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CA7 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CA7
Gene Name:  carbonic anhydrase VII
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH119836.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00005671

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 795nts         Open reading frame : 1 to 795
Reference Sequence Alignment

ATGACCGGCCACCACGGCTGGGGCTACGGCCAGGACGACGGCCCCTCGCATTGGCACAAG
CTGTATCCCATTGCCCAGGGAGATCGCCAATCACCCATCAATATCATCTCCAGCCAGGCT
GTGTACTCTCCCAGCCTGCAACCACTGGAGCTTTCCTATGAGGCCTGCATGTCCCTCAGC
ATCACCAACAATGGCCACTCTGTCCAGGTAGACTTCAATGACAGCGATGACCGAACCGTG
GTGACTGGGGGCCCCCTGGAAGGGCCCTACCGCCTCAAGCAGTTTCACTTCCACTGGGGC
AAGAAGCACGATGTGGGTTCTGAGCACACGGTGGACGGCAAGTCCTTCCCCAGCGAGCTG
CATCTGGTTCACTGGAATGCCAAGAAGTACAGCACTTTTGGGGAGGCGGCCTCAGCACCT
GATGGCCTGGCTGTGGTTGGTGTTTTTTTGGAGACAGGAGACGAGCACCCCAGCATGAAT
CGTCTGACAGATGCGCTCTACATGGTCCGGTTCAAGGGCACCAAAGCCCAGTTCAGCTGC
TTCAACCCCAAGTGCCTCCTGCCTGCCAGCCGGCACTACTGGACCTACCCGGGCTCTCTG
ACGACTCCCCCACTCAGTGAGAGTGTCACCTGGATTGTGCTCCGGGAGCCCATCTGCATC
TCTGAAAGGCAGATGGGGAAGTTCCGGAGCCTGCTTTTTACCTCGGAGGACGATGAGAGG
ATCCACATGGTGAACAACTTCCGGCCACCACAGCCACTGAAGGGCCGCGTGGTAAAGGCC
TCCTTCCGGGCGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 860
Start on reference sequence 66
Mutations:
None
Discrepancy:
c792g
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CA7
Symbol Nomenclature : CA7
SYNONYM : CAVII
Designation : CA-VII|carbonate dehydratase VII|carbonic anhydrase 7|carbonic dehydratase VII
Full Nomenclature : carbonic anhydrase VII
GENEID : 766
GI : 60654490
GenBank Accession : AY893019
HGNC : 1381
MIM : 114770
Vega : OTTHUMG00000137524
Target GenBank: BC033865

 

Reference Sequence Alignment

  

HsCD00005671      ATGACCGGCCACCACGGCTGGGGCTACGGCCAGGACGACGGCCCCTCGCATTGGCACAAG
NM_005182.2       ATGACCGGCCACCACGGCTGGGGCTACGGCCAGGACGACGGCCCCTCGCATTGGCACAAG
                  ************************************************************

HsCD00005671      CTGTATCCCATTGCCCAGGGAGATCGCCAATCACCCATCAATATCATCTCCAGCCAGGCT
NM_005182.2       CTGTATCCCATTGCCCAGGGAGATCGCCAATCACCCATCAATATCATCTCCAGCCAGGCT
                  ************************************************************

HsCD00005671      GTGTACTCTCCCAGCCTGCAACCACTGGAGCTTTCCTATGAGGCCTGCATGTCCCTCAGC
NM_005182.2       GTGTACTCTCCCAGCCTGCAACCACTGGAGCTTTCCTATGAGGCCTGCATGTCCCTCAGC
                  ************************************************************

HsCD00005671      ATCACCAACAATGGCCACTCTGTCCAGGTAGACTTCAATGACAGCGATGACCGAACCGTG
NM_005182.2       ATCACCAACAATGGCCACTCTGTCCAGGTAGACTTCAATGACAGCGATGACCGAACCGTG
                  ************************************************************

HsCD00005671      GTGACTGGGGGCCCCCTGGAAGGGCCCTACCGCCTCAAGCAGTTTCACTTCCACTGGGGC
NM_005182.2       GTGACTGGGGGCCCCCTGGAAGGGCCCTACCGCCTCAAGCAGTTTCACTTCCACTGGGGC
                  ************************************************************

HsCD00005671      AAGAAGCACGATGTGGGTTCTGAGCACACGGTGGACGGCAAGTCCTTCCCCAGCGAGCTG
NM_005182.2       AAGAAGCACGATGTGGGTTCTGAGCACACGGTGGACGGCAAGTCCTTCCCCAGCGAGCTG
                  ************************************************************

HsCD00005671      CATCTGGTTCACTGGAATGCCAAGAAGTACAGCACTTTTGGGGAGGCGGCCTCAGCACCT
NM_005182.2       CATCTGGTTCACTGGAATGCCAAGAAGTACAGCACTTTTGGGGAGGCGGCCTCAGCACCT
                  ************************************************************

HsCD00005671      GATGGCCTGGCTGTGGTTGGTGTTTTTTTGGAGACAGGAGACGAGCACCCCAGCATGAAT
NM_005182.2       GATGGCCTGGCTGTGGTTGGTGTTTTTTTGGAGACAGGAGACGAGCACCCCAGCATGAAT
                  ************************************************************

HsCD00005671      CGTCTGACAGATGCGCTCTACATGGTCCGGTTCAAGGGCACCAAAGCCCAGTTCAGCTGC
NM_005182.2       CGTCTGACAGATGCGCTCTACATGGTCCGGTTCAAGGGCACCAAAGCCCAGTTCAGCTGC
                  ************************************************************

HsCD00005671      TTCAACCCCAAGTGCCTCCTGCCTGCCAGCCGGCACTACTGGACCTACCCGGGCTCTCTG
NM_005182.2       TTCAACCCCAAGTGCCTCCTGCCTGCCAGCCGGCACTACTGGACCTACCCGGGCTCTCTG
                  ************************************************************

HsCD00005671      ACGACTCCCCCACTCAGTGAGAGTGTCACCTGGATTGTGCTCCGGGAGCCCATCTGCATC
NM_005182.2       ACGACTCCCCCACTCAGTGAGAGTGTCACCTGGATTGTGCTCCGGGAGCCCATCTGCATC
                  ************************************************************

HsCD00005671      TCTGAAAGGCAGATGGGGAAGTTCCGGAGCCTGCTTTTTACCTCGGAGGACGATGAGAGG
NM_005182.2       TCTGAAAGGCAGATGGGGAAGTTCCGGAGCCTGCTTTTTACCTCGGAGGACGATGAGAGG
                  ************************************************************

HsCD00005671      ATCCACATGGTGAACAACTTCCGGCCACCACAGCCACTGAAGGGCCGCGTGGTAAAGGCC
NM_005182.2       ATCCACATGGTGAACAACTTCCGGCCACCACAGCCACTGAAGGGCCGCGTGGTAAAGGCC
                  ************************************************************

HsCD00005671      TCCTTCCGGGCGTTG
NM_005182.2       TCCTTCCGGGCCTGA
                  *********** * .

Pathways

Reactome : Metabolism
Reactome : Reversible hydration of carbon dioxide

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
UniProt : Q86YU0
UniProt : P43166
HPRD : 00263

Clone

Cloning Information : 119836
HIP Master Clone ID : 95490
Original Clone ID : FLH119836.01L

Organism


TAX_ID : 9606
Species Specific ID: 766

Genome

Chromosome : 16
Map Location : 16q22.1
Ensembl : ENSG00000168748

Reagents

Labome : CA7-antibody