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DCTN3 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  DCTN3
Gene Name:  dynactin 3 (p22)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH130862.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00005496

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 561nts         Open reading frame : 1 to 561
Reference Sequence Alignment

ATGGCGGGTCTGACTGACTTGCAGCGGCTACAGGCCCGAGTGGAAGAGCTGGAGCGCTGG
GTGTACGGGCCGGGCGGGGCGCGCGGCTCACGGAAGGTGGCTGACGGCCTGGTCAAGGTG
CAGGTGGCTTTGGGGAACATTTCCAGCAAGAGGGAGAGGGTGAAGATTCTCTACAAAAAG
ATTGAAGATCTGATCAAGTACCTGGATCCTGAGTACATCGACCGCATTGCCATACCTGAT
GCCTCTAAGCTGCAATTCATCCTAGCAGAGGAGCAGTTTATCCTTTCCCAGGTTGCACTC
CTGGAGCAGGTGAATGCCTTGGTGCCCATGCTGGACAGTGCTCACATCAAAGCCGTTCCT
GAGCATGCTGCCCGCCTGCAGCGCTTGGCCCAGATCCACATTCAGCAGCAGGACCAGTGT
GTGGAAATCACTGAGGAGTCCAAGGCTCTCCTGGAGGAATACAACAAGACTACAATGCTT
CTCTCCAAGCAATTCGTGCAGTGGGATGAGCTACTTTGCCAGCTAGAGGCCGCCACGCAA
GTGAAGCCAGCAGAGGAGGAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 596
Start on reference sequence 36
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5’ tcgcgttaacgctagcatggatctc 3’ 300 324
primer site reverse primer recommended reverse primer site 5’ gtaacatcagagattttgagacac 3’ 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DCTN3
Symbol Nomenclature : DCTN3
SYNONYM : DCTN-22; DCTN22
Designation : dynactin complex subunit 22 kDa subunit|dynactin light chain|dynactin subunit 3
Full Nomenclature : dynactin 3 (p22)
GENEID : 11258
Locus Tag : RP11-296L22.3
GI : 60834014
GenBank Accession : AY894037
HGNC : 2713
MIM : 607387
Vega : OTTHUMG00000019830
Target GenBank: NM_007234

 

Reference Sequence Alignment

  

HsCD00005496        ATGGCGGGTCTGACTGACTTGCAGCGGCTACAGGCCCGAGTGGAAGAGCTGGAGCGCTGG
NM_001281427.1      ATGGCGGGTCTGACTGACTTGCAGCGGCTACAGGCCCGAGTGGAAGAGCTGGAGCGCTGG
NM_007234.4         ATGGCGGGTCTGACTGACTTGCAGCGGCTACAGGCCCGAGTGGAAGAGCTGGAGCGCTGG
                    ************************************************************

HsCD00005496        GTGTACGGGCCGGGCGGGGCGCGCGGCTCACGGAAGGTGGCTGACGGCCTGGTCAAGGTG
NM_001281427.1      GTGTACGGGCCGGGCGGGGCGCGCGGCTCACGGAAGGTGGCTGACGGCCTGGTCAAGGTG
NM_007234.4         GTGTACGGGCCGGGCGGGGCGCGCGGCTCACGGAAGGTGGCTGACGGCCTGGTCAAGGTG
                    ************************************************************

HsCD00005496        CAGGTGGCTTTGGGGAACATTTCCAGCAAGAGGGAGAGGGTGAAGATTCTCTACAAAAAG
NM_001281427.1      CAGGTGGCTTTGGGGAACATTTCCAGCAAGAGGGAGAGGGTGAAGATTCTCTACAAAAAG
NM_007234.4         CAGGTGGCTTTGGGGAACATTTCCAGCAAGAGGGAGAGGGTGAAGATTCTCTACAAAAAG
                    ************************************************************

