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SIAH1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SIAH1
Gene Name:  siah E3 ubiquitin protein ligase 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH119867.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
PMID: 22675025
Title: Nuclear hormone receptor corepressor promotes esophageal cancer cell invasion by transcriptional repression of interferon-?-inducible protein 10 in a casein kinase 2-dependent manner
Authors: HIP
HsCD00005662

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 849nts         Open reading frame : 1 to 849
Reference Sequence Alignment

ATGAGCCGTCAGACTGCTACAGCATTACCTACCGGTACCTCGAAGTGTCCACCATCCCAG
AGGGTGCCTGCCCTGACTGGCACAACTGCATCCAACAATGACTTGGCGAGTCTTTTTGAG
TGTCCAGTCTGCTTTGACTATGTGTTACCGCCCATTCTTCAATGTCAGAGTGGCCATCTT
GTTTGTAGCAACTGTCGCCCAAAGCTCACATGTTGTCCAACTTGCCGGGGCCCTTTGGGA
TCCATTCGCAACTTGGCTATGGAGAAAGTGGCTAATTCAGTACTTTTCCCCTGTAAATAT
GCGTCTTCTGGATGTGAAATAACTCTGCCACACACAGAAAAAGCAGACCATGAAGAGCTC
TGTGAGTTTAGGCCTTATTCCTGTCCGTGCCCTGGTGCTTCCTGTAAATGGCAAGGCTCT
CTGGATGCTGTAATGCCCCATCTGATGCATCAGCATAAGTCCATTACAACCCTACAGGGA
GAGGATATAGTTTTTCTTGCTACAGACATTAATCTTCCTGGTGCTGTTGACTGGGTGATG
ATGCAGTCCTGTTTTGGCTTTCACTTCATGTTAGTCTTAGAGAAACAGGAAAAATACGAT
GGTCACCAGCAGTTCTTCGCAATCGTACAGCTGATAGGAACACGCAAGCAAGCTGAAAAT
TTTGCTTACCGACTTGAGCTAAATGGTCATAGGCGACGATTGACTTGGGAAGCGACTCCT
CGATCTATTCATGAAGGAATTGCAACAGCCATTATGAATAGCGACTGTCTAGTCTTTGAC
ACCAGCATTGCACAGCTTTTTGCAGAAAATGGCAATTTAGGCATCAATGTAACTATTTCC
ATGTGTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 966
Start on reference sequence 118
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SIAH1
Symbol Nomenclature : SIAH1
SYNONYM : SIAH1A
Designation : E3 ubiquitin-protein ligase SIAH1|seven in absentia homolog 1|siah-1a
Full Nomenclature : siah E3 ubiquitin protein ligase 1
GENEID : 6477
GI : 61364080
GenBank Accession : AY890546
HGNC : 10857
MIM : 602212
Vega : OTTHUMG00000175417
Target GenBank: BC035562

 

Reference Sequence Alignment

  

HsCD00005662        ------------------------------------------------------------
NM_001006610.1      ATGACGGGAAAGGCTACTCCACCTTCTCTGTACTCCTGGAGGGGAGTCTTGTTCACATGT
NM_003031.3         ------------------------------------------------------------
                                                                                

HsCD00005662        ---------------------------------ATGAGCCGTCAGACTGCTACAGCATTA
NM_001006610.1      TTACCAGCGGCCAGGACAAGGAAGAGAAAAGAAATGAGCCGTCAGACTGCTACAGCATTA
NM_003031.3         ---------------------------------ATGAGCCGTCAGACTGCTACAGCATTA
                                                     ***************************

HsCD00005662        CCTACCGGTACCTCGAAGTGTCCACCATCCCAGAGGGTGCCTGCCCTGACTGGCACAACT
NM_001006610.1      CCTACCGGTACCTCGAAGTGTCCACCATCCCAGAGGGTGCCTGCCCTGACTGGCACAACT
NM_003031.3         CCTACCGGTACCTCGAAGTGTCCACCATCCCAGAGGGTGCCTGCCCTGACTGGCACAACT
                    ************************************************************

HsCD00005662        GCATCCAACAATGACTTGGCGAGTCTTTTTGAGTGTCCAGTCTGCTTTGACTATGTGTTA
NM_001006610.1      GCATCCAACAATGACTTGGCGAGTCTTTTTGAGTGTCCAGTCTGCTTTGACTATGTGTTA
NM_003031.3         GCATCCAACAATGACTTGGCGAGTCTTTTTGAGTGTCCAGTCTGCTTTGACTATGTGTTA
                    ************************************************************

HsCD00005662        CCGCCCATTCTTCAATGTCAGAGTGGCCATCTTGTTTGTAGCAACTGTCGCCCAAAGCTC
NM_001006610.1      CCGCCCATTCTTCAATGTCAGAGTGGCCATCTTGTTTGTAGCAACTGTCGCCCAAAGCTC
NM_003031.3         CCGCCCATTCTTCAATGTCAGAGTGGCCATCTTGTTTGTAGCAACTGTCGCCCAAAGCTC
                    ************************************************************

HsCD00005662        ACATGTTGTCCAACTTGCCGGGGCCCTTTGGGATCCATTCGCAACTTGGCTATGGAGAAA
NM_001006610.1      ACATGTTGTCCAACTTGCCGGGGCCCTTTGGGATCCATTCGCAACTTGGCTATGGAGAAA
NM_003031.3         ACATGTTGTCCAACTTGCCGGGGCCCTTTGGGATCCATTCGCAACTTGGCTATGGAGAAA
                    ************************************************************

