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GPSM2 (Homo sapiens) in pET15_NESG (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GPSM2
Gene Name:  G-protein signaling modulator 2
Sequence             
Original Clone ID: HR5762-1-557-14.3
Keyword: targeted domain
Species: Homo sapiens
Type: cDNA
Vector Name: pET15_NESG               Format:  FUSION
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: Northeast Structural Genomics Consortium
Rutgers University
PSI:Biology
HsCD00589607

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 1680nts         Open reading frame : 15 to 1694
Reference Sequence Alignment

ACCATCACAGCCATATGAGAGAAGCCCATTCTTTTCATGTTCGTTACAGAATGGAAGCTT
CTTGCCTAGAGCTGGCCTTGGAAGGGGAACGTCTATGTAAATCAGGAGACTGCCGCGCTG
GCGTGTCATTCTTTGAAGCTGCAGTTCAAGTTGGAACTGAAGACCTAAAAACACTTAGCG
CTATTTACAGCCAGTTGGGCAATGCTTATTTCTATTTGCATGATTATGCCAAAGCATTAG
AATATCACCATCATGATTTAACCCTTGCAAGGACTATTGGAGACCAGCTGGGGGAAGCGA
AAGCTAGTGGTAATCTGGGAAACACCTTAAAAGTTCTTGGGAATTTTGACGAAGCCATAG
TTTGTTGTCAGCGACACCTAGATATTTCCAGAGAGCTTAATGACAAGGTGGGAGAAGCAA
GAGCACTTTACAATCTTGGGAATGTGTATCATGCCAAAGGGAAAAGTTTTGGTTGTCCTG
GTCCCCAGGATGTAGGAGAATTTCCAGAAGAAGTGAGAGATGCTCTGCAGGCAGCCGTGG
ATTTTTATGAGGAAAACCTATCATTAGTGACTGCTTTGGGTGACCGAGCGGCACAAGGAC
GTGCCTTTGGAAATCTTGGAAACACACATTACCTCCTTGGCAACTTCAGGGATGCAGTTA
TAGCTCATGAGCAGCGTCTCCTTATTGCAAAAGAATTTGGAGATAAAGCAGCTGAAAGAA
GAGCATATAGCAACCTTGGAAATGCATATATATTTCTTGGTGAATTTGAAACTGCCTCGG
AATACTACAAGAAGACACTACTGTTGGCCCGACAGCTTAAAGACCGAGCTGTAGAAGCAC
AGTCTTGTTACAGTCTTGGAAATACATATACTTTACTTCAAGACTATGAAAAGGCCATTG
ATTATCATCTGAAGCACTTAGCAATTGCTCAAGAGCTGAATGATAGAATTGGTGAAGGAA
GAGCATGTTGGAGCTTAGGAAATGCATACACAGCACTAGGAAATCATGATCAAGCAATGC
ATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGATAAAAGTGGTGAACTAA
CAGCACGACTTAATCTCTCAGACCTTCAAATGGTTCTTGGTCTGAGCTACAGCACAAATA
ACTCCATAATGTCTGAAAATACTGAAATTGATAGCAGTTTGAATGGTGTACGCCCCAAGT
TGGGACGCCGGCATAGTATGGAAAATATGGAACTTATGAAGTTAACACCAGAAAAGGTAC
AGAACTGGAACAGTGAAATTCTTGCTAAGCAAAAACCTCTTATTGCCAAACCTTCTGCAA
AGCTACTCTTTGTCAACAGACTGAAGGGGAAAAAATACAAAACGAATTCCTCCACTAAAG
TTCTCCAAGATGCCAGTAATTCTATTGACCACCGAATTCCAAATTCTCAGAGGAAAATCA
GTGCAGATACTATTGGAGATGAAGGGTTCTTTGACTTATTAAGCCGATTTCAAAGCAATA
GGATGGATGATCAGAGATGTTGCTTACAAGAAAAGAACTGCCATACAGCTTCAACAACAA
CTTCTTCCACTCCCCCTAAAATGATGCTAAAAACATCATCTGTTCCTGTGGTATCCCCCA
ACACGGATGAGTTTTTAGATCTTCTTGCCAGCTCACAGAGTCGCCGTCTGGATGACCAGA
GGGCTTAGCTCGAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
a11c,D4A;c462t,C154C
                                

