Gene: |
Gene Symbol:
GPSM2 Gene Name:  G-protein signaling modulator 2 Sequence |
---|---|
Original Clone ID: | HR5762-1-557-14.3 |
Keyword: | targeted domain |
Species: | Homo sapiens |
Type: | cDNA |
Vector Name: | pET15_NESG Format: FUSION |
Source: | Northeast Structural Genomics Consortium |
Description: | None |
Comments: | None |
Mutation/ Discrepancy : |
No / Yes |
Publications: | None |
Authors: |
Northeast Structural Genomics Consortium Rutgers University PSI:Biology |
HsCD00589607 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI/NESG |
Insert sequence: 1680nts
Open reading frame : 15 to 1694 Reference Sequence Alignment ACCATCACAGCCATATGAGAGAAGCCCATTCTTTTCATGTTCGTTACAGAATGGAAGCTT
CTTGCCTAGAGCTGGCCTTGGAAGGGGAACGTCTATGTAAATCAGGAGACTGCCGCGCTG
GCGTGTCATTCTTTGAAGCTGCAGTTCAAGTTGGAACTGAAGACCTAAAAACACTTAGCG
CTATTTACAGCCAGTTGGGCAATGCTTATTTCTATTTGCATGATTATGCCAAAGCATTAG
AATATCACCATCATGATTTAACCCTTGCAAGGACTATTGGAGACCAGCTGGGGGAAGCGA
AAGCTAGTGGTAATCTGGGAAACACCTTAAAAGTTCTTGGGAATTTTGACGAAGCCATAG
TTTGTTGTCAGCGACACCTAGATATTTCCAGAGAGCTTAATGACAAGGTGGGAGAAGCAA
GAGCACTTTACAATCTTGGGAATGTGTATCATGCCAAAGGGAAAAGTTTTGGTTGTCCTG
GTCCCCAGGATGTAGGAGAATTTCCAGAAGAAGTGAGAGATGCTCTGCAGGCAGCCGTGG
ATTTTTATGAGGAAAACCTATCATTAGTGACTGCTTTGGGTGACCGAGCGGCACAAGGAC
GTGCCTTTGGAAATCTTGGAAACACACATTACCTCCTTGGCAACTTCAGGGATGCAGTTA
TAGCTCATGAGCAGCGTCTCCTTATTGCAAAAGAATTTGGAGATAAAGCAGCTGAAAGAA
GAGCATATAGCAACCTTGGAAATGCATATATATTTCTTGGTGAATTTGAAACTGCCTCGG
AATACTACAAGAAGACACTACTGTTGGCCCGACAGCTTAAAGACCGAGCTGTAGAAGCAC
AGTCTTGTTACAGTCTTGGAAATACATATACTTTACTTCAAGACTATGAAAAGGCCATTG
ATTATCATCTGAAGCACTTAGCAATTGCTCAAGAGCTGAATGATAGAATTGGTGAAGGAA
GAGCATGTTGGAGCTTAGGAAATGCATACACAGCACTAGGAAATCATGATCAAGCAATGC
ATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGATAAAAGTGGTGAACTAA
CAGCACGACTTAATCTCTCAGACCTTCAAATGGTTCTTGGTCTGAGCTACAGCACAAATA
ACTCCATAATGTCTGAAAATACTGAAATTGATAGCAGTTTGAATGGTGTACGCCCCAAGT
TGGGACGCCGGCATAGTATGGAAAATATGGAACTTATGAAGTTAACACCAGAAAAGGTAC
AGAACTGGAACAGTGAAATTCTTGCTAAGCAAAAACCTCTTATTGCCAAACCTTCTGCAA
AGCTACTCTTTGTCAACAGACTGAAGGGGAAAAAATACAAAACGAATTCCTCCACTAAAG
TTCTCCAAGATGCCAGTAATTCTATTGACCACCGAATTCCAAATTCTCAGAGGAAAATCA
GTGCAGATACTATTGGAGATGAAGGGTTCTTTGACTTATTAAGCCGATTTCAAAGCAATA
GGATGGATGATCAGAGATGTTGCTTACAAGAAAAGAACTGCCATACAGCTTCAACAACAA
CTTCTTCCACTCCCCCTAAAATGATGCTAAAAACATCATCTGTTCCTGTGGTATCCCCCA
ACACGGATGAGTTTTTAGATCTTCTTGCCAGCTCACAGAGTCGCCGTCTGGATGACCAGA
GGGCTTAGCTCGAG
|
Coding Sequence Details | |
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Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
Mutations: None |
Discrepancy:a11c,D4A;c462t,C154C |
