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lpg1283 (L. pneumophila subsp. pneumophila str. Philadelphia 1 ) in pSpeedET

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Explanation of Terms


Gene: Gene Symbol:  lpg1283
Gene Name:  lipoprotein NlpD
Sequence             
Original Clone ID: 75519
Keyword: partial cds
Species: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Type: cDNA
Vector Name: pSpeedET               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI:Biology
Joint Center for Structural Genomics
LpCD00546636

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 684nts        
Open reading frame : 51 to 734

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCTGTGGGACAA
GAAGTGATTTGGCTCCTGTTACCGAGCTAAAATGGCAGCCTTATTCAAAATATCAGAAAT
TCCATACGGTGCGATCAGGTGAGACCCTTTATGCTATAGCATTTCGTTATGATACTGATT
ATAGGCGGCTTGCGATCAACAATCACCTAAGGCCACCTTATTCTTTGCGAGTAGGACAAG
TTTTAAGTTTAAAAGGCATCGTGCCAAAATCAAATCACTTTCATTCAGCTCCCAGAGCTA
ACTTACATATTACCCATAAGCCAAAACAAAAAACTATTTATTCTCCAGCTTATAATGTAC
GTTCTTCCTCTGGTTGGCTATGGCCAGTTACTGGTCGTGTTGTTACAACATTCATTCCTT
CTCAGGGGAAAAAAGGCATAAATATTGCATGTAACAGAGGAGACAAAGTTAGAGCTTCAG
CGAGTGGAGTTGTGGCTTATGCTGGCAGCGGTTTGGCTGGGTATGGTAATTTAATTATCA
TTAAACACAGTAATGAATATTTAACTGCATACGGCAACAATGCCAGAAACCTGGTGACAG
AAGGACAGCGCGTTAATGCAGGGCAGGTAATAGCAGAAGCTGGGTTAATAGATAGATCAT
ACTGGGGTGTGCATTTTGAAATTAGAAGAGCAGGGGTGCCAGTTAATCCATTGAATTATC
TACAAAAAGGTTAACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTTCAGCCTGAT
ACAGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 418703
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSpeedET

pSpeedET in advanced viewed

Synonyms: pSpeed-ET
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
Comments: If this vector contains an insert, the ccdB death cassette will not be present.
Size (bp): 5464
Parent Vector: None
Empty Vector: EvNO00285791
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: PMID: 18004753
Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type origin of replication 3306 3988
inducible trxn activator protein gene araC araC coding sequence 4563 5438
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 1596 1805
promoter T7 T7 promoter 290 310
promoter araC araC promoter 256 285
protease cleavage site TEV TEV protease cleavage site 382 402
selectable marker CmR chloramphenicol resistance gene (CmR) (lost in with-insert form) 502 1158
selectable marker KanR kanamycin resistance gene 2396 3208
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2258 2284

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : lpg1283
GENEID : 3080334
Locus Tag : lpg1283
GI : 52841514
GenBank Accession : YP_095313.1
Target GenBank: None

 

Protein

SMART domain : LysM : Lysin motif
Structural Annotation Wiki : 418703

Clone

Cloning Information : 622134
Original Clone ID : 75519

Experimental

TargetTrack - Experimental data : 418703

Organism


TAX_ID : 272624
Species Specific ID: 3080334