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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  TRS1
Sequence             
Original Clone ID: None
Keyword: Antibiotic: Amp; Order: 44; Full Length?: Yes
Species: Human herpesvirus 5
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 26450993
Title: Immunoproteomic Profiling of Antiviral Antibodies in New-Onset Type 1 Diabetes Using Protein Arrays
Authors: Center for Personalized Diagnostics
Xiaofang Bian
HhCD00594847

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2361nts         Open reading frame : 1 to 2361

ATGGCCCAGCGCAACGGCATGTCGCCGCGCCCCCCGCCCCTCGGTCGCGGCCGCGGAGCC
GGAGGGCCTTCGGGGGTTGGTTCCTCTCGTTCTTCTTCTTTGGAAACGACGTCAACAGCG
GGGACTAGTACGAGTGCTGCGGGTACGGCGACACCGGCCCACGCCGTCCACCGGGTAGAA
CCCCGCGGGCCGCCGGGCGCCCCTCCGGGTAGCGGCAACAACACCAACTTTTGGCACGGC
CCGGAGCGCTTGTTGCTGTCTCAGATTCCGGTGGAGCGCCAGGCGCTGACGGAGCTGGAA
TACCAGGCCATGGGCGCCGTGTGGCGCGCGGCGTTTTTGGCCAACAGCACGGGCCGCGCC
ATGCGCAAGTGGTCGCAGCGCGACGCGGGCACGCTGCTGCCGCTCGGACGGCCGTACGGA
TTCTACGCGCGGGTGACGCCGCGCAGCCAGATGAACGGCGTGGGCGCGACGGACCTGCGT
CAGCTGTCGCCGCGGGACGCGTGGATCGTGCTGGTGGCGACCGTGGTGCACGAGGTGGAC
CCCGCGGCCGACCCGACGGTGGGCGACAAGGCCGGCCATCCCGAGGGTCTGTGCGCGCAG
GACGGACTGTACCTGGCGCTGGGCGCCGGGTTCCGCGTGTTCGTGTACGACCTGGCGAAC
AACACGCTGATCCTGGCGGCGCGCGACGCGGACGAGTGGTTTCGGCACGGCGCGGGCGAG
GTGGTGCGGCTGTACCGCTGCAACCGGCTGGGCGTGGGCACCCCGCGCGCGACGCTGCTG
CCTCAGCCGGCGCTTCGCCAGACGTTGCTGCGCGCCGAGGAGGCGACGGCGCTCGGACGG
GAGCTGCGCCGCCGGTGGGCCGGCACGACGGTGGCACTGCAGACGCCGGGCAGGCGACTG
CAGCCGATGGTGCTGCTGGGCGCGTGGCAGGAGCTGGCGCAGTACGAGCCGTTCGCGTCG
GCGCCGCACCCCGCGTCGCTGCTGACGGCCGTGCGTCGGCACCTGAACCAGCGTCTGTGC
TGCGGCTGGCTGGCGCTGGGCGCGGTGCTGCCGTCGCGGTGGCTGGGCTGCGCGGCGGGG
CCGGCGACAGGGACGGCGGCGGGGACGACGTCGCCGCCGGGGGCGAGCGGCACGGAGACG
GAGGCCGCCGGCGGGGACGCGCCGTGCGCGATAGCGGGAGCCGTGGGGTCTGCTGTGACT
TTACCTCCGCAGCCGTACGGCGCCGCCGGCGGGAGCGCGATTTGCGTGCCAAGCGCGGAC
GCGCACGCGGTGGTCGGGGCGGACGCGGCGGCAGCGGCAGCAGCGGCGGCGCCGACGGTG
ATGGTGGGTCCGGCGGCGATGGTGGGTCCGGCGGCGTCGGGGACCGTACCGCGCGCCATG
CTGGTGGTGCTGCTGGACGAGCTGGGCGCCGTGTTCGGGTACTGCCCGCTGGACGGACAC
GTGTACCCGCTGGCGGCGGAGCTGTCGCACTTTCTGCGCGCGGGCGTGCTGGGCGCGCTG
GCGCTGGGGCGCGAGTCGGCGCCCGCCGCCGAGGCCGCGCGGCGGCTGCTGCCCGAGCTG
GACCGCGAGCAGTGGGAGCGGCCGCGCTGGGACGCGCTGCACCTGCACCCGCGCGCCGCG
CTGTGGGCGCGCGAGCCGCACGGGCAGTTAGCTTTTTTGCTACGTCCGGGCCGCGGCGAG
GCTGAGGTGCTCACCCTAGCCACCAAACATCCGGTCATCTGTGCAAATGTGGAAGACTAT
CTGCAAGACGCCAGACGCCGCGCCGACGCTCAAGCTCTGGGACTGGACTTGGCCACGGTG
GTTATGGAAGCCGGTGGCCAGATGATACACAAAAAGACCAAAAAGCCTAAAGGCAAGGAA
GACGAATCGGTCATGAAGGGAAAGCACTCGCGCTACACCCGACCCACAGAACCACCGTTG
ACTCCTCAAGCCAGTTTGGGACGTGCTTTGAGACGAGACGACGAGGATTGGAAACCGTCG
CGTGTCCCTGGAGAAGATTCCTGGTACGACTTGGACGAGACTTTCTGGGTTCTGGGGAGT
AACCGTAAAAACGACGTCTATCAACGACGTTGGAAGAAAACGGTGTTACGCTGTGGTTTG
GAGATTGATCGTCCCATGCCAACGGTCCCCAAGGGCTGCCGCCCGCAGACTTTTACTCAT
GAGGGCATTCAGTTGATGGGTGGTGCTACTCAGGAACCGCTGGATACTGGACTATATGCT
CCGTCTCACGTTACTTCTGCTTTTGTTCCATCTGTGTACATGCCTCCCACCGTACCCTAC
CCCGACCCCGCTGCCAGATTGTGCCGCGACATGCGACGCGTCACGTTTTCTAATGTAGCC
ACGCATTATCATTACAATGCT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

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Cloning Information : 660849