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FGF13 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF13
Gene Name:  fibroblast growth factor 13
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH140004.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00005930

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 738nts         Open reading frame : 1 to 738
Reference Sequence Alignment

ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGAG
AAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAAA
AACAAGTTAAATGTCTTTTCCCGGGTCAAACTCTTCGGCTCCAAGAAGAGGCGCAGAAGA
AGACCAGAGCCTCAGCTTAAGGGTATAGTTACCAAGCTATACAGCCGACAAGGCTACCAC
TTGCAGCTGCAGGCGGATGGAACCATTGATGGCACCAAAGATGAGGACAGCACTTACACT
CTGTTTAACCTCATCCCTGTGGGTCTGCGAGTGGTGGCTATCCAAGGAGTTCAAACCAAG
CTGTACTTGGCAATGAACAGTGAGGGATACTTGTACACCTCGGAACTTTTCACACCTGAG
TGCAAATTCAAAGAATCAGTGTTTGAAAATTATTATGTGACATATTCATCAATGATATAC
CGTCAGCAGCAGTCAGGCCGAGGGTGGTATCTGGGTCTGAACAAAGAAGGAGAGATCATG
AAAGGCAACCATGTGAAGAAGAACAAGCCTGCAGCTCATTTTCTGCCTAAACCACTGAAA
GTGGCCATGTACAAGGAGCCATCACTGCACGATCTCACGGAGTTCTCCCGATCTGGAAGC
GGGACCCCAACCAAGAGCAGAAGTGTCTCTGGCGTGCTGAACGGAGGCAAATCCATGAGC
CACAATGAATCAACGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1104
Start on reference sequence 367
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF13
Symbol Nomenclature : FGF13
SYNONYM : FGF-13; FGF2; FHF-2; FHF2
Designation : fibroblast growth factor homologous factor 2
Full Nomenclature : fibroblast growth factor 13
GENEID : 2258
Locus Tag : RP6-27P15.3
GI : 61364836
GenBank Accession : AY890669
HGNC : 3670
MIM : 300070
Vega : OTTHUMG00000022532
Target GenBank: BC034340

 

Reference Sequence Alignment

  

HsCD00005930        -ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGA
NM_001139501.1      ------------------------------------------------------------
XM_005262399.1      ATGAGTGGAAAGGTGACCAAGCCCAAAGAGGAGAAAGATGCTTCTAAGGTTCTGGATGAC
NM_004114.3         -ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGA
NM_001139502.1      ------------------------------------------------------------
NM_001139500.1      ATGAGTGGAAAGGTGACCAAGCCCAAAGAGGAGAAAGATGCTTCTAAGGTTCTGGATGAC
                                                                                

HsCD00005930        GAAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAA
NM_001139501.1      ---------------------------ATGTTACGACAAGATTCCATCCAATCTGCGGAA
XM_005262399.1      GCCCCCCCTGGCACACAGGAATACATTATGTTACGACAAGATTCCATCCAATCTGCGGAA
NM_004114.3         GAAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAA
NM_001139502.1      ---------------------------ATGTTACGACAAGATTCCATCCAATCTGCGGAA
NM_001139500.1      GCCCCCCCTGGCACACAGGAATACATTATGTTACGACAAGATTCCATCCAATCTGCGGAA
                                               .:*  .*..***..  ...:***.     . **

HsCD00005930        AAACAAGTTAAATG-----------------------------TCTTTTCCCGGGTCAAA
NM_001139501.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
XM_005262399.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
NM_004114.3         AAACAAGTTAAATG-----------------------------TCTTTTCCCGGGTCAAA
NM_001139502.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
NM_001139500.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
                    ::*.*..::*** .                             **** * * *    .:.

HsCD00005930        CTCTTCGGCTCCAAGAAGAGGCGCAGAAGAAGACCAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139501.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
XM_005262399.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
NM_004114.3         CTCTTCGGCTCCAAGAAGAGGCGCAGAAGAAGACCAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139502.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139500.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
                    .:  :.* . .*.* **...*..** *.... . .*************************

HsCD00005930        ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139501.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
XM_005262399.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_004114.3         ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139502.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139500.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
                    ************************************************************

HsCD00005930        GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139501.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
XM_005262399.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_004114.3         GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139502.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139500.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
                    ************************************************************

HsCD00005930        GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139501.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
XM_005262399.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_004114.3         GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139502.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139500.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
                    ************************************************************

HsCD00005930        TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139501.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
XM_005262399.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_004114.3         TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139502.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139500.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
                    ************************************************************

HsCD00005930        TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139501.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
XM_005262399.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_004114.3         TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139502.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139500.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
                    ************************************************************

HsCD00005930        CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139501.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
XM_005262399.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_004114.3         CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139502.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139500.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
                    ************************************************************

HsCD00005930        GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139501.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
XM_005262399.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_004114.3         GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139502.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139500.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
                    ************************************************************

HsCD00005930        GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139501.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
XM_005262399.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_004114.3         GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139502.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139500.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
                    ************************************************************

HsCD00005930        GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_001139501.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
XM_005262399.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_004114.3         GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_001139502.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_001139500.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
                    ************************************************

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Protein

SMART domain : FGF : Acidic and basic fibroblast growth factor family.
UniProt : B7Z4M7
UniProt : B7Z8N0
UniProt : A8K1P5
UniProt : Q92913
UniProt : D3DWH4
HPRD : 02090

Clone

Cloning Information : 140004
HIP Master Clone ID : 116271
Original Clone ID : FLH140004.01X

Organism


TAX_ID : 9606
Species Specific ID: 2258

Genome

Chromosome : X
Map Location : Xq26.3
Ensembl : ENSG00000129682

Reagents

Labome : FGF13-antibody