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ynhG (Salmonella typhimurium LT2) in pMCSG7 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ynhG
Gene Name:  hypothetical protein
Sequence             
Original Clone ID: APC101509.102
Keyword: None
Species: Salmonella typhimurium LT2
Type: cDNA
Vector Name: pMCSG7               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: Argonne National Laboratory
Robert Jedrzejczak
PSI:Biology
Midwest Center for Structural Genomics
StCD00586545

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1002nts         Open reading frame : 51 to 1052

CTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAAACGTG
CGTCTTTCATTACGCTAACTATTATCGGCGCGTATAGCGCGTTACAGGCAGCCTGGGCGG
TTGATTATCCATTACCGCCCGAAGGCAGCCGTCTTATTGGTCAGAATCAAACCTATACCG
TACAGGAAGGTGATAAAAACCTGCAGGCTATAGCCCGGCGTTTTGATACGGCGGCGATGC
TCATTCTTGAAGCGAACAATACGATTGCGCCGGTGCCTAAGCCCGGTACGCTAATTACCA
TTCCCTCGCAGATGCTATTGCCGGATGCGCCCAGGGAAGGCGTTATCGTCAATCTCGCGG
AGCTACGGCTGTATTATTACCCGCCGGGAGAAAACCGCGTACAGGTGTATCCCATCGGCA
TCGGTTTGCAGGGACTGGAAACTCCCGTCATGGACACCCGGATAGGGCAAAAGATCCCCA
ACCCGACGTGGACGCCGACGGCAGGCATACGCCAACGTTCGCTTGAGCGGGGGATCACGC
TGCCGCCGGTGATCCCTGCCGGGCCAAATAACCCGCTGGGATGCTATGCGCTGCGTCTGG
CGCACGGTAATGGGGAATATCTCATTCATGGCACCAGCGCGCCGGATAGCGTAGGTCTGC
GTGTGAGTTCCGGTTGCATTCGCATGAACGCGCCGGACATCAAGGCGTTATTTGCGCAGG
TCAGAACGGGGACGCCGGTAAAAGTGATTAACCAGCCAGTGAAATTCTCTGTCGAGCCGA
ATGGCATTCGTTATGTAGAGGTACACAGGCCGCTATCGCCGGAAGAAGAGCAAAACGTGC
AGACAATGCCCTATGTGTTGCCTGCGGAATTTACCGCGTTCAGAAACGCGCAAGGGGTGG
ATAGTCGCTTGGTCGATAAGGCGCTATACCGGCGAGCCGGGTATCCTGTCAGCGTGAGCG
CCGGGCAGACGCCGGCGGTAAATACGACCGCAGTCGAATCCGCTCAGAACGGTTTTGTCG
GGGAAGAGGGGCAAACGCGCGCGACGCAGTAACATTGGAAGTGGATAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
c532t,R178C
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC101509
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pMCSG7

pMCSG7 in advanced viewed

Synonyms: pMCSG-7
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expresson vector with T7 promoter, adds N-terminal His tag and TEV protease site; amp resistance; ligation independent cloning (LIC).
Comments: pET21 derivative; for routine HTP purification
Size (bp): 5286
Parent Vector: None
Empty Vector: EvNO00084271
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 12071693
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 204 235
bacterial origin ori ColE1 pBR322-type bacterial origin of replication 3280 3280
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 335 357
repressor protein gene LacI LacI coding region 890 1849
selectable marker AmpR ampicillin resistance gene 4038 4898
tag His 2 N-terminal 6xHis tag 268 285
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : ynhG
GENEID : 1252893
Locus Tag : STM1375
GI : 16419895
Target GenBank: None

 

Protein

SMART domain : LysM : Lysin motif
Structural Annotation Wiki : MCSG-APC101509

Clone

Cloning Information : 601872
Original Clone ID : APC101509.102

Experimental

TargetTrack - Experimental data : MCSG-APC101509

Organism


TAX_ID : 99287
Species Specific ID: 1252893