5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT |
|
3' Linker Sequence:
CTCGAGATCCGGCTGC |
Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
---|---|
Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
Protein Expression Results: |
ProteinExpressed: Protein_Confirmed ProteinPurified: Not_Tested SolubleProtein: Protein_Soluble Find experimental protocols in HR5762 |
Recommended expression in: | E. coli BL21 |
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Vector Name: | pET15_NESG |
pET15_NESG in advanced viewed |
Synonyms: | pET15, pET15-modified, pET15Cc, pET15_NESG | |
Sequencing Primer: | Forward:
T7
Reverse: T7 terminator |
|
Description: | Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning. | |
Comments: | Modified Novagen pET vector-common MCS | |
Size (bp): | 5723 | |
Parent Vector: | None | |
Empty Vector: | EvNO00336943 | |
Properties: | bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker | |
Author Name: |
Northeast Structural Genomics Consortium Gaetano T. Montleione Jessica Bearden |
|
Publications: |
PMID:
15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium |
|
Vector Map: |
![]() ![]() |
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Sequence: |
Vector Features:
Type | Name | Description | Start Position | End Position |
---|---|---|---|---|
MCS | MCS | MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) | 327 | 452 |
bacterial origin | ori | ColE1 origin of replication | 3881 | 4563 |
gene fragment | ROP | repressor of primer ROP sequence | 3281 | 3457 |
primer | T7 forward | T7 forward sequencing primer | 481 | 496 |
primer | T7 reverse | T7 reverse sequencing primer | 257 | 275 |
promoter | T7 | T7 promoter | 478 | 497 |
repressor binding site | lacO | lacO regulatory sequence | 456 | 478 |
selectable marker | AmpR | ampicillin resistance gene | 4661 | 5230 |
tag | His | N-terminal 6x His tag | 387 | 404 |
trxn termination sequence | T7 term | T7 termination sequence | 257 | 275 |
- Gene
- Pathways
- Protein
- Clone
- Experimental
- Organism
- Genome
- Reagents
Gene
Gene Symbol : | GPSM2 |
---|---|
Symbol Nomenclature : | GPSM2 |
SYNONYM : | CMCS; DFNB82; LGN; PINS |
Designation : | G-protein signalling modulator 2 (AGS3-like, C. elegans)|G-protein-signaling modulator 2|mosaic protein LGN |
Full Nomenclature : | G-protein signaling modulator 2 |
GENEID : | 29899 |
Locus Tag : | RP11-475E11.4 |
GI : | 164519122 |
GenBank Accession : | NM_013296 |
HGNC : | 29501 |
MIM : | 609245 |
Target GenBank: | None |
Reference Sequence Alignment
