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NTHL1 (Homo sapiens) in pET15_NESG (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NTHL1
Gene Name:  nth endonuclease III-like 1 (E. coli)
Sequence             
Original Clone ID: HR6834-88-312-15.3
Keyword: targeted domain
Species: Homo sapiens
Type: cDNA
Vector Name: pET15_NESG               Format:  CLOSED
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Northeast Structural Genomics Consortium
Rutgers University
PSI:Biology
HsCD00598074

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 681nts         Open reading frame : 51 to 731
Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGAGCCCC
AGGACTGGCAGCAACAGCTGGTCAACATCCGTGCCATGAGGAACAAAAAGGATGCACCTG
TGGACCATCTGGGGACTGAGCACTGCTATGACTCCAGTGCCCCCCCAAAGGTACGCAGGT
ACCAGGTGCTGCTGTCACTGATGCTCTCCAGCCAAACCAAAGACCAGGTGACGGCGGGCG
CCATGCAGCGACTGCGGGCGCGGGGCCTGACGGTGGACAGCATCCTGCAGACAGATGATG
CCACGCTGGGCAAGCTCATCTACCCCGTCGGTTTCTGGAGGAGCAAGGTGAAATACATCA
AGCAGACCAGCGCCATCCTGCAGCAGCACTACGGTGGGGACATCCCAGCCTCTGTGGCCG
AGCTGGTGGCGCTGCCGGGTGTTGGGCCCAAGATGGCACACCTGGCTATGGCTGTGGCCT
GGGGCACTGTGTCAGGCATTGCAGTGGACACGCATGTGCACAGAATCGCCAACAGGCTGA
GGTGGACCAAGAAGGCAACCAAGTCCCCAGAGGAGACCCGCGCCGCCCTGGAGGAGTGGC
TGCCTAGGGAGCTGTGGCACGAGATCAATGGACTCTTGGTGGGCTTCGGCCAGCAGACCT
GTCTGCCTGTGCACCCTCGCTGCCACGCCTGCCTCAACCAAGCCCTCTGCCCGGCCGCCC
AGGGTCTCTAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGC
TG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT

3' Linker Sequence:
CTCGAGATCCGGCTGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in HR6834
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NTHL1
Symbol Nomenclature : NTHL1
SYNONYM : NTH1; OCTS3
Designation : endonuclease III-like protein 1
Full Nomenclature : nth endonuclease III-like 1 (E. coli)
GENEID : 4913
GI : 4505471
GenBank Accession : NM_002528
HGNC : 8028
MIM : 602656
Vega : OTTHUMG00000128744
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00598074      ------------------------------------------------------------
NM_002528.5       ATGTGTAGTCCGCAGGAGTCCGGCATGACCGCCTTGAGCGCGAGGATGCTGACCCGGAGC
                                                                              

HsCD00598074      ------------------------------------------------------------
NM_002528.5       CGGAGCCTGGGACCCGGGGCTGGGCCGCGGGGGTGTAGGGAGGAGCCCGGGCCTCTCCGG
                                                                              

HsCD00598074      ------------------------------------------------------------
NM_002528.5       AGAAGAGAGGCTGCAGCAGAAGCGAGGAAAAGCCACAGCCCCGTGAAGCGTCCGCGGAAA
                                                                              

HsCD00598074      ----------------------------CTTTAAGAAGGAGATATACCATGGGCCATCAC
NM_002528.5       GCACAGAGACTGCGTGTGGCCTATGAGGGCTCGGACAGTGAGAAAGGTGAGGGGGCTGAG
                                                * ....** ...:*:.  .:***  .* * 

HsCD00598074      CATCACCATCACAGCCATATGGAGCCCCAGGACTGGCAGCAACAGCTGGTCAACATCCGT
NM_002528.5       CCCCTCAAGGTGCCAGTCTGGGAGCCCCAGGACTGGCAGCAACAGCTGGTCAACATCCGT
                  *. *:*.*  : . . : : ****************************************

HsCD00598074      GCCATGAGGAACAAAAAGGATGCACCTGTGGACCATCTGGGGACTGAGCACTGCTATGAC
NM_002528.5       GCCATGAGGAACAAAAAGGATGCACCTGTGGACCATCTGGGGACTGAGCACTGCTATGAC
                  ************************************************************

