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MDC1 (Homo sapiens) in pET15_NESG (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MDC1
Gene Name:  mediator of DNA-damage checkpoint 1
Sequence             
Original Clone ID: HR9209A-29-139-15.5
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pET15_NESG               Format:  CLOSED
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Northeast Structural Genomics Consortium
PSI:Biology
HsCD00584459

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 339nts         Open reading frame : 51 to 389

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGAGCCAG
TAGGGCGGCTACATATCTTTAGTGGTGCCCATGGACCAGAAAAAGATTTCCCACTACACC
TCGGGAAGAATGTGGTAGGCCGAATGCCTGACTGCTCTGTGGCCCTGCCCTTTCCATCTA
TCTCCAAACAACATGCAGAGATTGAAATCTTAGCCTGGGACAAGGCACCTATCCTCCGAG
ACTGTGGGAGCCTTAATGGTACTCAAATCCTGAGACCTCCTAAGGTTTTGAGCCCTGGGG
TGAGTCACCGTCTGAGGGACCAGGAATTGATTCTCTTTGCTGACTTGCTCTGCCAGTACC
ATCGCCTGGATGTCTCTCTGCCCTTTTAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAA
GGAAGCTGAGTTGGCTGCTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT

3' Linker Sequence:
CTCGAGATCCGGCTGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
Find experimental protocols in HR9209A
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MDC1
Symbol Nomenclature : MDC1
SYNONYM : NFBD1
Designation : homologue to Drosophila photoreceptor protein calphotin|mediator of DNA damage checkpoint 1|mediator of DNA damage checkpoint protein 1|nuclear factor with BRCT domains 1
Full Nomenclature : mediator of DNA-damage checkpoint 1
GENEID : 9656
Locus Tag : DAAP-285E11.7
GI : 132626688
GenBank Accession : NM_014641
HGNC : 21163
MIM : 607593
Vega : OTTHUMG00000031075
Target GenBank: None

 

Pathways

NCI : ATM pathway
Reactome : ATM mediated phosphorylation of repair proteins
Reactome : ATM mediated response to DNA double-strand break
Reactome : DNA Repair
Reactome : Double-Strand Break Repair
Reactome : Homologous Recombination Repair
Reactome : Homologous recombination repair of replication-independent double-strand breaks
Reactome : Recruitment of repair and signaling proteins to double-strand breaks

Protein

SMART domain : FHA : Forkhead associated domain
Structural Annotation Wiki : HR9209A
UniProt : Q14676
HPRD : 16252

Clone

Cloning Information : 640439
Original Clone ID : HR9209A-29-139-15.5

Experimental

TargetTrack - Experimental data : HR9209A

Organism


TAX_ID : 9606
Species Specific ID: 9656

Genome

Chromosome : 6
Map Location : 6p21.3
Ensembl : ENSG00000137337

Reagents

Labome : MDC1-antibody