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Detailed Vector Information: pMCSG58

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG58              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: None
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00599463

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG58

pMCSG58 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC region plus a portion of the beta-lactamase gene from pMCSG26 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is C-terminal His-tag plasmid with truncated ori and the argU & ileX tRNA genes
Size (bp): 4748
Parent Vector: Name: pMCSG53
Description:
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 2741 3329
ligation independent cloning LIC Ligation independent cloning site 217 238
promoter T7 T7 promoter 300 316
repressor binding site lac operator lac operator 275 297
repressor protein gene LacI LacI cds 1353 2312
selectable marker AmpR ampicillin resistance 3500 4360
tRNA argU argU tRNA gene 383 739
tRNA ileX ileX tRNA gene 899 1050
tag His C-terminal 6xHis tag 208 225
trxn termination sequence T7 term T7 terminator 23 72

 

Species Specific ID: None
Target GenBank: None