HsCD00589607 -------ACCATCACAGCCATATGAGAGAAGCCCATTCTTTTCATGTTCGTTACAGAATG XM_005270787.1 ATGGAGGAAAATTTGATAAGCATGAGAGAAGACCATTCTTTTCATGTTCGTTACAGAATG NM_013296.4 ATGGAGGAAAATTTGATAAGCATGAGAGAAGACCATTCTTTTCATGTTCGTTACAGAATG *..** : * ... **********.**************************** HsCD00589607 GAAGCTTCTTGCCTAGAGCTGGCCTTGGAAGGGGAACGTCTATGTAAATCAGGAGACTGC XM_005270787.1 GAAGCTTCTTGCCTAGAGCTGGCCTTGGAAGGGGAACGTCTATGTAAATCAGGAGACTGC NM_013296.4 GAAGCTTCTTGCCTAGAGCTGGCCTTGGAAGGGGAACGTCTATGTAAATCAGGAGACTGC ************************************************************ HsCD00589607 CGCGCTGGCGTGTCATTCTTTGAAGCTGCAGTTCAAGTTGGAACTGAAGACCTAAAAACA XM_005270787.1 CGCGCTGGCGTGTCATTCTTTGAAGCTGCAGTTCAAGTTGGAACTGAAGACCTAAAAACA NM_013296.4 CGCGCTGGCGTGTCATTCTTTGAAGCTGCAGTTCAAGTTGGAACTGAAGACCTAAAAACA ************************************************************ HsCD00589607 CTTAGCGCTATTTACAGCCAGTTGGGCAATGCTTATTTCTATTTGCATGATTATGCCAAA XM_005270787.1 CTTAGCGCTATTTACAGCCAGTTGGGCAATGCTTATTTCTATTTGCATGATTATGCCAAA NM_013296.4 CTTAGCGCTATTTACAGCCAGTTGGGCAATGCTTATTTCTATTTGCATGATTATGCCAAA ************************************************************ HsCD00589607 GCATTAGAATATCACCATCATGATTTAACCCTTGCAAGGACTATTGGAGACCAGCTGGGG XM_005270787.1 GCATTAGAATATCACCATCATGATTTAACCCTTGCAAGGACTATTGGAGACCAGCTGGGG NM_013296.4 GCATTAGAATATCACCATCATGATTTAACCCTTGCAAGGACTATTGGAGACCAGCTGGGG ************************************************************ HsCD00589607 GAAGCGAAAGCTAGTGGTAATCTGGGAAACACCTTAAAAGTTCTTGGGAATTTTGACGAA XM_005270787.1 GAAGCGAAAGCTAGTGGTAATCTGGGAAACACCTTAAAAGTTCTTGGGAATTTTGACGAA NM_013296.4 GAAGCGAAAGCTAGTGGTAATCTGGGAAACACCTTAAAAGTTCTTGGGAATTTTGACGAA ************************************************************ HsCD00589607 GCCATAGTTTGTTGTCAGCGACACCTAGATATTTCCAGAGAGCTTAATGACAAGGTGGGA XM_005270787.1 GCCATAGTTTGTTGTCAGCGACACCTAGATATTTCCAGAGAGCTTAATGACAAGGTGGGA NM_013296.4 GCCATAGTTTGTTGTCAGCGACACCTAGATATTTCCAGAGAGCTTAATGACAAGGTGGGA ************************************************************ HsCD00589607 GAAGCAAGAGCACTTTACAATCTTGGGAATGTGTATCATGCCAAAGGGAAAAGTTTTGGT XM_005270787.1 GAAGCAAGAGCACTTTACAATCTTGGGAATGTGTATCATGCCAAAGGGAAAAGTTTTGGT NM_013296.4 GAAGCAAGAGCACTTTACAATCTTGGGAATGTGTATCATGCCAAAGGGAAAAGTTTTGGT ************************************************************ HsCD00589607 TGTCCTGGTCCCCAGGATGTAGGAGAATTTCCAGAAGAAGTGAGAGATGCTCTGCAGGCA XM_005270787.1 TGCCCTGGTCCCCAGGATGTAGGAGAATTTCCAGAAGAAGTGAGAGATGCTCTGCAGGCA NM_013296.4 TGCCCTGGTCCCCAGGATGTAGGAGAATTTCCAGAAGAAGTGAGAGATGCTCTGCAGGCA ** ********************************************************* HsCD00589607 