HsCD00005662        GTGGCTAATTCAGTACTTTTCCCCTGTAAATATGCGTCTTCTGGATGTGAAATAACTCTG
NM_001006610.1      GTGGCTAATTCAGTACTTTTCCCCTGTAAATATGCGTCTTCTGGATGTGAAATAACTCTG
NM_003031.3         GTGGCTAATTCAGTACTTTTCCCCTGTAAATATGCGTCTTCTGGATGTGAAATAACTCTG
                    ************************************************************

HsCD00005662        CCACACACAGAAAAAGCAGACCATGAAGAGCTCTGTGAGTTTAGGCCTTATTCCTGTCCG
NM_001006610.1      CCACACACAGAAAAAGCAGACCATGAAGAGCTCTGTGAGTTTAGGCCTTATTCCTGTCCG
NM_003031.3         CCACACACAGAAAAAGCAGACCATGAAGAGCTCTGTGAGTTTAGGCCTTATTCCTGTCCG
                    ************************************************************

HsCD00005662        TGCCCTGGTGCTTCCTGTAAATGGCAAGGCTCTCTGGATGCTGTAATGCCCCATCTGATG
NM_001006610.1      TGCCCTGGTGCTTCCTGTAAATGGCAAGGCTCTCTGGATGCTGTAATGCCCCATCTGATG
NM_003031.3         TGCCCTGGTGCTTCCTGTAAATGGCAAGGCTCTCTGGATGCTGTAATGCCCCATCTGATG
                    ************************************************************

HsCD00005662        CATCAGCATAAGTCCATTACAACCCTACAGGGAGAGGATATAGTTTTTCTTGCTACAGAC
NM_001006610.1      CATCAGCATAAGTCCATTACAACCCTACAGGGAGAGGATATAGTTTTTCTTGCTACAGAC
NM_003031.3         CATCAGCATAAGTCCATTACAACCCTACAGGGAGAGGATATAGTTTTTCTTGCTACAGAC
                    ************************************************************

HsCD00005662        ATTAATCTTCCTGGTGCTGTTGACTGGGTGATGATGCAGTCCTGTTTTGGCTTTCACTTC
NM_001006610.1      ATTAATCTTCCTGGTGCTGTTGACTGGGTGATGATGCAGTCCTGTTTTGGCTTTCACTTC
NM_003031.3         ATTAATCTTCCTGGTGCTGTTGACTGGGTGATGATGCAGTCCTGTTTTGGCTTTCACTTC
                    ************************************************************

HsCD00005662        ATGTTAGTCTTAGAGAAACAGGAAAAATACGATGGTCACCAGCAGTTCTTCGCAATCGTA
NM_001006610.1      ATGTTAGTCTTAGAGAAACAGGAAAAATACGATGGTCACCAGCAGTTCTTCGCAATCGTA
NM_003031.3         ATGTTAGTCTTAGAGAAACAGGAAAAATACGATGGTCACCAGCAGTTCTTCGCAATCGTA
                    ************************************************************

HsCD00005662        CAGCTGATAGGAACACGCAAGCAAGCTGAAAATTTTGCTTACCGACTTGAGCTAAATGGT
NM_001006610.1      CAGCTGATAGGAACACGCAAGCAAGCTGAAAATTTTGCTTACCGACTTGAGCTAAATGGT
NM_003031.3         CAGCTGATAGGAACACGCAAGCAAGCTGAAAATTTTGCTTACCGACTTGAGCTAAATGGT
                    ************************************************************

HsCD00005662        CATAGGCGACGATTGACTTGGGAAGCGACTCCTCGATCTATTCATGAAGGAATTGCAACA
NM_001006610.1      CATAGGCGACGATTGACTTGGGAAGCGACTCCTCGATCTATTCATGAAGGAATTGCAACA
NM_003031.3         CATAGGCGACGATTGACTTGGGAAGCGACTCCTCGATCTATTCATGAAGGAATTGCAACA
                    ************************************************************

HsCD00005662        GCCATTATGAATAGCGACTGTCTAGTCTTTGACACCAGCATTGCACAGCTTTTTGCAGAA
NM_001006610.1      GCCATTATGAATAGCGACTGTCTAGTCTTTGACACCAGCATTGCACAGCTTTTTGCAGAA
NM_003031.3         GCCATTATGAATAGCGACTGTCTAGTCTTTGACACCAGCATTGCACAGCTTTTTGCAGAA
                    ************************************************************

HsCD00005662        AATGGCAATTTAGGCATCAATGTAACTATTTCCATGTGTTAG
NM_001006610.1      AATGGCAATTTAGGCATCAATGTAACTATTTCCATGTGTTGA
NM_003031.3         AATGGCAATTTAGGCATCAATGTAACTATTTCCATGTGTTGA
                    ****************************************..

Pathways

Panther : Wnt signaling pathway
Panther : p53 pathway
Panther : p53 pathway feedback loops 2
Reactome : Axon guidance
Reactome : Developmental Biology
Reactome : Netrin-1 signaling

Protein

UniProt : Q8IUQ4
HPRD : 03736

Clone

Cloning Information : 119867
HIP Master Clone ID : 95521
Original Clone ID : FLH119867.01X

Organism


TAX_ID : 9606
Species Specific ID: 6477

Genome

Chromosome : 16
Map Location : 16q12.1
Ensembl : ENSG00000196470

Reagents

Labome : SIAH1-antibody