5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT

3' Linker Sequence:
CTCGAGATCCGGCTGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
Find experimental protocols in HR5762
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GPSM2
Symbol Nomenclature : GPSM2
SYNONYM : CMCS; DFNB82; LGN; PINS
Designation : G-protein signalling modulator 2 (AGS3-like, C. elegans)|G-protein-signaling modulator 2|mosaic protein LGN
Full Nomenclature : G-protein signaling modulator 2
GENEID : 29899
Locus Tag : RP11-475E11.4
GI : 164519122
GenBank Accession : NM_013296
HGNC : 29501
MIM : 609245
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00589607        -------ACCATCACAGCCATATGAGAGAAGCCCATTCTTTTCATGTTCGTTACAGAATG
XM_005270787.1      ATGGAGGAAAATTTGATAAGCATGAGAGAAGACCATTCTTTTCATGTTCGTTACAGAATG
NM_013296.4         ATGGAGGAAAATTTGATAAGCATGAGAGAAGACCATTCTTTTCATGTTCGTTACAGAATG
                           *..** : * ... **********.****************************

HsCD00589607        GAAGCTTCTTGCCTAGAGCTGGCCTTGGAAGGGGAACGTCTATGTAAATCAGGAGACTGC
XM_005270787.1      GAAGCTTCTTGCCTAGAGCTGGCCTTGGAAGGGGAACGTCTATGTAAATCAGGAGACTGC
NM_013296.4         GAAGCTTCTTGCCTAGAGCTGGCCTTGGAAGGGGAACGTCTATGTAAATCAGGAGACTGC
                    ************************************************************

HsCD00589607        CGCGCTGGCGTGTCATTCTTTGAAGCTGCAGTTCAAGTTGGAACTGAAGACCTAAAAACA
XM_005270787.1      CGCGCTGGCGTGTCATTCTTTGAAGCTGCAGTTCAAGTTGGAACTGAAGACCTAAAAACA
NM_013296.4         CGCGCTGGCGTGTCATTCTTTGAAGCTGCAGTTCAAGTTGGAACTGAAGACCTAAAAACA
                    ************************************************************

HsCD00589607        CTTAGCGCTATTTACAGCCAGTTGGGCAATGCTTATTTCTATTTGCATGATTATGCCAAA
XM_005270787.1      CTTAGCGCTATTTACAGCCAGTTGGGCAATGCTTATTTCTATTTGCATGATTATGCCAAA
NM_013296.4         CTTAGCGCTATTTACAGCCAGTTGGGCAATGCTTATTTCTATTTGCATGATTATGCCAAA
                    ************************************************************

HsCD00589607        GCATTAGAATATCACCATCATGATTTAACCCTTGCAAGGACTATTGGAGACCAGCTGGGG
XM_005270787.1      GCATTAGAATATCACCATCATGATTTAACCCTTGCAAGGACTATTGGAGACCAGCTGGGG
NM_013296.4         GCATTAGAATATCACCATCATGATTTAACCCTTGCAAGGACTATTGGAGACCAGCTGGGG
                    ************************************************************

HsCD00589607        GAAGCGAAAGCTAGTGGTAATCTGGGAAACACCTTAAAAGTTCTTGGGAATTTTGACGAA
XM_005270787.1      GAAGCGAAAGCTAGTGGTAATCTGGGAAACACCTTAAAAGTTCTTGGGAATTTTGACGAA
NM_013296.4         GAAGCGAAAGCTAGTGGTAATCTGGGAAACACCTTAAAAGTTCTTGGGAATTTTGACGAA
                    ************************************************************

HsCD00589607        GCCATAGTTTGTTGTCAGCGACACCTAGATATTTCCAGAGAGCTTAATGACAAGGTGGGA
XM_005270787.1      GCCATAGTTTGTTGTCAGCGACACCTAGATATTTCCAGAGAGCTTAATGACAAGGTGGGA
NM_013296.4         GCCATAGTTTGTTGTCAGCGACACCTAGATATTTCCAGAGAGCTTAATGACAAGGTGGGA
                    ************************************************************