GCCGTGGATTTTTATGAGGAAAACCTATCATTAGTGACTGCTTTGGGTGACCGAGCGGCA XM_005270787.1 GCCGTGGATTTTTATGAGGAAAACCTATCATTAGTGACTGCTTTGGGTGACCGAGCGGCA NM_013296.4 GCCGTGGATTTTTATGAGGAAAACCTATCATTAGTGACTGCTTTGGGTGACCGAGCGGCA ************************************************************ HsCD00589607 CAAGGACGTGCCTTTGGAAATCTTGGAAACACACATTACCTCCTTGGCAACTTCAGGGAT XM_005270787.1 CAAGGACGTGCCTTTGGAAATCTTGGAAACACACATTACCTCCTTGGCAACTTCAGGGAT NM_013296.4 CAAGGACGTGCCTTTGGAAATCTTGGAAACACACATTACCTCCTTGGCAACTTCAGGGAT ************************************************************ HsCD00589607 GCAGTTATAGCTCATGAGCAGCGTCTCCTTATTGCAAAAGAATTTGGAGATAAAGCAGCT XM_005270787.1 GCAGTTATAGCTCATGAGCAGCGTCTCCTTATTGCAAAAGAATTTGGAGATAAAGCAGCT NM_013296.4 GCAGTTATAGCTCATGAGCAGCGTCTCCTTATTGCAAAAGAATTTGGAGATAAAGCAGCT ************************************************************ HsCD00589607 GAAAGAAGAGCATATAGCAACCTTGGAAATGCATATATATTTCTTGGTGAATTTGAAACT XM_005270787.1 GAAAGAAGAGCATATAGCAACCTTGGAAATGCATATATATTTCTTGGTGAATTTGAAACT NM_013296.4 GAAAGAAGAGCATATAGCAACCTTGGAAATGCATATATATTTCTTGGTGAATTTGAAACT ************************************************************ HsCD00589607 GCCTCGGAATACTACAAGAAGACACTACTGTTGGCCCGACAGCTTAAAGACCGAGCTGTA XM_005270787.1 GCCTCGGAATACTACAAGAAGACACTACTGTTGGCCCGACAGCTTAAAGACCGAGCTGTA NM_013296.4 GCCTCGGAATACTACAAGAAGACACTACTGTTGGCCCGACAGCTTAAAGACCGAGCTGTA ************************************************************ HsCD00589607 GAAGCACAGTCTTGTTACAGTCTTGGAAATACATATACTTTACTTCAAGACTATGAAAAG XM_005270787.1 GAAGCACAGTCTTGTTACAGTCTTGGAAATACATATACTTTACTTCAAGACTATGAAAAG NM_013296.4 GAAGCACAGTCTTGTTACAGTCTTGGAAATACATATACTTTACTTCAAGACTATGAAAAG ************************************************************ HsCD00589607 GCCATTGATTATCATCTGAAGCACTTAGCAATTGCTCAAGAGCTGAATGATAGAATTGGT XM_005270787.1 GCCATTGATTATCATCTGAAGCACTTAGCAATTGCTCAAGAGCTGAATGATAGAATTGGT NM_013296.4 GCCATTGATTATCATCTGAAGCACTTAGCAATTGCTCAAGAGCTGAATGATAGAATTGGT ************************************************************ HsCD00589607 GAAGGAAGAGCATGTTGGAGCTTAGGAAATGCATACACAGCACTAGGAAATCATGATCAA XM_005270787.1 GAAGGAAGAGCATGTTGGAGCTTAGGAAATGCATACACAGCACTAGGAAATCATGATCAA NM_013296.4 GAAGGAAGAGCATGTTGGAGCTTAGGAAATGCATACACAGCACTAGGAAATCATGATCAA ************************************************************ HsCD00589607 GCAATGCATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGATAAAAGTGGT XM_005270787.1 GCAATGCATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGATAAAAGTGGT NM_013296.4 GCAATGCATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGATAAAAGTGGT ************************************************************ HsCD00589607 GAACTAACAGCACGACTTAATCTCTCAGACCTTCAAATGGTTCTTGGTCTGAGCTACAGC XM_005270787.1 GAACTAACAGCACGACTTAATCTCTCAGACCTTCAAATGGTTCTTGGTCTGAGCTACAGC NM_013296.4 GAACTAACAGCACGACTTAATCTCTCAGACCTTCAAATGGTTCTTGGTCTGAGCTACAGC ************************************************************ HsCD00589607 ACAAATAACTCCATAATGTCTGAAAATACTGAAATTGATAGCAGTTTGAATGGTGTACGC XM_005270787.1 ACAAATAACTCCATAATGTCTGAAAATACTGAAATTGATAGCAGTTTGAATGGTGTACGC NM_013296.4 ACAAATAACTCCATAATGTCTGAAAATACTGAAATTGATAGCAGTTTGAATGGTGTACGC ************************************************************ HsCD00589607 CCCAAGTTGGGACGCCGGCATAGTATGGAAAATATGGAACTTATGAAGTTAACACCAGAA XM_005270787.1 CCCAAGTTGGGACGCCGGCATAGTATGGAAAATATGGAACTTATGAAGTTAACACCAGAA NM_013296.4 CCCAAGTTGGGACGCCGGCATAGTATGGAAAATATGGAACTTATGAAGTTAACACCAGAA ************************************************************ HsCD00589607 AAGGTACAGAACTGGAACAGTGAAATTCTTGCTAAGCAAAAACCTCTTATTGCCAAACCT XM_005270787.1 AAGGTACAGAACTGGAACAGTGAAATTCTTGCTAAGCAAAAACCTCTTATTGCCAAACCT NM_013296.4 AAGGTACAGAACTGGAACAGTGAAATTCTTGCTAAGCAAAAACCTCTTATTGCCAAACCT ************************************************************ HsCD00589607 TCTGCAAAGCTACTCTTTGTCAACAGACTGAAGGGGAAAAAATACAAAACGAATTCCTCC XM_005270787.1 TCTGCAAAGCTACTCTTTGTCAACAGACTGAAGGGGAAAAAATACAAAACGAATTCCTCC NM_013296.4 TCTGCAAAGCTACTCTTTGTCAACAGACTGAAGGGGAAAAAATACAAAACGAATTCCTCC ************************************************************ HsCD00589607 ACTAAAGTTCTCCAAGATGCCAGTAATTCTATTGACCACCGAATTCCAAATTCTCAGAGG XM_005270787.1 ACTAAAGTTCTCCAAGATGCCAGTAATTCTATTGACCACCGAATTCCAAATTCTCAGAGG NM_013296.4 ACTAAAGTTCTCCAAGATGCCAGTAATTCTATTGACCACCGAATTCCAAATTCTCAGAGG ************************************************************ HsCD00589607 AAAATCAGTGCAGATACTATTGGAGATGAAGGGTTCTTTGACTTATTAAGCCGATTTCAA XM_005270787.1 AAAATCAGTGCAGATACTATTGGAGATGAAGGGTTCTTTGACTTATTAAGCCGATTTCAA NM_013296.4 AAAATCAGTGCAGATACTATTGGAGATGAAGGGTTCTTTGACTTATTAAGCCGATTTCAA ************************************************************ HsCD00589607 AGCAATAGGATGGATGATCAGAGATGTTGCTTACAAGAAAAGAACTGCCATACAGCTTCA XM_005270787.1 AGCAATAGGATGGATGATCAGAGATGTTGCTTACAAGAAAAGAACTGCCATACAGCTTCA NM_013296.4 AGCAATAGGATGGATGATCAGAGATGTTGCTTACAAGAAAAGAACTGCCATACAGCTTCA ************************************************************ HsCD00589607 ACAACAACTTCTTCCACTCCCCCTAAAATGATGCTAAAAACATCATCTGTTCCTGTGGTA XM_005270787.1 ACAACAACTTCTTCCACTCCCCCTAAAATGATGCTAAAAACATCATCTGTTCCTGTGGTA NM_013296.4 ACAACAACTTCTTCCACTCCCCCTAAAATGATGCTAAAAACATCATCTGTTCCTGTGGTA ************************************************************ HsCD00589607 