PLEC (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PLEC
Gene Name:  plectin
Original Clone ID:
HR6354C-3713-4029-15.13
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00586103
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 957nts
Open reading frame: 42 to 998

GGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGGCGGCGGCAGCCACG
GCGGCTCCACCATGTCCCTGTGGGAGGTGATGCAGTCGGACCTGATCCCCGAGGAGCAGC
GGGCCCAGCTGATGACTGACTTCCAGGCCGGCCGGGTGACCAAGGAACGCATGATCATCA
TCATCATCGAGATCATTGAGAAGACAGAGATCATCCGCCAGCAGGGTCTGGCCTCCTACG
ACTACGTGCGCCGCCGCCTCACGGCTGAGGACCTGTTCGAGGCTCGGATCATCTCTCTCG
AGACCTACAACCTGCTCCGGGAGGGCACCAGGAGCCTCCGTGAGGCTCTCGAGGCGGAGT
CCGCCTGGTGCTACCTCTATGGCACGGGCTCCGTGGCTGGTGTCTACCTGCCCGGTTCCA
GGCAGACACTGAGCATCTACCAGGCCCTCAAGAAAGGGCTGCTGAGTGCCGAGGTGGCCC
GCCTGCTGCTGGAGGCACAGGCAGCCACAGGCTTCCTGCTGGACCCGGTGAAGGGGGAGC
GGCTGACTGTGGATGAGGCTGTGCGGAAGGGCCTCGTGGGGCCCGAGCTGCACGACCGCC
TGCTCTCGGCTGAGCGGGCGGTCACCGGCTACCGTGACCCCTACACCGAGCAGACCATCT
CGCTTTTCCAGGCCATGAAGAAGGAGCTGATCCCTACTGAGGAGGCCCTGCGGCTGCTGG
ATGCCCAGCTGGCCACCGGCGGCATCGTGGACCCCCGCCTGGGCTTCCACCTTCCCCTGG
AGGTGGCTTACCAGCGTGGCTACCTCAACAAGGACACGCACGACCAGCTGTCAGAGCCCA
GCGAGGTGCGCAGCTACGTGGACCCGTCCACCGACGAGCGCCTCAGCTACACGCAGCTGC
TCAGGCGGTGCCGTCGTGACGACGGCACCGGCCAGCTGCTCCTGCCACTGTCGGACGCCC
GCAAGCTGACCTTCCGTGGCCTGCGGAAGCAGATCTAGCTCGAGATCCGGCTGCTAACAA
AGCCCGAAAG

Protein sequence predicted based on nucleotide sequence

MGGGSHGGSTMSLWEVMQSDLIPEEQRAQLMTDFQAGRVTKERMIIIIIEIIEKTEIIRQ
QGLASYDYVRRRLTAEDLFEARIISLETYNLLREGTRSLREALEAESAWCYLYGTGSVAG
VYLPGSRQTLSIYQALKKGLLSAEVARLLLEAQAATGFLLDPVKGERLTVDEAVRKGLVG
PELHDRLLSAERAVTGYRDPYTEQTISLFQAMKKELIPTEEALRLLDAQLATGGIVDPRL
GFHLPLEVAYQRGYLNKDTHDQLSEPSEVRSYVDPSTDERLSYTQLLRRCRRDDGTGQLL
LPLSDARKLTFRGLRKQI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g94a,A32T;t405c,A135A;

c624t,L208L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in HR6354C
Recommended expression in:
Not Applicable