HsCD00589607        GAAGCAAGAGCACTTTACAATCTTGGGAATGTGTATCATGCCAAAGGGAAAAGTTTTGGT
XM_005270787.1      GAAGCAAGAGCACTTTACAATCTTGGGAATGTGTATCATGCCAAAGGGAAAAGTTTTGGT
NM_013296.4         GAAGCAAGAGCACTTTACAATCTTGGGAATGTGTATCATGCCAAAGGGAAAAGTTTTGGT
                    ************************************************************

HsCD00589607        TGTCCTGGTCCCCAGGATGTAGGAGAATTTCCAGAAGAAGTGAGAGATGCTCTGCAGGCA
XM_005270787.1      TGCCCTGGTCCCCAGGATGTAGGAGAATTTCCAGAAGAAGTGAGAGATGCTCTGCAGGCA
NM_013296.4         TGCCCTGGTCCCCAGGATGTAGGAGAATTTCCAGAAGAAGTGAGAGATGCTCTGCAGGCA
                    ** *********************************************************

HsCD00589607        GCCGTGGATTTTTATGAGGAAAACCTATCATTAGTGACTGCTTTGGGTGACCGAGCGGCA
XM_005270787.1      GCCGTGGATTTTTATGAGGAAAACCTATCATTAGTGACTGCTTTGGGTGACCGAGCGGCA
NM_013296.4         GCCGTGGATTTTTATGAGGAAAACCTATCATTAGTGACTGCTTTGGGTGACCGAGCGGCA
                    ************************************************************

HsCD00589607        CAAGGACGTGCCTTTGGAAATCTTGGAAACACACATTACCTCCTTGGCAACTTCAGGGAT
XM_005270787.1      CAAGGACGTGCCTTTGGAAATCTTGGAAACACACATTACCTCCTTGGCAACTTCAGGGAT
NM_013296.4         CAAGGACGTGCCTTTGGAAATCTTGGAAACACACATTACCTCCTTGGCAACTTCAGGGAT
                    ************************************************************

HsCD00589607        GCAGTTATAGCTCATGAGCAGCGTCTCCTTATTGCAAAAGAATTTGGAGATAAAGCAGCT
XM_005270787.1      GCAGTTATAGCTCATGAGCAGCGTCTCCTTATTGCAAAAGAATTTGGAGATAAAGCAGCT
NM_013296.4         GCAGTTATAGCTCATGAGCAGCGTCTCCTTATTGCAAAAGAATTTGGAGATAAAGCAGCT
                    ************************************************************

HsCD00589607        GAAAGAAGAGCATATAGCAACCTTGGAAATGCATATATATTTCTTGGTGAATTTGAAACT
XM_005270787.1      GAAAGAAGAGCATATAGCAACCTTGGAAATGCATATATATTTCTTGGTGAATTTGAAACT
NM_013296.4         GAAAGAAGAGCATATAGCAACCTTGGAAATGCATATATATTTCTTGGTGAATTTGAAACT
                    ************************************************************

HsCD00589607        GCCTCGGAATACTACAAGAAGACACTACTGTTGGCCCGACAGCTTAAAGACCGAGCTGTA
XM_005270787.1      GCCTCGGAATACTACAAGAAGACACTACTGTTGGCCCGACAGCTTAAAGACCGAGCTGTA
NM_013296.4         GCCTCGGAATACTACAAGAAGACACTACTGTTGGCCCGACAGCTTAAAGACCGAGCTGTA
                    ************************************************************

HsCD00589607        GAAGCACAGTCTTGTTACAGTCTTGGAAATACATATACTTTACTTCAAGACTATGAAAAG
XM_005270787.1      GAAGCACAGTCTTGTTACAGTCTTGGAAATACATATACTTTACTTCAAGACTATGAAAAG
NM_013296.4         GAAGCACAGTCTTGTTACAGTCTTGGAAATACATATACTTTACTTCAAGACTATGAAAAG
                    ************************************************************

HsCD00589607        GCCATTGATTATCATCTGAAGCACTTAGCAATTGCTCAAGAGCTGAATGATAGAATTGGT
XM_005270787.1      GCCATTGATTATCATCTGAAGCACTTAGCAATTGCTCAAGAGCTGAATGATAGAATTGGT
NM_013296.4         GCCATTGATTATCATCTGAAGCACTTAGCAATTGCTCAAGAGCTGAATGATAGAATTGGT
                    ************************************************************