HsCD00598074      TCCAGTGCCCCCCCAAAGGTACGCAGGTACCAGGTGCTGCTGTCACTGATGCTCTCCAGC
NM_002528.5       TCCAGTGCCCCCCCAAAGGTACGCAGGTACCAGGTGCTGCTGTCACTGATGCTCTCCAGC
                  ************************************************************

HsCD00598074      CAAACCAAAGACCAGGTGACGGCGGGCGCCATGCAGCGACTGCGGGCGCGGGGCCTGACG
NM_002528.5       CAAACCAAAGACCAGGTGACGGCGGGCGCCATGCAGCGACTGCGGGCGCGGGGCCTGACG
                  ************************************************************

HsCD00598074      GTGGACAGCATCCTGCAGACAGATGATGCCACGCTGGGCAAGCTCATCTACCCCGTCGGT
NM_002528.5       GTGGACAGCATCCTGCAGACAGATGATGCCACGCTGGGCAAGCTCATCTACCCCGTCGGT
                  ************************************************************

HsCD00598074      TTCTGGAGGAGCAAGGTGAAATACATCAAGCAGACCAGCGCCATCCTGCAGCAGCACTAC
NM_002528.5       TTCTGGAGGAGCAAGGTGAAATACATCAAGCAGACCAGCGCCATCCTGCAGCAGCACTAC
                  ************************************************************

HsCD00598074      GGTGGGGACATCCCAGCCTCTGTGGCCGAGCTGGTGGCGCTGCCGGGTGTTGGGCCCAAG
NM_002528.5       GGTGGGGACATCCCAGCCTCTGTGGCCGAGCTGGTGGCGCTGCCGGGTGTTGGGCCCAAG
                  ************************************************************

HsCD00598074      ATGGCACACCTGGCTATGGCTGTGGCCTGGGGCACTGTGTCAGGCATTGCAGTGGACACG
NM_002528.5       ATGGCACACCTGGCTATGGCTGTGGCCTGGGGCACTGTGTCAGGCATTGCAGTGGACACG
                  ************************************************************

HsCD00598074      CATGTGCACAGAATCGCCAACAGGCTGAGGTGGACCAAGAAGGCAACCAAGTCCCCAGAG
NM_002528.5       CATGTGCACAGAATCGCCAACAGGCTGAGGTGGACCAAGAAGGCAACCAAGTCCCCAGAG
                  ************************************************************

HsCD00598074      GAGACCCGCGCCGCCCTGGAGGAGTGGCTGCCTAGGGAGCTGTGGCACGAGATCAATGGA
NM_002528.5       GAGACCCGCGCCGCCCTGGAGGAGTGGCTGCCTAGGGAGCTGTGGCACGAGATCAATGGA
                  ************************************************************

HsCD00598074      CTCTTGGTGGGCTTCGGCCAGCAGACCTGTCTGCCTGTGCACCCTCGCTGCCACGCCTGC
NM_002528.5       CTCTTGGTGGGCTTCGGCCAGCAGACCTGTCTGCCTGTGCACCCTCGCTGCCACGCCTGC
                  ************************************************************

HsCD00598074      CTCAACCAAGCCCTCTGCCCGGCCGCCCAGGGTCTCTAGCTCGAGATCCGGCTGCTAACA
NM_002528.5       CTCAACCAAGCCCTCTGCCCGGCCGCCCAGGGTCTCTGA---------------------
                  *************************************..                     

HsCD00598074      AAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
NM_002528.5       ------------------------------
                                                

Pathways

Reactome : Base Excision Repair
Reactome : Base-Excision Repair, AP Site Formation
Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome : Cleavage of the damaged pyrimidine
Reactome : DNA Repair
Reactome : Depyrimidination
Reactome : Displacement of DNA glycosylase by APE1
Reactome : Recognition and association of DNA glycosylase with site containing an affected pyrimidine
Reactome : Removal of DNA patch containing abasic residue
Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome : Resolution of AP sites via the single-nucleotide replacement pathway
Reactome : Resolution of Abasic Sites (AP sites)

Protein

Structural Annotation Wiki : HR6834
UniProt : P78549
HPRD : 04039

Clone

Cloning Information : 628921
Original Clone ID : HR6834-88-312-15.3

Experimental

TargetTrack - Experimental data : HR6834

Organism


TAX_ID : 9606
Species Specific ID: 4913

Genome

Chromosome : 16
Map Location : 16p13.3
Ensembl : ENSG00000065057

Reagents

Labome : NTHL1-antibody