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lmo2366 (Listeria monocytogenes EGD-e) in pMCSG68 (Bacterial Expression)

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Explanation of Terms


Gene: Gene Symbol:  lmo2366
Gene Name:  similar to transcription regulator DeoR family
Sequence             
Original Clone ID: APC106887.102
Keyword: None
Species: Listeria monocytogenes EGD-e
Type: cDNA
Vector Name: pMCSG68               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Midwest Center for Structural Genomics
Argonne National Laboratory
PSI:Biology
Michael Endres
LmCD00589111

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 759nts         Open reading frame : 51 to 809

ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGTTATCTA
TCGAACGAAAGCGTGCCATCGTCCAATATGTAAAAAGTAGAAAAATAGCAACAGTTAGCG
AACTTGCCAAACATTTTGAAGTCCACGAAGCAACCATTCGCCGCGATTTAACCTCTTTAG
AAAAAGACAAAAAGCTAAAAAGAACCCACGGCGGAGTTATGATAGAAGAAAAAGTAGTTT
CCGAACCGAATTGGAAAAAACGAAGCGAAGTGCGCTACGAAGAAAAACAACGCATTGCCA
CATTAGCAGCGACAATGGTCAAAGACGGCGATACAATCATTCTTGACGCCGGAACAACAA
CCGGACACATCGCAACCGCATTAAAAGACCGTTCCAAACTAACCGTCATAACAAACGATA
TCAACGTAGCTTCCATTATGCGTTTCTCTCCGTCTAAAGTAATTGTGACAGGCGGCGTCA
TCTACCCAGAAACTTTCATCCTTAACGGTATGATTACAAGTGGAACCTTGCAGAGCATTC
ACGTACATAAAGCATTTGTAACCACACCAGCTCTCGATATCGACAAAGGTTTAATGCACT
ACGACGAATACCTCATCCCAGCTAAGCAACAAATGCTCCACTCAGCTGATGAAGTTATCT
TAGTAACAGATCACACCAAGTTCGGCCGCATTTCACTTTACAAATACGCAGCCCTAGACG
AAATCTCTTCCATTATCACCGGAAAAGAAATAGATCCGGTACTAAAAGAACAATTTGAAG
AAAAAGGAATGCAAATTTATACAACATAACATTGGAAGTGGATAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC106887
Recommended expression in: E.coli BL21 (DE3)
       DyNA Vector Map

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Vector Name: pMCSG68

pMCSG68 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning
Comments: BglI-XbaI fragment removed from pMCSG57 and ligated into pMCSG53 digested with the same enzymes
Size (bp): 4832
Parent Vector: None
Empty Vector: EvNO00450864
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 2825 3413
ligation independent cloning LIC ligation independent cloning site 204 235
promoter T7 T7 promoter 384 400
protease cleavage site TEV TEV protease cleavage site 223 243
repressor protein gene LacI Lac I gene 1437 2396
selectable marker AmpR ampicillin resistance gene 3584 4444
tRNA ileX ileX tRNA gene 983 1134
tRNA argU argU tRNA gene 467 853
tag His N-terminal 6xHis tag 292 309
tag strep N-terminal strepII tag 250 273
trxn regulatory element lac operator lac operator sequence 359 381
trxn termination sequence T7 term T7 terminator 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : lmo2366
Designation : hypothetical protein
GENEID : 984418
Locus Tag : lmo2366
GI : 16804404
Target GenBank: None

 

Clone

Cloning Information : 630938
Original Clone ID : APC106887.102

Experimental

TargetTrack - Experimental data : MCSG-APC106887

Organism


TAX_ID : 169963
Species Specific ID: 984418