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SLC10A6 (H. sapiens ) in pPICZa

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Explanation of Terms


Gene: Gene Symbol:  SLC10A6
Gene Name:  solute carrier family 10 (sodium/bile acid cotransporter), member 6
Sequence             
Original Clone ID: TPDB-0101
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pPICZa               Format:  FUSION
Source: Center for the X-ray Structure Determination of Human Transporters
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI:Biology
Center for the X-ray Structure Determination of Human Transporters
HsCD00583098

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1128nts         Open reading frame : 21 to 1148
Reference Sequence Alignment

ACGATGACGATAAGTCTAGAAGAGCCAATTGTTCCAGCAGCTCAGCCTGCCCTGCCAACA
GTTCAGAGGAGGAGCTGCCAGTGGGACTGGAGGTGCATGGAAACCTGGAGCTCGTTTTCA
CAGTGGTGTCCACTGTGATGATGGGGCTGCTCATGTTCTCTTTGGGATGTTCCGTGGAGA
TCCGGAAGCTGTGGTCGCACATCAGGAGACCCTGGGGCATTGCTGTGGGACTGCTCTGCC
AGTTTGGGCTCATGCCTTTTACAGCTTATCTCCTGGCCATTAGCTTTTCTCTGAAGCCAG
TCCAAGCTATTGCTGTTCTCATCATGGGCTGCTGCCCGGGGGGCACCATCTCTAACGTTT
TCACCTTCTGGGTTGATGGAGATATGGATCTCAGCATCAGTATGACAACCTGTTCCACCG
TGGCCGCCCTGGGAATGATGCCACTCTGCATTTATCTCTACACCTGGTCCTGGAGTCTTC
AGCAGAATCTCACCATTCCTTATCAGAACATAGGAATTACCCTTGTGTGCCTGACCATTC
CTGTGGCCTTTGGTGTCTATGTGAATTACAGATGGCCAAAACAATCCAAAATCATTCTCA
AGATTGGGGCCGTTGTTGGTGGGGTCCTCCTTCTGGTGGTCGCAGTTGCTGGTGTGGTCC
TGGCGAAAGGATCTTGGAATTCAGACATCACCCTTCTGACCATCAGTTTCATCTTTCCTT
TGATTGGCCATGTCACGGGTTTTCTGCTGGCACTTTTTACCCACCAGTCTTGGCAAAGGT
GCAGGACAATTTCCTTAGAAACTGGAGCTCAGAATATTCAGATGTGCATCACCATGCTCC
AGTTATCTTTCACTGCTGAGCACTTGGTCCAGATGTTGAGTTTCCCACTGGCCTATGGAC
TCTTCCAGCTGATAGATGGATTTCTTATTGTTGCAGCATATCAGACGTACAAGAGGAGAT
TGAAGAACAAACATGGAAAAAAGAACTCAGGTTGCACAGAAGTCTGCCATACGAGGAAAT
CGACTTCTTCCAGAGAGACCAATGCCTTCTTGGAGGTGAATGAAGAAGGTGCCATCACTC
CTGGGCCACCAGGGCCAATGGATTGCCACAGGGCTCTCGAGCCAGTTGGCCACATCACTT
CATGTGAAGGTACCAGAGGTTCTCACCACCATCACCACCATCACCACCATCACTAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
aaaaaccatggactacaaggacgatgacgataagtctaga

3' Linker Sequence:
ggtaccagaggttctcaccaccatcaccaccatcaccaccatcactaataa

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  Pichia    Marker:  zeocin
Host Type:  bacterial    Marker:  zeocin
Bacterial Selection Condition:  50 ug/ml zeocin
Growth Condition:  Growth in low salt LB with 50ug/ml zeocin at 37 degrees C
Comments:  None
Protein Expression Results: ProteinExpressed: Protin_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Not_Applicable
Find experimental protocols in TransportPDB-0101
Recommended expression in: Pichia Pastoris, KM71H
       DyNA Vector Map