HsCD00589607        GAAGGAAGAGCATGTTGGAGCTTAGGAAATGCATACACAGCACTAGGAAATCATGATCAA
XM_005270787.1      GAAGGAAGAGCATGTTGGAGCTTAGGAAATGCATACACAGCACTAGGAAATCATGATCAA
NM_013296.4         GAAGGAAGAGCATGTTGGAGCTTAGGAAATGCATACACAGCACTAGGAAATCATGATCAA
                    ************************************************************

HsCD00589607        GCAATGCATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGATAAAAGTGGT
XM_005270787.1      GCAATGCATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGATAAAAGTGGT
NM_013296.4         GCAATGCATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGATAAAAGTGGT
                    ************************************************************

HsCD00589607        GAACTAACAGCACGACTTAATCTCTCAGACCTTCAAATGGTTCTTGGTCTGAGCTACAGC
XM_005270787.1      GAACTAACAGCACGACTTAATCTCTCAGACCTTCAAATGGTTCTTGGTCTGAGCTACAGC
NM_013296.4         GAACTAACAGCACGACTTAATCTCTCAGACCTTCAAATGGTTCTTGGTCTGAGCTACAGC
                    ************************************************************

HsCD00589607        ACAAATAACTCCATAATGTCTGAAAATACTGAAATTGATAGCAGTTTGAATGGTGTACGC
XM_005270787.1      ACAAATAACTCCATAATGTCTGAAAATACTGAAATTGATAGCAGTTTGAATGGTGTACGC
NM_013296.4         ACAAATAACTCCATAATGTCTGAAAATACTGAAATTGATAGCAGTTTGAATGGTGTACGC
                    ************************************************************

HsCD00589607        CCCAAGTTGGGACGCCGGCATAGTATGGAAAATATGGAACTTATGAAGTTAACACCAGAA
XM_005270787.1      CCCAAGTTGGGACGCCGGCATAGTATGGAAAATATGGAACTTATGAAGTTAACACCAGAA
NM_013296.4         CCCAAGTTGGGACGCCGGCATAGTATGGAAAATATGGAACTTATGAAGTTAACACCAGAA
                    ************************************************************

HsCD00589607        AAGGTACAGAACTGGAACAGTGAAATTCTTGCTAAGCAAAAACCTCTTATTGCCAAACCT
XM_005270787.1      AAGGTACAGAACTGGAACAGTGAAATTCTTGCTAAGCAAAAACCTCTTATTGCCAAACCT
NM_013296.4         AAGGTACAGAACTGGAACAGTGAAATTCTTGCTAAGCAAAAACCTCTTATTGCCAAACCT
                    ************************************************************

HsCD00589607        TCTGCAAAGCTACTCTTTGTCAACAGACTGAAGGGGAAAAAATACAAAACGAATTCCTCC
XM_005270787.1      TCTGCAAAGCTACTCTTTGTCAACAGACTGAAGGGGAAAAAATACAAAACGAATTCCTCC
NM_013296.4         TCTGCAAAGCTACTCTTTGTCAACAGACTGAAGGGGAAAAAATACAAAACGAATTCCTCC
                    ************************************************************

HsCD00589607        ACTAAAGTTCTCCAAGATGCCAGTAATTCTATTGACCACCGAATTCCAAATTCTCAGAGG
XM_005270787.1      ACTAAAGTTCTCCAAGATGCCAGTAATTCTATTGACCACCGAATTCCAAATTCTCAGAGG
NM_013296.4         ACTAAAGTTCTCCAAGATGCCAGTAATTCTATTGACCACCGAATTCCAAATTCTCAGAGG
                    ************************************************************

HsCD00589607        AAAATCAGTGCAGATACTATTGGAGATGAAGGGTTCTTTGACTTATTAAGCCGATTTCAA
XM_005270787.1      AAAATCAGTGCAGATACTATTGGAGATGAAGGGTTCTTTGACTTATTAAGCCGATTTCAA
NM_013296.4         AAAATCAGTGCAGATACTATTGGAGATGAAGGGTTCTTTGACTTATTAAGCCGATTTCAA
                    ************************************************************

HsCD00589607        AGCAATAGGATGGATGATCAGAGATGTTGCTTACAAGAAAAGAACTGCCATACAGCTTCA
XM_005270787.1      AGCAATAGGATGGATGATCAGAGATGTTGCTTACAAGAAAAGAACTGCCATACAGCTTCA
NM_013296.4         AGCAATAGGATGGATGATCAGAGATGTTGCTTACAAGAAAAGAACTGCCATACAGCTTCA
                    ************************************************************

