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HYAL1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HYAL1
Gene Name:  hyaluronoglucosaminidase 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH139846.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00005732

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1308nts         Open reading frame : 1 to 1308
Reference Sequence Alignment

ATGGCAGCTCACCTGCTTCCCATCTGCGCCCTCTTCCTGACCTTACTCGATATGGCCCAA
GGCTTTAGGGGCCCCTTGCTACCCAACCGGCCCTTCACCACCGTCTGGAATGCAAACACC
CAGTGGTGCCTGGAGAGGCACGGTGTGGACGTGGATGTCAGTGTCTTCGATGTGGTAGCC
AACCCAGGGCAGACCTTCCGCGGCCCTGACATGACAATTTTCTATAGCTCCCAGCTGGGC
ACCTACCCCTACTACACGCCCACTGGGGAGCCTGTGTTTGGTGGTCTGCCCCAGAATGCC
AGCCTGATTGCCCACCTGGCCCGCACATTCCAGGACATCCTGGCTGCCATACCTGCTCCT
GACTTCTCAGGGCTGGCAGTCATCGACTGGGAGGCATGGCGCCCACGCTGGGCCTTCAAC
TGGGACACCAAGGACATTTACCGGCAGCGCTCACGGGCACTGGTACAGGCACAGCACCCT
GATTGGCCAGCTCCTCAGGTGGAGGCAGTAGCCCAGGACCAGTTCCAGGGAGCTGCACGG
GCCTGGATGGCAGGCACCCTCCAGCTGGGGCGGGCACTGCGTCCTCGCGGCCTCTGGGGC
TTCTATGGCTTCCCTGACTGCTACAACTATGACTTTCTAAGCCCCAACTACACCGGCCAG
TGCCCATCAGGCATCCGTGCCCAAAATGACCAGCTAGGGTGGCTGTGGGGCCAGAGCCGT
GCCCTCTATCCCAGCATCTACATGCCCGCAGTGCTGGAGGGCACAGGGAAGTCACAGATG
TATGTGCAACACCGTGTGGCCGAGGCATTCCGTGTGGCTGTGGCTGCTGGTGACCCCAAT
CTGCCGGTGCTGCCCTATGTCCAGATCTTCTATGACACGACAAACCACTTTCTGCCCCTG
GATGAGCTGGAGCACAGCCTGGGGGAGAGTGCGGCCCAGGGGGCAGCTGGAGTGGTGCTC
TGGGTGAGCTGGGAAAATACAAGAACCAAGGAATCATGTCAGGCCATCAAGGAGTATATG
GACACTACACTGGGGCCCTTCATCCTGAACGTGACCAGTGGGGCCCTTCTCTGCAGTCAA
GCCCTGTGCTCCGGCCATGGCCGCTGTGTCCGCCGCACCAGCCACCCCAAAGCCCTCCTC
CTCCTTAACCCTGCCAGTTTCTCCATCCAGCTCACGCCTGGTGGTGGGCCCCTGAGCCTG
CGGGGTGCCCTCTCACTTGAAGATCAGGCACAGATGGCTGTGGAGTTCAAATGTCGATGC
TACCCTGGCTGGCAGGCACCGTGGTGTGAGCGGAAGAGCATGTGGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1422
Start on reference sequence 115
Mutations:
None
Discrepancy:
c9t
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HYAL1
Symbol Nomenclature : HYAL1
SYNONYM : HYAL-1; LUCA1; NAT6
Designation : hyaluronidase-1|luCa-1|lung carcinoma protein 1|plasma hyaluronidase|tumor suppressor LUCA-1
Full Nomenclature : hyaluronoglucosaminidase 1
GENEID : 3373
GI : 61364299
GenBank Accession : AY890579
HGNC : 5320
MIM : 607071
Vega : OTTHUMG00000156941
Target GenBank: BC035695

 

Reference Sequence Alignment

  

