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Detailed Vector Information: pET32-DEST

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pET32-DEST              
Source: Kelley Moremen
Description: Bacterial expression vector with T7 promoter and N-terminal Trx, 6xHis and S-tags; Thrombin and enterokinase cleavage sites; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: Empty vector contains ccdB death cassette and Chloramphenicol resistance genes. Empty vector must be propagated in DB3.1 bacterial cells.
Publications: None
Authors: University of Georgia
Kelley Moremen
EvNO00548204

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No restriction
Special MTA: SPTA

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pET32-DEST

pET32-DEST in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and N-terminal Trx, 6xHis and S-tags; Thrombin and enterokinase cleavage sites; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: None
Size (bp): 7611
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: University of Georgia
Kelley Moremen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
death cassette ccdB ccdB death cassette (removed when gene insert is present) 5931 7236
promoter T7 T7 promoter 5120 5136
protease cleavage site Thrombin Thrombin cleavage site 5583 5600
protease cleavage site Enterokinase Enterokinase cleavage site 5667 5681
recombination site attR1 recombination site 5693 5821
recombination site attR2 recombination site 7277 7401
repressor protein gene LacI Lac repressor 3647 4606
selectable marker AmpR ampicillin resistance gene 598 1458
selectable marker CmR chloramphenicol resistance gene (removed when gene insert is present) 5930 6589
tag TRX N-terminal TRX tag 5208 5534
tag S-tag N-terminal S tag 5535 5552
tag His N-terminal 6xHis tag 5556 5573
trxn regulatory element lac operator Lac operator 5139 5163
trxn termination sequence T7 term T7 terminator 7525 7586

 

Species Specific ID: None
Target GenBank: None