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HHIP (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HHIP
Gene Name:  hedgehog interacting protein
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH141434.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00005768

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2103nts         Open reading frame : 1 to 2103
Reference Sequence Alignment

ATGCTGAAGATGCTCTCCTTTAAGCTGCTGCTGCTGGCCGTGGCTCTGGGCTTCTTTGAA
GGAGATGCTAAGTTTGGGGAAAGAAACGAAGGGAGCGGAGCAAGGAGGAGAAGGTGCCTG
AATGGGAACCCCCCGAAGCGCCTGAAAAGGAGAGACAGGAGGATGATGTCCCAGCTGGAG
CTGCTGAGTGGGGGAGAGATGCTGTGCGGTGGCTTCTACCCTCGGCTGTCCTGCTGCCTG
CGGAGTGACAGCCCGGGGCTAGGGCGCCTGGAGAATAAGATATTTTCTGTTACCAACAAC
ACAGAATGTGGGAAGTTACTGGAGGAAATCAAATGTGCACTTTGCTCTCCACATTCTCAA
AGCCTGTTCCACTCACCTGAGAGAGAAGTCTTGGAAAGAGACCTAGTACTTCCTCTGCTC
TGCAAAGACTATTGCAAAGAATTCTTTTACACTTGCCGAGGCCATATTCCAGGTTTCCTT
CAAACAACTGCGGATGAGTTTTGCTTTTACTATGCAAGAAAAGATGGTGGGTTGTGCTTT
CCAGATTTTCCAAGAAAACAAGTCAGAGGACCAGCATCTAACTACTTGGACCAGATGGAA
GAATATGACAAAGTGGAAGAGATCAGCAGAAAGCACAAACACAACTGCTTCTGTATTCAG
GAGGTTGTGAGTGGGCTGCGGCAGCCCGTTGGTGCCCTGCATAGTGGGGATGGCTCGCAA
CGTCTCTTCATTCTGGAAAAAGAAGGTTATGTGAAGATACTTACCCCTGAAGGAGAAATT
TTCAAGGAGCCTTATTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAT
GAAAGAGGACTGCTAAGCCTCGCATTCCATCCCAATTACAAGAAAAATGGAAAGTTGTAT
GTGTCCTATACCACCAACCAAGAACGGTGGGCTATCGGGCCTCATGACCACATTCTTAGG
GTTGTGGAATACACAGTATCCAGAAAAAATCCACACCAAGTTGATTTGAGAACAGCCAGA
GTCTTTCTTGAAGTTGCAGAACTCCACAGAAAGCATCTGGGAGGACAACTGCTCTTTGGC
CCTGACGGCTTTTTGTACATCATTCTTGGTGATGGGATGATTACACTGGATGATATGGAA
GAAATGGATGGGTTAAGTGATTTCACAGGCTCAGTGCTACGGCTGGATGTGGACACAGAC
ATGTGCAACGTGCCTTATTCCATACCAAGGAGCAACCCACACTTCAACAGCACCAACCAG
CCCCCCGAAGTGTTTGCTCATGGGCTCCACGATCCAGGCAGATGTGCTGTGGATAGACAT
CCCACTGATATAAACATCAATTTAACGATACTGTGTTCAGACTCCAATGGAAAAAACAGA
TCATCAGCCAGAATTCTACAGATAATAAAGGGGAAAGATTATGAAAGTGAGCCATCACTT
TTAGAATTCAAGCCATTCAGTAATGGTCCTTTGGTTGGTGGATTTGTATACCGGGGCTGC
CAGTCAGAAAGATTGTATGGAAGCTACGTGTTTGGAGATCGTAATGGGAATTTCCTAACT
CTCCAGCAAAGTCCTGTGACAAAGCAGTGGCAAGAAAAACCACTCTGTCTCGGCACTAGT
GGGTCCTGTAGAGGCTACTTTTCCGGTCACATCTTGGGATTTGGAGAAGATGAACTAGGT
GAAGTTTACATTTTATCAAGCAGTAAAAGTATGACCCAGACTCACAATGGAAAACTCTAC
AAAATTGTAGATCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGCCACGGTACAACCT
GCACAGACACTGACTTCAGAGTGCTCCAGGCTCTGTCGAAACGGCTACTGCACCCCCACG
GGAAAGTGCTGCTGCAGTCCAGGCTGGGAGGGGGACTTCTGCAGAACTGCAAAATGTGAG
CCAGCATGTCGTCATGGAGGTGTCTGTGTTAGACCGAACAAGTGCCTCTGTAAAAAAGGA
TATCTTGGTCCTCAATGTGAACAAGTGGACAGAAACATCCGCAGAGTGACCAGGGCAGGT
ATTCTTGATCAGATCATTGACATGACATCTTACTTGCTGGATCTAACAAGTTACATTGTA
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2609
Start on reference sequence 507
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HHIP
Symbol Nomenclature : HHIP
SYNONYM : HIP
Designation : hedgehog-interacting protein
Full Nomenclature : hedgehog interacting protein
GENEID : 64399
Locus Tag : UNQ5825/PRO19644
GI : 61364376
GenBank Accession : AY890591
HGNC : 14866
MIM : 606178
Target GenBank: BC025311