HsCD00005732      ATGGCAGCTCACCTGCTTCCCATCTGCGCCCTCTTCCTGACCTTACTCGATATGGCCCAA
NM_153281.1       ATGGCAGCCCACCTGCTTCCCATCTGCGCCCTCTTCCTGACCTTACTCGATATGGCCCAA
NM_033159.3       ATGGCAGCCCACCTGCTTCCCATCTGCGCCCTCTTCCTGACCTTACTCGATATGGCCCAA
NM_153282.2       ATGGCAGCCCACCTGCTTCCCATCTGCGCCCTCTTCCTGACCTTACTCGATATGGCCCAA
                  ******** ***************************************************

HsCD00005732      GGCTTTAGGGGCCCCTTGCTACCCAACCGGCCCTTCACCACCGTCTGGAATGCAAACACC
NM_153281.1       GGCTTTAGGGGCCCCTTGCTACCCAACCGGCCCTTCACCACCGTCTGGAATGCAAACACC
NM_033159.3       GGCTTTAGGGGCCCCTTGCTACCCAACCGGCCCTTCACCACCGTCTGGAATGCAAACACC
NM_153282.2       GGCTTTAGGGGCCCCTTGCTACCCAACCGGCCCTTCACCACCGTCTGGAATGCAAACACC
                  ************************************************************

HsCD00005732      CAGTGGTGCCTGGAGAGGCACGGTGTGGACGTGGATGTCAGTGTCTTCGATGTGGTAGCC
NM_153281.1       CAGTGGTGCCTGGAGAGGCACGGTGTGGACGTGGATGTCAGTGTCTTCGATGTGGTAGCC
NM_033159.3       CAGTGGTGCCTGGAGAGGCACGGTGTGGACGTGGATGTCAGTGTCTTCGATGTGGTAGCC
NM_153282.2       CAGTGGTGCCTGGAGAGGCACGGTGTGGACGTGGATGTCAGTGTCTTCGATGTGGTAGCC
                  ************************************************************

HsCD00005732      AACCCAGGGCAGACCTTCCGCGGCCCTGACATGACAATTTTCTATAGCTCCCAGCTGGGC
NM_153281.1       AACCCAGGGCAGACCTTCCGCGGCCCTGACATGACAATTTTCTATAGCTCCCAGCTGGGC
NM_033159.3       AACCCAGGGCAGACCTTCCGCGGCCCTGACATGACAATTTTCTATAGCTCCCAGCTGGGC
NM_153282.2       AACCCAGGGCAGACCTTCCGCGGCCCTGACATGACAATTTTCTATAGCTCCCAGCTGGGC
                  ************************************************************

HsCD00005732      ACCTACCCCTACTACACGCCCACTGGGGAGCCTGTGTTTGGTGGTCTGCCCCAGAATGCC
NM_153281.1       ACCTACCCCTACTACACGCCCACTGGGGAGCCTGTGTTTGGTGGTCTGCCCCAGAATGCC
NM_033159.3       ACCTACCCCTACTACACGCCCACTGGGGAGCCTGTGTTTGGTGGTCTGCCCCAGAATGCC
NM_153282.2       ACCTACCCCTACTACACGCCCACTGGGGAGCCTGTGTTTGGTGGTCTGCCCCAGAATGCC
                  ************************************************************

HsCD00005732      AGCCTGATTGCCCACCTGGCCCGCACATTCCAGGACATCCTGGCTGCCATACCTGCTCCT
NM_153281.1       AGCCTGATTGCCCACCTGGCCCGCACATTCCAGGACATCCTGGCTGCCATACCTGCTCCT
NM_033159.3       AGCCTGATTGCCCACCTGGCCCGCACATTCCAGGACATCCTGGCTGCCATACCTGCTCCT
NM_153282.2       AGCCTGATTGCCCACCTGGCCCGCACATTCCAGGACATCCTGGCTGCCATACCTGCTCCT
                  ************************************************************

