MLYCD (R. palustris )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MLYCD
Gene Name:  malonyl-CoA decarboxylase
Original Clone ID:
RpR127-8-451-21.13
Keyword:
full length protein truncation
Species:
Rhodopseudomonas palustris
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 23791943
Title: Crystal structures of malonyl-coenzyme a decarboxylase provide insights into its catalytic mechanism and disease-causing mutations.
Authors:
PSI
Northeast Structural Genomics Consortium
RpCD00591508
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1335nts
Open reading frame: 31 to 1365

TTTTGTTAACTTTAAGAAGGAGATATACATATGACCACTGCAGACCGCGCTTCGGAATTT
CTCGGCGGCTTGTTCAACAGCCTCACCGAACGCGGCCGCAGCCTGTTCGGGCTGACCAGC
TCACAGCCGATGTCCGGGGACGAACTGATCGCGCTTAGTGAGACGCTGCTGTCGCGGCGG
GGTGAAGCGTCCGGCGTCGCGCTGGCCGCATCGCTGCTCGCCGGCTACGAGGCTGCCGAC
GAAGACGACAAGCTGGCGTTTCTCGATGCGCTCGCCGAACAGTTCGGGCCCGACCTCGCC
GAACTCAACACCGCGATCGAGGCGTTTCGCGCCGACGCCTCGGCCGAGGCGACCGGTGAA
TTGCTACGCGCCGCCGAGCCGCGCCGGCAGGAGCTGATCCGCCGCCTCAATCACGCTCCG
GGCGGCACCGCGGCGCTGGTGAAGATGCGCGAGGCGGTGCTCGCCCGCATCGCTGCGCAT
CCGCAGCTTCGTCACGTCGACGACGATTTCGTGCATCTGTTCACGTCGTGGTTCAATCGG
GGATTTCTCGTGCTGCAGCGGATCGACTGGACGACGCCGGCCAACATCCTGGAAAAGATC
ATCCGCTACGAGCAGGTACACACCATCCACGACTGGGACGATCTGCGCGCGCGGCTGGCC
CCGCCGGACCGGCGTTGCTACGGCTTCTTCCATCCGCGGCTGGTCGACGAGCCGTTGATC
TTCGTCGAGGTCGCGCTGACCAAGGACAGCCCTGCCGCGATCGCACCGCTGCTCGATCTC
GAGCGCGAGCCGATCGCCGCCAGCGATGCAACGACTGCAGTGTTCTATTCGATCTCCAAC
ACCCAGCAGGGCCTCGCCGGGATCTCGTTCGGCAATTTCCTGATCAAGCAGGTGGTGGAG
GAGATCAAGCGCGAGCTGCCGAACGTGCAAACCTTCGTGACGCTGTCGCCGGTGCCGGGC
TTCGCCAAGTGGCTGAAGCGCGAACGCGACAATCCGGATTCGACGCTGCTCGATGCCTCC
GCGCGCACCGCGCTCGAAGCGCTCGATACGCCGAACTGGTTCGACGATGCCGACACCGCC
GACCGGCTGAAGCCGATCGTGCTGCAGCTCGCCGCCGCTTACTTCCTGCAGGCCAAGGGG
CCGAACGGCCGGCCGCTTGATCCGGTGGCCCGCTTCCACCTCGGCAACGGCGCGCGGCTC
GACCGGCTGAACTTCCTCGGCGACCGTTCGCCGAACGGCATGCGCCAGTCGCACGGGCTG
ATGGTCAACTACCTGTATGCGCTCGGCGACATCGAGGCCAACCACGAGGCGCTGTTCGAA
CGCGGCCAGATCGCCGCCGCCTCCGCAGTGCGCAAGCTGGTGCCGC

Protein sequence predicted based on nucleotide sequence

MTTADRASEFLGGLFNSLTERGRSLFGLTSSQPMSGDELIALSETLLSRRGEASGVALAA
SLLAGYEAADEDDKLAFLDALAEQFGPDLAELNTAIEAFRADASAEATGELLRAAEPRRQ
ELIRRLNHAPGGTAALVKMREAVLARIAAHPQLRHVDDDFVHLFTSWFNRGFLVLQRIDW
TTPANILEKIIRYEQVHTIHDWDDLRARLAPPDRRCYGFFHPRLVDEPLIFVEVALTKDS
PAAIAPLLDLEREPIAASDATTAVFYSISNTQQGLAGISFGNFLIKQVVEEIKRELPNVQ
TFVTLSPVPGFAKWLKRERDNPDSTLLDASARTALEALDTPNWFDDADTADRLKPIVLQL
AAAYFLQAKGPNGRPLDPVARFHLGNGARLDRLNFLGDRSPNGMRQSHGLMVNYLYALGD
IEANHEALFERGQIAAASAVRKLVP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in RpR127
Recommended expression in:
Not Applicable