 

Reference Sequence Alignment

  

HsCD00005768      ATGCTGAAGATGCTCTCCTTTAAGCTGCTGCTGCTGGCCGTGGCTCTGGGCTTCTTTGAA
NM_022475.2       ATGCTGAAGATGCTCTCCTTTAAGCTGCTGCTGCTGGCCGTGGCTCTGGGCTTCTTTGAA
                  ************************************************************

HsCD00005768      GGAGATGCTAAGTTTGGGGAAAGAAACGAAGGGAGCGGAGCAAGGAGGAGAAGGTGCCTG
NM_022475.2       GGAGATGCTAAGTTTGGGGAAAGAAACGAAGGGAGCGGAGCAAGGAGGAGAAGGTGCCTG
                  ************************************************************

HsCD00005768      AATGGGAACCCCCCGAAGCGCCTGAAAAGGAGAGACAGGAGGATGATGTCCCAGCTGGAG
NM_022475.2       AATGGGAACCCCCCGAAGCGCCTGAAAAGGAGAGACAGGAGGATGATGTCCCAGCTGGAG
                  ************************************************************

HsCD00005768      CTGCTGAGTGGGGGAGAGATGCTGTGCGGTGGCTTCTACCCTCGGCTGTCCTGCTGCCTG
NM_022475.2       CTGCTGAGTGGGGGAGAGATGCTGTGCGGTGGCTTCTACCCTCGGCTGTCCTGCTGCCTG
                  ************************************************************

HsCD00005768      CGGAGTGACAGCCCGGGGCTAGGGCGCCTGGAGAATAAGATATTTTCTGTTACCAACAAC
NM_022475.2       CGGAGTGACAGCCCGGGGCTAGGGCGCCTGGAGAATAAGATATTTTCTGTTACCAACAAC
                  ************************************************************

HsCD00005768      ACAGAATGTGGGAAGTTACTGGAGGAAATCAAATGTGCACTTTGCTCTCCACATTCTCAA
NM_022475.2       ACAGAATGTGGGAAGTTACTGGAGGAAATCAAATGTGCACTTTGCTCTCCACATTCTCAA
                  ************************************************************

HsCD00005768      AGCCTGTTCCACTCACCTGAGAGAGAAGTCTTGGAAAGAGACCTAGTACTTCCTCTGCTC
NM_022475.2       AGCCTGTTCCACTCACCTGAGAGAGAAGTCTTGGAAAGAGACCTAGTACTTCCTCTGCTC
                  ************************************************************

HsCD00005768      TGCAAAGACTATTGCAAAGAATTCTTTTACACTTGCCGAGGCCATATTCCAGGTTTCCTT
NM_022475.2       TGCAAAGACTATTGCAAAGAATTCTTTTACACTTGCCGAGGCCATATTCCAGGTTTCCTT
                  ************************************************************

HsCD00005768      CAAACAACTGCGGATGAGTTTTGCTTTTACTATGCAAGAAAAGATGGTGGGTTGTGCTTT
NM_022475.2       CAAACAACTGCGGATGAGTTTTGCTTTTACTATGCAAGAAAAGATGGTGGGTTGTGCTTT
                  ************************************************************

HsCD00005768      CCAGATTTTCCAAGAAAACAAGTCAGAGGACCAGCATCTAACTACTTGGACCAGATGGAA
NM_022475.2       CCAGATTTTCCAAGAAAACAAGTCAGAGGACCAGCATCTAACTACTTGGACCAGATGGAA
                  ************************************************************