HsCD00005732      GACTTCTCAGGGCTGGCAGTCATCGACTGGGAGGCATGGCGCCCACGCTGGGCCTTCAAC
NM_153281.1       GACTTCTCAGGGCTGGCAGTCATCGACTGGGAGGCATGGCGCCCACGCTGGGCCTTCAAC
NM_033159.3       GACTTCTCAGGGCTGGCAGTCATCGACTGGGAGGCATGGCGCCCACGCTGGGCCTTCAAC
NM_153282.2       GACTTCTCAGGGCTGGCAGTCATCGACTGGGAGGCATGGCGCCCACGCTGGGCCTTCAAC
                  ************************************************************

HsCD00005732      TGGGACACCAAGGACATTTACCGGCAGCGCTCACGGGCACTGGTACAGGCACAGCACCCT
NM_153281.1       TGGGACACCAAGGACATTTACCGGCAGCGCTCACGGGCACTGGTACAGGCACAGCACCCT
NM_033159.3       TGGGACACCAAGGACATTTACCGGCAGCGCTCACGGGCACTGGTACAGGCACAGCACCCT
NM_153282.2       TGGGACACCAAGGACATTTACCGGCAGCGCTCACGGGCACTGGTACAGGCACAGCACCCT
                  ************************************************************

HsCD00005732      GATTGGCCAGCTCCTCAGGTGGAGGCAGTAGCCCAGGACCAGTTCCAGGGAGCTGCACGG
NM_153281.1       GATTGGCCAGCTCCTCAGGTGGAGGCAGTAGCCCAGGACCAGTTCCAGGGAGCTGCACGG
NM_033159.3       GATTGGCCAGCTCCTCAGGTGGAGGCAGTAGCCCAGGACCAGTTCCAGGGAGCTGCACGG
NM_153282.2       GATTGGCCAGCTCCTCAGGTGGAGGCAGTAGCCCAGGACCAGTTCCAGGGAGCTGCACGG
                  ************************************************************

HsCD00005732      GCCTGGATGGCAGGCACCCTCCAGCTGGGGCGGGCACTGCGTCCTCGCGGCCTCTGGGGC
NM_153281.1       GCCTGGATGGCAGGCACCCTCCAGCTGGGGCGGGCACTGCGTCCTCGCGGCCTCTGGGGC
NM_033159.3       GCCTGGATGGCAGGCACCCTCCAGCTGGGGCGGGCACTGCGTCCTCGCGGCCTCTGGGGC
NM_153282.2       GCCTGGATGGCAGGCACCCTCCAGCTGGGGCGGGCACTGCGTCCTCGCGGCCTCTGGGGC
                  ************************************************************

HsCD00005732      TTCTATGGCTTCCCTGACTGCTACAACTATGACTTTCTAAGCCCCAACTACACCGGCCAG
NM_153281.1       TTCTATGGCTTCCCTGACTGCTACAACTATGACTTTCTAAGCCCCAACTACACCGGCCAG
NM_033159.3       TTCTATGGCTTCCCTGACTGCTACAACTATGACTTTCTAAGCCCCAACTACACCGGCCAG
NM_153282.2       TTCTATGGCTTCCCTGACTGCTACAACTATGACTTTCTAAGCCCCAACTACACCGGCCAG
                  ************************************************************

HsCD00005732      TGCCCATCAGGCATCCGTGCCCAAAATGACCAGCTAGGGTGGCTGTGGGGCCAGAGCCGT
NM_153281.1       TGCCCATCAGGCATCCGTGCCCAAAATGACCAGCTAGGGTGGCTGTGGGGCCAGAGCCGT
NM_033159.3       TGCCCATCAGGCATCCGTGCCCAAAATGACCAGCTAGGGTGGCTGTGGGGCCAGAGCCGT
NM_153282.2       TGCCCATCAGGCATCCGTGCCCAAAATGACCAGCTAGGGTGGCTGTGGGGCCAGAGCCGT
                  ************************************************************