TCCCCCAACACGGATGAGTTTTTAGATCTTCTTGCCAGCTCACAGAGTCGCCGTCTGGAT XM_005270787.1 TCCCCCAACACGGATGAGTTTTTAGATCTTCTTGCCAGCTCACAGAGTCGCCGTCTGGAT NM_013296.4 TCCCCCAACACGGATGAGTTTTTAGATCTTCTTGCCAGCTCACAGAGTCGCCGTCTGGAT ************************************************************ HsCD00589607 GACCAGAGGGCTTAGCTCGAG--------------------------------------- XM_005270787.1 GACCAGAGGGCTAGTTTCAGTAATTTGCCAGGGCTTCGTCTAACACAAAACAGCCAGTCG NM_013296.4 GACCAGAGGGCTAGTTTCAGTAATTTGCCAGGGCTTCGTCTAACACAAAACAGCCAGTCG ************:. **.. HsCD00589607 ------------------------------------------------------------ XM_005270787.1 GTACTTAGCCACCTGATGACTAATGACAACAAAGAGGCTGATGAAGATTTCTTTGACATC NM_013296.4 GTACTTAGCCACCTGATGACTAATGACAACAAAGAGGCTGATGAAGATTTCTTTGACATC HsCD00589607 ------------------------------------------------------------ XM_005270787.1 CTTGTAAAATGTCAAGGATCCAGATTAGATGATCAAAGATGTGCTCCACCACCTGCTACC NM_013296.4 CTTGTAAAATGTCAAGGATCCAGATTAGATGATCAAAGATGTGCTCCACCACCTGCTACC HsCD00589607 ------------------------------------------------------------ XM_005270787.1 ACAAAGGGTCCGACAGTACCAGATGAAGACTTTTTCAGCCTTATTTTACGGTCCCAGGGA NM_013296.4 ACAAAGGGTCCGACAGTACCAGATGAAGACTTTTTCAGCCTTATTTTACGGTCCCAGGGA HsCD00589607 ------------------------------------------------------------ XM_005270787.1 AAGAGAATGGATGAACAGAGAGTTCTTTTACAAAGAGATCAAAACAGAGACACTGACTTT NM_013296.4 AAGAGAATGGATGAACAGAGAGTTCTTTTACAAAGAGATCAAAACAGAGACACTGACTTT HsCD00589607 ------------------------------------------------------------ XM_005270787.1 GGGCTAAAGGACTTTTTGCAAAATAATGCTTTGTTGGAGTTTAAAAATTCAGGGAAAAAA NM_013296.4 GGGCTAAAGGACTTTTTGCAAAATAATGCTTTGTTGGAGTTTAAAAATTCAGGGAAAAAA HsCD00589607 --------------- XM_005270787.1 TCGGCAGACCATTAG NM_013296.4 TCGGCAGACCATTAG
Pathways
Panther : | Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway |
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Panther : | Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway |
Protein
SMART domain : | GoLoco : LGN motif, putative GEFs specific for G-alpha GTPases |
---|---|
SMART domain : | TPR : Tetratricopeptide repeats |
Structural Annotation Wiki : | HR5762 |
UniProt : | P81274 |
HPRD : | 06609 |
Clone
Cloning Information : | 627365 |
---|---|
Original Clone ID : | HR5762-1-557-14.3 |
Experimental
TargetTrack - Experimental data : | HR5762 |
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Genome
Chromosome : | 1 |
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Map Location : | 1p13.3 |
Ensembl : | ENSG00000121957 |
Reagents
Labome : | LGN-antibody |
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