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ycfS (Escherichia coli str. K-12 substr. DH10B) in pMCSG73 (Bacterial Expression)

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Explanation of Terms


Gene: Gene Symbol:  ycfS
Gene Name:  hypothetical protein
Sequence             
Original Clone ID: APC110541.102
Keyword: None
Species: Escherichia coli str. K-12 substr. DH10B
Type: cDNA
Vector Name: pMCSG73               Format:  FUSION
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
EcCD00586758

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 876nts        
Open reading frame : 7 to 882

AATGCCAACACCTGGCCGCTGCCGCCAGCGGGCAGTCGTCTGGTTGGCGAAAACAAATTT
CATGTGGTGGAAAATGACGGTGGTTCTCTGGAAGCCATCGCCAAAAAATACAACGTCGGC
TTTCTCGCTCTGTTACAGGCTAACCCCGGCGTTGATCCTTACGTACCGCGCGCGGGCAGC
GTGTTAACGATCCCGTTGCAAACCCTACTTCCAGATGCGCCGCGCGAAGGCATTGTGATC
AACATTGCGGAGCTGCGTCTCTATTACTACCCGCCGGGTAAAAATTCGGTAACCGTGTAT
CCAATAGGTATTGGTCAGTTAGGTGGTGACACGCTGACACCGACAATGGTGACCACCGTT
TCAGACAAACGTGCAAACCCAACCTGGACGCCAACGGCAAACATCCGCGCCCGTTATAAA
GCACAGGGAATTGAGTTGCCTGCGGTAGTGCCGGCTGGACCGGATAACCCAATGGGCCAT
CATGCGATTCGTCTGGCGGCCTATGGCGGCGTTTATTTGCTTCATGGTACGAACGCCGAT
TTCGGCATTGGCATGCGGGTAAGTTCTGGCTGTATTCGTCTGCGGGATGACGATATCAAA
ACACTCTTTAGCCAGGTCACCCCAGGCACCAAAGTGAATATCATCAACACTCCGATAAAA
GTCTCTGCCGAACCAAACGGTGCGCGTCTGGTTGAAGTACATCAGCCGCTGTCAGAGAAG
ATTGATGACGATCCGCAGCTGCTGCCAATTACGCTGAATAGCGCAATGCAATCATTTAAA
GATGCAGCACAAACTGACGCTGAAGTGATGCAACATGTGATGGATGTCCGTTCCGGGATG
CCGGTGGATGTCCGCCGTCATCAAGTGAGCCCACAAACGCTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC110541
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pMCSG73

pMCSG73 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  MCSG73F
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and TVMV-cleavable N-terminal NusA tag and TEV-cleavable N-terminal 6xHis and StrepII tag; Contains rate argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments: None
Size (bp): 6227
Parent Vector: Name: pMCSG53
Description:
Empty Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 4220 4808
ligation independent cloning LIC ligation independent cloning site 138 16
promoter T7 T7 promoter 1779 1795
protease cleavage site TVMV TVMV protease cleavage site 218 238
protease cleavage site TEV TEV protease cleavage site 155 175
repressor binding site lac operator lac operator 1754 1776
repressor protein gene LacI LacI CDS 2832 3791
selectable marker AmpR ampicillin resistance gene 4979 5839
tRNA ileX ileX tRNA gene 2378 2529
tRNA argU argU tRNA 1862 2218
tag NusA N-terminal NusA 249 1708
tag His N-terminal 6xHis tag 200 217
tag strep N-terminal StrepII tag 176 199
trxn termination sequence T7 term T7 terminator 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : ycfS
GENEID : 6058759
Locus Tag : ECDH10B_1185
GI : 170080764
Target GenBank: None

 

Protein

SMART domain : LysM : Lysin motif
Structural Annotation Wiki : MCSG-APC110541

Clone

Cloning Information : 630880
Original Clone ID : APC110541.102

Experimental

TargetTrack - Experimental data : MCSG-APC110541

Organism


TAX_ID : 316385
Species Specific ID: 6058759