HsCD00005732      GCCCTCTATCCCAGCATCTACATGCCCGCAGTGCTGGAGGGCACAGGGAAGTCACAGATG
NM_153281.1       GCCCTCTATCCCAGCATCTACATGCCCGCAGTGCTGGAGGGCACAGGGAAGTCACAGATG
NM_033159.3       GCCCTCTATCCCAGCATCTACATGCCCGCAGTGCTGGAGGGCACAGGGAAGTCACAGATG
NM_153282.2       GCCCTCTATCCCAGCATCTACATGCCCGCAGTGCTGGAGGGCACAGGGAAGTCACAGATG
                  ************************************************************

HsCD00005732      TATGTGCAACACCGTGTGGCCGAGGCATTCCGTGTGGCTGTGGCTGCTGGTGACCCCAAT
NM_153281.1       TATGTGCAACACCGTGTGGCCGAGGCATTCCGTGTGGCTGTGGCTGCTGGTGACCCCAAT
NM_033159.3       TATGTGCAACACCGTGTGGCCGAGGCATTCCGTGTGGCTGTGGCTGCTGGTGACCCCAAT
NM_153282.2       TATGTGCAACACCGTGTGGCCGAGGCATTCCGTGTGGCTGTGGCTGCTGGTGACCCCAAT
                  ************************************************************

HsCD00005732      CTGCCGGTGCTGCCCTATGTCCAGATCTTCTATGACACGACAAACCACTTTCTGCCCCTG
NM_153281.1       CTGCCGGTGCTGCCCTATGTCCAGATCTTCTATGACACGACAAACCACTTTCTGCCCCTG
NM_033159.3       CTGCCGGTGCTGCCCTATGTCCAGATCTTCTATGACACGACAAACCACTTTCTGCCCCTG
NM_153282.2       CTGCCGGTGCTGCCCTATGTCCAGATCTTCTATGACACGACAAACCACTTTCTGCCCCTG
                  ************************************************************

HsCD00005732      GATGAGCTGGAGCACAGCCTGGGGGAGAGTGCGGCCCAGGGGGCAGCTGGAGTGGTGCTC
NM_153281.1       GATGAGCTGGAGCACAGCCTGGGGGAGAGTGCGGCCCAGGGGGCAGCTGGAGTGGTGCTC
NM_033159.3       GATGAGCTGGAGCACAGCCTGGGGGAGAGTGCGGCCCAGGGGGCAGCTGGAGTGGTGCTC
NM_153282.2       ------------------------------------------------------------
                                                                              

HsCD00005732      TGGGTGAGCTGGGAAAATACAAGAACCAAGGAATCATGTCAGGCCATCAAGGAGTATATG
NM_153281.1       TGGGTGAGCTGGGAAAATACAAGAACCAAGGAATCATGTCAGGCCATCAAGGAGTATATG
NM_033159.3       TGGGTGAGCTGGGAAAATACAAGAACCAAGGAATCATGTCAGGCCATCAAGGAGTATATG
NM_153282.2       ------------------------------GAATCATGTCAGGCCATCAAGGAGTATATG
                                                ******************************

HsCD00005732      GACACTACACTGGGGCCCTTCATCCTGAACGTGACCAGTGGGGCCCTTCTCTGCAGTCAA
NM_153281.1       GACACTACACTGGGGCCCTTCATCCTGAACGTGACCAGTGGGGCCCTTCTCTGCAGTCAA
NM_033159.3       GACACTACACTGGGGCCCTTCATCCTGAACGTGACCAGTGGGGCCCTTCTCTGCAGTCAA
NM_153282.2       GACACTACACTGGGGCCCTTCATCCTGAACGTGACCAGTGGGGCCCTTCTCTGCAGTCAA
                  ************************************************************