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Vector Name: pPICZa

pPICZa in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  AOX1
Reverse:  pAOX1 Reverse
Description: with tag/fusion/marker assay, multiple cloning site clone, expression in Pichia pastoris, yeast expression expression with AOX1 and MCS and c-myc and His and TEF1 and EM7 and CYC1 and pUC ori and ZeoR; zeocin resistance in bacterial, zeocin resistance in Pichia;
Comments: None
Size (bp): 3329
Parent Vector: None
Empty Vector: None
Properties: expression in Pichia pastoris, multiple cloning site, with tag/fusion/marker, yeast expression
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 932 1011
bacterial origin pUC ori pUC origin 2602 3275
promoter EM7 EM7 promoter 1831 1898
promoter AOX1 AOX1 promoter 1 941
promoter TEF1 fragment containing TEF1 promoter 1419 1830
selectable marker ZeoR Zeocin resistance 1899 2273
tag c-myc N-terminal C-myc tag 1012 1044
tag His C-terminal 6xHis tag 1057 1077
trxn termination sequence CYC1 CYC1 transcription termination region 2274 2591

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SLC10A6
Symbol Nomenclature : SLC10A6
SYNONYM : SOAT
Designation : sodium-dependent organic anion transporter|solute carrier family 10 (sodium/bile acid cotransporter family), member 6|solute carrier family 10 member 6
Full Nomenclature : solute carrier family 10 (sodium/bile acid cotransporter), member 6
GENEID : 345274
GI : 37537552
GenBank Accession : NM_197965
HGNC : 30603
MIM : 613366
Vega : OTTHUMG00000130596
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00583098      ACGATGACGATAAGTCTAGAAGAGCCAATTGTTCCAGCAGCTCAGCCTGCCCTGCCAACA
NM_197965.2       -----------------ATGAGAGCCAATTGTTCCAGCAGCTCAGCCTGCCCTGCCAACA
                                   * .****************************************

HsCD00583098      GTTCAGAGGAGGAGCTGCCAGTGGGACTGGAGGTGCATGGAAACCTGGAGCTCGTTTTCA
NM_197965.2       GTTCAGAGGAGGAGCTGCCAGTGGGACTGGAGGTGCATGGAAACCTGGAGCTCGTTTTCA
                  ************************************************************

HsCD00583098      CAGTGGTGTCCACTGTGATGATGGGGCTGCTCATGTTCTCTTTGGGATGTTCCGTGGAGA
NM_197965.2       CAGTGGTGTCCACTGTGATGATGGGGCTGCTCATGTTCTCTTTGGGATGTTCCGTGGAGA
                  ************************************************************

HsCD00583098      TCCGGAAGCTGTGGTCGCACATCAGGAGACCCTGGGGCATTGCTGTGGGACTGCTCTGCC
NM_197965.2       TCCGGAAGCTGTGGTCGCACATCAGGAGACCCTGGGGCATTGCTGTGGGACTGCTCTGCC
                  ************************************************************

HsCD00583098      AGTTTGGGCTCATGCCTTTTACAGCTTATCTCCTGGCCATTAGCTTTTCTCTGAAGCCAG
NM_197965.2       AGTTTGGGCTCATGCCTTTTACAGCTTATCTCCTGGCCATTAGCTTTTCTCTGAAGCCAG
                  ************************************************************

HsCD00583098      TCCAAGCTATTGCTGTTCTCATCATGGGCTGCTGCCCGGGGGGCACCATCTCTAACGTTT
NM_197965.2       TCCAAGCTATTGCTGTTCTCATCATGGGCTGCTGCCCGGGGGGCACCATCTCTAACATTT
                  ********************************************************.***

HsCD00583098      TCACCTTCTGGGTTGATGGAGATATGGATCTCAGCATCAGTATGACAACCTGTTCCACCG
NM_197965.2       TCACCTTCTGGGTTGATGGAGATATGGATCTCAGCATCAGTATGACAACCTGTTCCACCG
                  ************************************************************

HsCD00583098      TGGCCGCCCTGGGAATGATGCCACTCTGCATTTATCTCTACACCTGGTCCTGGAGTCTTC
NM_197965.2       TGGCCGCCCTGGGAATGATGCCACTCTGCATTTATCTCTACACCTGGTCCTGGAGTCTTC
                  ************************************************************

HsCD00583098      AGCAGAATCTCACCATTCCTTATCAGAACATAGGAATTACCCTTGTGTGCCTGACCATTC
NM_197965.2       AGCAGAATCTCACCATTCCTTATCAGAACATAGGAATTACCCTTGTGTGCCTGACCATTC
                  ************************************************************

