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RBM17 (Homo sapiens) in pSpeedET (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RBM17
Gene Name:  RNA binding motif protein 17
Sequence             
Original Clone ID: 85785
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pSpeedET               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Joint Center for Structural Genomics
PSI:Biology
HsCD00547111

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1206nts         Open reading frame : 51 to 1256
Reference Sequence Alignment

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGTCCCTGT
ACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCCAAAAACTTCA
AACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCAAAGAGCCAAA
GGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGTGGCTCCTCAG
ATGACCGGCAAATTGTGGACACTCCACCGCATGTAGCAGCTGGGCTGAAGGATCCTGTTC
CCAGTGGGTTTTCTGCAGGGGAAGTTCTGATTCCCTTAGCTGACGAATATGACCCTATGT
TTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGAGGAACGACAGAGACAGCGGG
AGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGACAGACATGAAG
CAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGATTATGAGCGAG
AGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGA
AAGACAAAGAGTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCTCGATCACAGT
CTTCCAAAGCAGCCATTCCTCCCCCAGTGTACGAGGAACAAGACAGACCGAGATCTCCAA
CCGGACCTAGCAACTCCTTCCTCGCTAACATGGGGGGCACGGTGGCGCACAAGATCATGC
AGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCATGAGCAGGGCCTGAGCACTG
CCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGCGACGCCACAG
AGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTTAAGTGTCCTA
CTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGGGAGGTGGATGAAGACTTGGAAG
TTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTGATATTTGAAA
TTCCTGGTGCCCCTGATGATGAAGCAGTACGGATATTTTTAGAATTTGAGAGAGTTGAAT
CAGCAATTAAAGCGGTTGTTGACTTGAATGGGAGGTATTTTGGTGGACGGGTGGTAAAAG
CATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTTGGCAGAACAAGTTTAACGCG
ACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC

3' Linker Sequence:
TAA

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 421608
Recommended expression in: HK100
       DyNA Vector Map

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Vector Name: pSpeedET

pSpeedET in advanced viewed

Synonyms: pSpeed-ET
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
Comments: If this vector contains an insert, the ccdB death cassette will not be present.
Size (bp): 5464
Parent Vector: None
Empty Vector: EvNO00285791
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: PMID: 18004753
Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type origin of replication 3306 3988
inducible trxn activator protein gene araC araC coding sequence 4563 5438
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 1596 1805
promoter araC araC promoter 256 285
promoter T7 T7 promoter 290 310
protease cleavage site TEV TEV protease cleavage site 382 402
selectable marker CmR chloramphenicol resistance gene (CmR) (lost in with-insert form) 502 1158
selectable marker KanR kanamycin resistance gene 2396 3208
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn termination sequence rrnB rrnB T2 transcription terminator 2258 2284

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RBM17
Symbol Nomenclature : RBM17
SYNONYM : SPF45
Designation : 45 kDa-splicing factor|RNA-binding motif protein 17|splicing factor 45|splicing factor 45kDa
Full Nomenclature : RNA binding motif protein 17
GENEID : 84991
Locus Tag : RP11-414H17.9
GI : 25058249
GenBank Accession : BC039322
HGNC : 16944
MIM : 606935
Vega : OTTHUMG00000017618
Target GenBank: BC039322

 

Reference Sequence Alignment

  

HsCD00547111        CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGTCCCTGT
NM_001145547.1      --------------------------------------------------ATGTCCCTGT
NM_032905.4         --------------------------------------------------ATGTCCCTGT
                                                                      **********

HsCD00547111        ACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCCAAAAACTTCA
NM_001145547.1      ACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCCAAAAACTTCA
NM_032905.4         ACGATGACCTAGGAGTGGAGACCAGTGACTCAAAAACAGAAGGCTGGTCCAAAAACTTCA
                    ************************************************************

HsCD00547111        AACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCAAAGAGCCAAA
NM_001145547.1      AACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCAAAGAGCCAAA
NM_032905.4         AACTTCTGCAGTCTCAGCTTCAGGTGAAGAAGGCAGCTCTCACTCAGGCAAAGAGCCAAA
                    ************************************************************

HsCD00547111        GGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGTGGCTCCTCAG
NM_001145547.1      GGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGTGGCTCCTCAG
NM_032905.4         GGACGAAACAAAGTACAGTCCTCGCCCCAGTCATTGACCTGAAGCGAGGTGGCTCCTCAG
                    ************************************************************

HsCD00547111        ATGACCGGCAAATTGTGGACACTCCACCGCATGTAGCAGCTGGGCTGAAGGATCCTGTTC
NM_001145547.1      ATGACCGGCAAATTGTGGACACTCCACCGCATGTAGCAGCTGGGCTGAAGGATCCTGTTC
NM_032905.4         ATGACCGGCAAATTGTGGACACTCCACCGCATGTAGCAGCTGGGCTGAAGGATCCTGTTC
                    ************************************************************

HsCD00547111        CCAGTGGGTTTTCTGCAGGGGAAGTTCTGATTCCCTTAGCTGACGAATATGACCCTATGT
NM_001145547.1      CCAGTGGGTTTTCTGCAGGGGAAGTTCTGATTCCCTTAGCTGACGAATATGACCCTATGT
NM_032905.4         CCAGTGGGTTTTCTGCAGGGGAAGTTCTGATTCCCTTAGCTGACGAATATGACCCTATGT
                    ************************************************************

HsCD00547111        TTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGAGGAACGACAGAGACAGCGGG
NM_001145547.1      TTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGAGGAACGACAGAGACAGCGGG
NM_032905.4         TTCCTAATGATTATGAGAAAGTAGTGAAGCGCCAAAGAGAGGAACGACAGAGACAGCGGG
                    ************************************************************