HsCD00589607        ACAACAACTTCTTCCACTCCCCCTAAAATGATGCTAAAAACATCATCTGTTCCTGTGGTA
XM_005270787.1      ACAACAACTTCTTCCACTCCCCCTAAAATGATGCTAAAAACATCATCTGTTCCTGTGGTA
NM_013296.4         ACAACAACTTCTTCCACTCCCCCTAAAATGATGCTAAAAACATCATCTGTTCCTGTGGTA
                    ************************************************************

HsCD00589607        TCCCCCAACACGGATGAGTTTTTAGATCTTCTTGCCAGCTCACAGAGTCGCCGTCTGGAT
XM_005270787.1      TCCCCCAACACGGATGAGTTTTTAGATCTTCTTGCCAGCTCACAGAGTCGCCGTCTGGAT
NM_013296.4         TCCCCCAACACGGATGAGTTTTTAGATCTTCTTGCCAGCTCACAGAGTCGCCGTCTGGAT
                    ************************************************************

HsCD00589607        GACCAGAGGGCTTAGCTCGAG---------------------------------------
XM_005270787.1      GACCAGAGGGCTAGTTTCAGTAATTTGCCAGGGCTTCGTCTAACACAAAACAGCCAGTCG
NM_013296.4         GACCAGAGGGCTAGTTTCAGTAATTTGCCAGGGCTTCGTCTAACACAAAACAGCCAGTCG
                    ************:.  **..                                        

HsCD00589607        ------------------------------------------------------------
XM_005270787.1      GTACTTAGCCACCTGATGACTAATGACAACAAAGAGGCTGATGAAGATTTCTTTGACATC
NM_013296.4         GTACTTAGCCACCTGATGACTAATGACAACAAAGAGGCTGATGAAGATTTCTTTGACATC
                                                                                

HsCD00589607        ------------------------------------------------------------
XM_005270787.1      CTTGTAAAATGTCAAGGATCCAGATTAGATGATCAAAGATGTGCTCCACCACCTGCTACC
NM_013296.4         CTTGTAAAATGTCAAGGATCCAGATTAGATGATCAAAGATGTGCTCCACCACCTGCTACC
                                                                                

HsCD00589607        ------------------------------------------------------------
XM_005270787.1      ACAAAGGGTCCGACAGTACCAGATGAAGACTTTTTCAGCCTTATTTTACGGTCCCAGGGA
NM_013296.4         ACAAAGGGTCCGACAGTACCAGATGAAGACTTTTTCAGCCTTATTTTACGGTCCCAGGGA
                                                                                

HsCD00589607        ------------------------------------------------------------
XM_005270787.1      AAGAGAATGGATGAACAGAGAGTTCTTTTACAAAGAGATCAAAACAGAGACACTGACTTT
NM_013296.4         AAGAGAATGGATGAACAGAGAGTTCTTTTACAAAGAGATCAAAACAGAGACACTGACTTT
                                                                                

HsCD00589607        ------------------------------------------------------------
XM_005270787.1      GGGCTAAAGGACTTTTTGCAAAATAATGCTTTGTTGGAGTTTAAAAATTCAGGGAAAAAA
NM_013296.4         GGGCTAAAGGACTTTTTGCAAAATAATGCTTTGTTGGAGTTTAAAAATTCAGGGAAAAAA
                                                                                

HsCD00589607        ---------------
XM_005270787.1      TCGGCAGACCATTAG
NM_013296.4         TCGGCAGACCATTAG
                                   

Pathways

Panther : Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway
Panther : Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway

Protein

SMART domain : TPR : Tetratricopeptide repeats
SMART domain : GoLoco : LGN motif, putative GEFs specific for G-alpha GTPases
Structural Annotation Wiki : HR5762
UniProt : P81274
HPRD : 06609

Clone

Cloning Information : 627365
Original Clone ID : HR5762-1-557-14.3

Experimental

TargetTrack - Experimental data : HR5762

Organism


TAX_ID : 9606
Species Specific ID: 29899

Genome

Chromosome : 1
Map Location : 1p13.3
Ensembl : ENSG00000121957

Reagents

Labome : LGN-antibody