HsCD00005496        ATTGAAGATCTGATCAAGTACCTGGATCCTGAGTACATCGACCGCATTGCCATACCTGAT
NM_001281427.1      ATTGAAGATCTGATCAAGTACCTGGATCCTGAGTACATCGACCGCATTGCCATACCTGAT
NM_007234.4         ATTGAAGATCTGATCAAGTACCTGGATCCTGAGTACATCGACCGCATTGCCATACCTGAT
                    ************************************************************

HsCD00005496        GCCTCTAAGCTGCAATTCATCCTAGCAGAGGAGCAGTTTATCCTTTCCCAGGTTGCACTC
NM_001281427.1      GCCTCTAAGCTGCAATTCATCCTAGCAGAGGAGCAGTTTATCCTTTCCCAGGTTGCACTC
NM_007234.4         GCCTCTAAGCTGCAATTCATCCTAGCAGAGGAGCAGTTTATCCTTTCCCAGGTTGCACTC
                    ************************************************************

HsCD00005496        CTGGAGCAGGTGAATGCCTTGGTGCCCATGCTGGACAGTGCTCACATCAAAGCCGTTCCT
NM_001281427.1      CTGGAGCAGGTGAATGCCTTGGTGCCCATGCTGGACAGTGCTCACATCAAAGCCGTTCCT
NM_007234.4         CTGGAGCAGGTGAATGCCTTGGTGCCCATGCTGGACAGTGCTCACATCAAAGCCGTTCCT
                    ************************************************************

HsCD00005496        GAGCATGCTGCCCGCCTGCAGCGCTTGGCCCAGATCCACATTCAGCAGCAGGACCAGTGT
NM_001281427.1      GAGCATGCTGCCCGCCTGCAGCGCTTGGCCCAGATCCACATTCAGCAGCAG---------
NM_007234.4         GAGCATGCTGCCCGCCTGCAGCGCTTGGCCCAGATCCACATTCAGCAGCAGGACCAGTGT
                    ***************************************************         

HsCD00005496        GTGGAAATCACTGAGGAGTCCAAGGCTCTCCTGGAGGAATACAACAAGACTACAATGCTT
NM_001281427.1      ---------------------ATTCCTATTCTCACCTTGACCTACAACCAGACAATGCTT
NM_007234.4         GTGGAAATCACTGAGGAGTCCAAGGCTCTCCTGGAGGAATACAACAAGACTACAATGCTT
                                         *:  **.* ** ..  :.:.*:**** .. *********

HsCD00005496        CTCTCCAAGCAATTCGTGCAGTGGGATGAGCTACTTTGCCAGCTAGAGGCCGCCACGCAA
NM_001281427.1      CTCTCCAAGCAATTCGTGCAGTGGGATGAGCTACTTTGCCAGCTAGAGGCCGCCACGCAA
NM_007234.4         CTCTCCAAGCAATTCGTGCAGTGGGATGAGCTACTTTGCCAGCTAGAGGCCGCCACGCAA
                    ************************************************************

HsCD00005496        GTGAAGCCAGCAGAGGAGGAC
NM_001281427.1      GTGAAGCCAGCAGAGGAGTGA
NM_007234.4         GTGAAGCCAGCAGAGGAGTGA
                    ****************** ..

Pathways

Reactome : Adaptive Immune System
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Centrosome maturation
Reactome : G2/M Transition
Reactome : Immune System
Reactome : Loss of Nlp from mitotic centrosomes
Reactome : Loss of proteins required for interphase microtubule organizationÿ from the centrosome
Reactome : MHC class II antigen presentation
Reactome : Mitotic G2-G2/M phases
Reactome : Recruitment of mitotic centrosome proteins and complexes
Reactome : Regulation of PLK1 Activity at G2/M Transition

Protein

UniProt : O75935
HPRD : 06303

Clone

Cloning Information : 130862
HIP Master Clone ID : 107019
Original Clone ID : FLH130862.01L

Organism


TAX_ID : 9606
Species Specific ID: 11258

Genome

Chromosome : 9
Map Location : 9p13
Ensembl : ENSG00000137100

Reagents

Labome : DCTN3-antibody