HsCD00547111        AGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGACAGACATGAAG
NM_001145547.1      AGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGACAGACATGAAG
NM_032905.4         AGCTGGAAAGACAAAAGGAAATAGAAGAAAGGGAAAAAAGGCGTAAAGACAGACATGAAG
                    ************************************************************

HsCD00547111        CAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGATTATGAGCGAG
NM_001145547.1      CAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGATTATGAGCGAG
NM_032905.4         CAAGTGGGTTTGCAAGGAGACCAGATCCAGATTCTGATGAAGATGAAGATTATGAGCGAG
                    ************************************************************

HsCD00547111        AGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGA
NM_001145547.1      AGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGA
NM_032905.4         AGAGGAGGAAAAGAAGTATGGGCGGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGA
                    ************************************************************

HsCD00547111        AAGACAAAGAGTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCTCGATCACAGT
NM_001145547.1      AAGACAAAGAGTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCTCGATCACAGT
NM_032905.4         AAGACAAAGAGTTACCCCGAGATTTTCCTTATGAAGAGGACTCAAGACCTCGATCACAGT
                    ************************************************************

HsCD00547111        CTTCCAAAGCAGCCATTCCTCCCCCAGTGTACGAGGAACAAGACAGACCGAGATCTCCAA
NM_001145547.1      CTTCCAAAGCAGCCATTCCTCCCCCAGTGTACGAGGAACAAGACAGACCGAGATCTCCAA
NM_032905.4         CTTCCAAAGCAGCCATTCCTCCCCCAGTGTACGAGGAACAAGACAGACCGAGATCTCCAA
                    ************************************************************

HsCD00547111        CCGGACCTAGCAACTCCTTCCTCGCTAACATGGGGGGCACGGTGGCGCACAAGATCATGC
NM_001145547.1      CCGGACCTAGCAACTCCTTCCTCGCTAACATGGGGGGCACGGTGGCGCACAAGATCATGC
NM_032905.4         CCGGACCTAGCAACTCCTTCCTCGCTAACATGGGGGGCACGGTGGCGCACAAGATCATGC
                    ************************************************************

HsCD00547111        AGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCATGAGCAGGGCCTGAGCACTG
NM_001145547.1      AGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCATGAGCAGGGCCTGAGCACTG
NM_032905.4         AGAAGTACGGCTTCCGGGAGGGCCAGGGTCTGGGGAAGCATGAGCAGGGCCTGAGCACTG
                    ************************************************************

HsCD00547111        CCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGCGACGCCACAG
NM_001145547.1      CCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGCGACGCCACAG
NM_032905.4         CCTTGTCAGTGGAGAAGACCAGCAAGCGTGGCGGCAAGATCATCGTGGGCGACGCCACAG
                    ************************************************************

HsCD00547111        AGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTTAAGTGTCCTA
NM_001145547.1      AGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTTAAGTGTCCTA
NM_032905.4         AGAAAGATGCATCCAAGAAGTCAGATTCAAATCCGCTGACTGAAATACTTAAGTGTCCTA
                    ************************************************************

HsCD00547111        CTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGGGAGGTGGATGAAGACTTGGAAG
NM_001145547.1      CTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGAGAGGTGGATGAAGACTTGGAAG
NM_032905.4         CTAAAGTGGTCTTACTAAGGAACATGGTTGGTGCGGGAGAGGTGGATGAAGACTTGGAAG
                    *************************************.**********************

HsCD00547111        TTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTGATATTTGAAA
NM_001145547.1      TTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTGATATTTGAAA
NM_032905.4         TTGAAACCAAGGAAGAATGTGAAAAATATGGCAAAGTTGGAAAATGTGTGATATTTGAAA
                    ************************************************************

HsCD00547111        TTCCTGGTGCCCCTGATGATGAAGCAGTACGGATATTTTTAGAATTTGAGAGAGTTGAAT
NM_001145547.1      TTCCTGGTGCCCCTGATGATGAAGCAGTACGGATATTTTTAGAATTTGAGAGAGTTGAAT
NM_032905.4         TTCCTGGTGCCCCTGATGATGAAGCAGTACGGATATTTTTAGAATTTGAGAGAGTTGAAT
                    ************************************************************

HsCD00547111        CAGCAATTAAAGCGGTTGTTGACTTGAATGGGAGGTATTTTGGTGGACGGGTGGTAAAAG
NM_001145547.1      CAGCAATTAAAGCGGTTGTTGACTTGAATGGGAGGTATTTTGGTGGACGGGTGGTAAAAG
NM_032905.4         CAGCAATTAAAGCGGTTGTTGACTTGAATGGGAGGTATTTTGGTGGACGGGTGGTAAAAG
                    ************************************************************

HsCD00547111        CATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTTGGCAGAACAAGTTTAACGCG
NM_001145547.1      CATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTTGGCAGAACAAGTTTGA----
NM_032905.4         CATGTTTCTACAATTTGGACAAATTCAGGGTCTTGGATTTGGCAGAACAAGTTTGA----
                    ******************************************************.*    

HsCD00547111        ACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG
NM_001145547.1      -----------------------------------------------
NM_032905.4         -----------------------------------------------
                                                                   

Pathways

WikiPathway : mRNA processing

Protein

SMART domain : G_patch : glycine rich nucleic binding domain
SMART domain : RRM_1 : RNA recognition motif
Structural Annotation Wiki : 421608
HPRD : 09501

Clone

Cloning Information : 623358
Original Clone ID : 85785

Experimental

TargetTrack - Experimental data : 421608

Organism


TAX_ID : 9606
Species Specific ID: 84991

Genome

Chromosome : 10
Map Location : 10p15.1
Ensembl : ENSG00000134453

Reagents

Labome : SPF45-antibody