HsCD00005732      GCCCTGTGCTCCGGCCATGGCCGCTGTGTCCGCCGCACCAGCCACCCCAAAGCCCTCCTC
NM_153281.1       GCCCTGTGCTCCGGCCATGGCCGCTGTGTCCGCCGCACCAGCCACCCCAAAGCCCTCCTC
NM_033159.3       GCCCTGTGCTCCGGCCATGGCCGCTGTGTCCGCCGCACCAGCCACCCCAAAGCCCTCCTC
NM_153282.2       GCCCTGTGCTCCGGCCATGGCCGCTGTGTCCGCCGCACCAGCCACCCCAAAGCCCTCCTC
                  ************************************************************

HsCD00005732      CTCCTTAACCCTGCCAGTTTCTCCATCCAGCTCACGCCTGGTGGTGGGCCCCTGAGCCTG
NM_153281.1       CTCCTTAACCCTGCCAGTTTCTCCATCCAGCTCACGCCTGGTGGTGGGCCCCTGAGCCTG
NM_033159.3       CTCCTTAACCCTGCCAGTTTCTCCATCCAGCTCACGCCTGGTGGTGGGCCCCTGAGCCTG
NM_153282.2       CTCCTTAACCCTGCCAGTTTCTCCATCCAGCTCACGCCTGGTGGTGGGCCCCTGAGCCTG
                  ************************************************************

HsCD00005732      CGGGGTGCCCTCTCACTTGAAGATCAGGCACAGATGGCTGTGGAGTTCAAATGTCGATGC
NM_153281.1       CGGGGTGCCCTCTCACTTGAAGATCAGGCACAGATGGCTGTGGAGTTCAAATGTCGATGC
NM_033159.3       CGGGGTGCCCTCTCACTTGAAGATCAGGCACAGATGGCTGTGGAGTTCAAATGTCGATGC
NM_153282.2       CGGGGTGCCCTCTCACTTGAAGATCAGGCACAGATGGCTGTGGAGTTCAAATGTCGATGC
                  ************************************************************

HsCD00005732      TACCCTGGCTGGCAGGCACCGTGGTGTGAGCGGAAGAGCATGTGGTAG
NM_153281.1       TACCCTGGCTGGCAGGCACCGTGGTGTGAGCGGAAGAGCATGTGGTGA
NM_033159.3       TACCCTGGCTGGCAGGCACCGTGGTGTGAGCGGAAGAGCATGTGGTGA
NM_153282.2       TACCCTGGCTGGCAGGCACCGTGGTGTGAGCGGAAGAGCATGTGGTGA
                  **********************************************..

Pathways

Reactome : CS/DS degradation
Reactome : Chondroitin sulfate/dermatan sulfate metabolism
Reactome : Disease
Reactome : Glycogen storage diseases
Reactome : Glycosaminoglycan metabolism
Reactome : Hyaluronan metabolism
Reactome : Hyaluronan uptake and degradation
Reactome : MPS I - Hurler syndrome
Reactome : MPS II - Hunter syndrome
Reactome : MPS IIIA - Sanfilippo syndrome A
Reactome : MPS IIIB - Sanfilippo syndrome B
Reactome : MPS IIIC - Sanfilippo syndrome C
Reactome : MPS IIID - Sanfilippo syndrome D
Reactome : MPS IV - Morquio syndrome A
Reactome : MPS IV - Morquio syndrome B
Reactome : MPS IX - Natowicz syndrome
Reactome : MPS VI - Maroteaux-Lamy syndrome
Reactome : MPS VII - Sly syndrome
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Mucopolysaccharidoses
Reactome : Myoclonic epilepsy of Lafora

Protein

UniProt : B3KUI5
UniProt : Q12794
HPRD : 06146

Clone

Cloning Information : 139846
HIP Master Clone ID : 116113
Original Clone ID : FLH139846.01X

Organism


TAX_ID : 9606
Species Specific ID: 3373

Genome

Chromosome : 3
Map Location : 3p21.3-p21.2
Ensembl : ENSG00000114378

Reagents

Labome : HYAL1-antibody