning Information : 502918
HIP Master Clone ID : 414085
Original Clone ID : 5434.1.10_GO.34926

Experimental

TargetTrack - Experimental data : GO.34926

Organism


TAX_ID : 9606
Species Specific ID: 22978

Genome

Chromosome : 10
Map Location : 10q24.32
Ensembl : ENSG00000076685

Reagents

Labome : NT5C2-antibody