HsCD00005768      GAATATGACAAAGTGGAAGAGATCAGCAGAAAGCACAAACACAACTGCTTCTGTATTCAG
NM_022475.2       GAATATGACAAAGTGGAAGAGATCAGCAGAAAGCACAAACACAACTGCTTCTGTATTCAG
                  ************************************************************

HsCD00005768      GAGGTTGTGAGTGGGCTGCGGCAGCCCGTTGGTGCCCTGCATAGTGGGGATGGCTCGCAA
NM_022475.2       GAGGTTGTGAGTGGGCTGCGGCAGCCCGTTGGTGCCCTGCATAGTGGGGATGGCTCGCAA
                  ************************************************************

HsCD00005768      CGTCTCTTCATTCTGGAAAAAGAAGGTTATGTGAAGATACTTACCCCTGAAGGAGAAATT
NM_022475.2       CGTCTCTTCATTCTGGAAAAAGAAGGTTATGTGAAGATACTTACCCCTGAAGGAGAAATT
                  ************************************************************

HsCD00005768      TTCAAGGAGCCTTATTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAT
NM_022475.2       TTCAAGGAGCCTTATTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAT
                  ************************************************************

HsCD00005768      GAAAGAGGACTGCTAAGCCTCGCATTCCATCCCAATTACAAGAAAAATGGAAAGTTGTAT
NM_022475.2       GAAAGAGGACTGCTAAGCCTCGCATTCCATCCCAATTACAAGAAAAATGGAAAGTTGTAT
                  ************************************************************

HsCD00005768      GTGTCCTATACCACCAACCAAGAACGGTGGGCTATCGGGCCTCATGACCACATTCTTAGG
NM_022475.2       GTGTCCTATACCACCAACCAAGAACGGTGGGCTATCGGGCCTCATGACCACATTCTTAGG
                  ************************************************************

HsCD00005768      GTTGTGGAATACACAGTATCCAGAAAAAATCCACACCAAGTTGATTTGAGAACAGCCAGA
NM_022475.2       GTTGTGGAATACACAGTATCCAGAAAAAATCCACACCAAGTTGATTTGAGAACAGCCAGA
                  ************************************************************

HsCD00005768      GTCTTTCTTGAAGTTGCAGAACTCCACAGAAAGCATCTGGGAGGACAACTGCTCTTTGGC
NM_022475.2       GTCTTTCTTGAAGTTGCAGAACTCCACAGAAAGCATCTGGGAGGACAACTGCTCTTTGGC
                  ************************************************************

HsCD00005768      CCTGACGGCTTTTTGTACATCATTCTTGGTGATGGGATGATTACACTGGATGATATGGAA
NM_022475.2       CCTGACGGCTTTTTGTACATCATTCTTGGTGATGGGATGATTACACTGGATGATATGGAA
                  ************************************************************

HsCD00005768      GAAATGGATGGGTTAAGTGATTTCACAGGCTCAGTGCTACGGCTGGATGTGGACACAGAC
NM_022475.2       GAAATGGATGGGTTAAGTGATTTCACAGGCTCAGTGCTACGGCTGGATGTGGACACAGAC
                  ************************************************************

HsCD00005768      ATGTGCAACGTGCCTTATTCCATACCAAGGAGCAACCCACACTTCAACAGCACCAACCAG
NM_022475.2       ATGTGCAACGTGCCTTATTCCATACCAAGGAGCAACCCACACTTCAACAGCACCAACCAG
                  ************************************************************

HsCD00005768      CCCCCCGAAGTGTTTGCTCATGGGCTCCACGATCCAGGCAGATGTGCTGTGGATAGACAT
NM_022475.2       CCCCCCGAAGTGTTTGCTCATGGGCTCCACGATCCAGGCAGATGTGCTGTGGATAGACAT
                  ************************************************************

HsCD00005768      CCCACTGATATAAACATCAATTTAACGATACTGTGTTCAGACTCCAATGGAAAAAACAGA
NM_022475.2       CCCACTGATATAAACATCAATTTAACGATACTGTGTTCAGACTCCAATGGAAAAAACAGA
                  ************************************************************