HsCD00583098      CTGTGGCCTTTGGTGTCTATGTGAATTACAGATGGCCAAAACAATCCAAAATCATTCTCA
NM_197965.2       CTGTGGCCTTTGGTGTCTATGTGAATTACAGATGGCCAAAACAATCCAAAATCATTCTCA
                  ************************************************************

HsCD00583098      AGATTGGGGCCGTTGTTGGTGGGGTCCTCCTTCTGGTGGTCGCAGTTGCTGGTGTGGTCC
NM_197965.2       AGATTGGGGCCGTTGTTGGTGGGGTCCTCCTTCTGGTGGTCGCAGTTGCTGGTGTGGTCC
                  ************************************************************

HsCD00583098      TGGCGAAAGGATCTTGGAATTCAGACATCACCCTTCTGACCATCAGTTTCATCTTTCCTT
NM_197965.2       TGGCGAAAGGATCTTGGAATTCAGACATCACCCTTCTGACCATCAGTTTCATCTTTCCTT
                  ************************************************************

HsCD00583098      TGATTGGCCATGTCACGGGTTTTCTGCTGGCACTTTTTACCCACCAGTCTTGGCAAAGGT
NM_197965.2       TGATTGGCCATGTCACGGGTTTTCTGCTGGCACTTTTTACCCACCAGTCTTGGCAAAGGT
                  ************************************************************

HsCD00583098      GCAGGACAATTTCCTTAGAAACTGGAGCTCAGAATATTCAGATGTGCATCACCATGCTCC
NM_197965.2       GCAGGACAATTTCCTTAGAAACTGGAGCTCAGAATATTCAGATGTGCATCACCATGCTCC
                  ************************************************************

HsCD00583098      AGTTATCTTTCACTGCTGAGCACTTGGTCCAGATGTTGAGTTTCCCACTGGCCTATGGAC
NM_197965.2       AGTTATCTTTCACTGCTGAGCACTTGGTCCAGATGTTGAGTTTCCCACTGGCCTATGGAC
                  ************************************************************

HsCD00583098      TCTTCCAGCTGATAGATGGATTTCTTATTGTTGCAGCATATCAGACGTACAAGAGGAGAT
NM_197965.2       TCTTCCAGCTGATAGATGGATTTCTTATTGTTGCAGCATATCAGACGTACAAGAGGAGAT
                  ************************************************************

HsCD00583098      TGAAGAACAAACATGGAAAAAAGAACTCAGGTTGCACAGAAGTCTGCCATACGAGGAAAT
NM_197965.2       TGAAGAACAAACATGGAAAAAAGAACTCAGGTTGCACAGAAGTCTGCCATACGAGGAAAT
                  ************************************************************

HsCD00583098      CGACTTCTTCCAGAGAGACCAATGCCTTCTTGGAGGTGAATGAAGAAGGTGCCATCACTC
NM_197965.2       CGACTTCTTCCAGAGAGACCAATGCCTTCTTGGAGGTGAATGAAGAAGGTGCCATCACTC
                  ************************************************************

HsCD00583098      CTGGGCCACCAGGGCCAATGGATTGCCACAGGGCTCTCGAGCCAGTTGGCCACATCACTT
NM_197965.2       CTGGGCCACCAGGGCCAATGGATTGCCACAGGGCTCTCGAGCCAGTTGGCCACATCACTT
                  ************************************************************

HsCD00583098      CATGTGAAGGTACCAGAGGTTCTCACCACCATCACCACCATCACCACCATCACTAATAA
NM_197965.2       CATGTGAATAG------------------------------------------------
                  ******** .                                                 

Pathways

Reactome : Bile acid and bile salt metabolism
Reactome : Bile salt and organic anion SLC transporters
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Recycling of bile acids and salts
Reactome : SLC-mediated transmembrane transport
Reactome : Transmembrane transport of small molecules
Reactome : Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds

Protein

Structural Annotation Wiki : TransportPDB-0101
HPRD : 11593

Clone

Cloning Information : 634452
Original Clone ID : TPDB-0101

Experimental

TargetTrack - Experimental data : TransportPDB-0101

Organism


TAX_ID : 9606
Species Specific ID: 345274

Genome

Chromosome : 4
Map Location : 4q21.3
Ensembl : ENSG00000145283

Reagents

Labome : SLC10A6-antibody