HsCD00005768      TCATCAGCCAGAATTCTACAGATAATAAAGGGGAAAGATTATGAAAGTGAGCCATCACTT
NM_022475.2       TCATCAGCCAGAATTCTACAGATAATAAAGGGGAAAGATTATGAAAGTGAGCCATCACTT
                  ************************************************************

HsCD00005768      TTAGAATTCAAGCCATTCAGTAATGGTCCTTTGGTTGGTGGATTTGTATACCGGGGCTGC
NM_022475.2       TTAGAATTCAAGCCATTCAGTAATGGTCCTTTGGTTGGTGGATTTGTATACCGGGGCTGC
                  ************************************************************

HsCD00005768      CAGTCAGAAAGATTGTATGGAAGCTACGTGTTTGGAGATCGTAATGGGAATTTCCTAACT
NM_022475.2       CAGTCAGAAAGATTGTATGGAAGCTACGTGTTTGGAGATCGTAATGGGAATTTCCTAACT
                  ************************************************************

HsCD00005768      CTCCAGCAAAGTCCTGTGACAAAGCAGTGGCAAGAAAAACCACTCTGTCTCGGCACTAGT
NM_022475.2       CTCCAGCAAAGTCCTGTGACAAAGCAGTGGCAAGAAAAACCACTCTGTCTCGGCACTAGT
                  ************************************************************

HsCD00005768      GGGTCCTGTAGAGGCTACTTTTCCGGTCACATCTTGGGATTTGGAGAAGATGAACTAGGT
NM_022475.2       GGGTCCTGTAGAGGCTACTTTTCCGGTCACATCTTGGGATTTGGAGAAGATGAACTAGGT
                  ************************************************************

HsCD00005768      GAAGTTTACATTTTATCAAGCAGTAAAAGTATGACCCAGACTCACAATGGAAAACTCTAC
NM_022475.2       GAAGTTTACATTTTATCAAGCAGTAAAAGTATGACCCAGACTCACAATGGAAAACTCTAC
                  ************************************************************

HsCD00005768      AAAATTGTAGATCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGCCACGGTACAACCT
NM_022475.2       AAAATTGTAGATCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGCCACGGTACAACCT
                  ************************************************************

HsCD00005768      GCACAGACACTGACTTCAGAGTGCTCCAGGCTCTGTCGAAACGGCTACTGCACCCCCACG
NM_022475.2       GCACAGACACTGACTTCAGAGTGCTCCAGGCTCTGTCGAAACGGCTACTGCACCCCCACG
                  ************************************************************

HsCD00005768      GGAAAGTGCTGCTGCAGTCCAGGCTGGGAGGGGGACTTCTGCAGAACTGCAAAATGTGAG
NM_022475.2       GGAAAGTGCTGCTGCAGTCCAGGCTGGGAGGGGGACTTCTGCAGAACTGCAAAATGTGAG
                  ************************************************************

HsCD00005768      CCAGCATGTCGTCATGGAGGTGTCTGTGTTAGACCGAACAAGTGCCTCTGTAAAAAAGGA
NM_022475.2       CCAGCATGTCGTCATGGAGGTGTCTGTGTTAGACCGAACAAGTGCCTCTGTAAAAAAGGA
                  ************************************************************

HsCD00005768      TATCTTGGTCCTCAATGTGAACAAGTGGACAGAAACATCCGCAGAGTGACCAGGGCAGGT
NM_022475.2       TATCTTGGTCCTCAATGTGAACAAGTGGACAGAAACATCCGCAGAGTGACCAGGGCAGGT
                  ************************************************************

HsCD00005768      ATTCTTGATCAGATCATTGACATGACATCTTACTTGCTGGATCTAACAAGTTACATTGTA
NM_022475.2       ATTCTTGATCAGATCATTGACATGACATCTTACTTGCTGGATCTAACAAGTTACATTGTA
                  ************************************************************

HsCD00005768      TAG
NM_022475.2       TAG
                  ***

Pathways

NCI : Signaling events mediated by the Hedgehog family

Protein

SMART domain : EGF : Epidermal growth factor-like domain.
UniProt : Q96QV1
HPRD : 06937

Clone

Cloning Information : 141434
HIP Master Clone ID : 117701
Original Clone ID : FLH141434.01X

Organism


TAX_ID : 9606
Species Specific ID: 64399

Genome

Chromosome : 4
Map Location : 4q28-q32