| Gene: |
Gene Symbol:
No insert Gene Name: No insert |
|---|---|
| Original Clone ID: | None |
| Type: | No insert |
| Vector Name: | pMCSG58 |
| Source: | Midwest Center for Structural Genomics |
| Description: | Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning |
| Comments: | None |
| Publications: |
PMID: 24057978 Title: New LIC vectors for production of proteins from genes containing rare codons |
| Authors: |
Midwest Center for Structural Genomics PSI:Biology Midwest Center for Structural Genomics |
|
EvNO00599463 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
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| Vector Name: | pMCSG58 |
pMCSG58 in advanced viewed |
| Synonyms: | '' | |
| Sequencing Primer: | Forward:
T7
Reverse: T7 terminator |
|
| Description: | Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning | |
| Comments: | LIC region plus a portion of the beta-lactamase gene from pMCSG26 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is C-terminal His-tag plasmid with truncated ori and the argU & ileX tRNA genes | |
| Size (bp): | 4748 | |
| Parent Vector: |
Name:
pMCSG53 Description: |
|
| Properties: | bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker | |
| Author Name: |
Midwest Center for Structural Genomics PSI:Biology Midwest Center for Structural Genomics |
|
| Publications: |
PMID:
24057978
Title: New LIC vectors for production of proteins from genes containing rare codons |
|
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | pBR322 origin | pBR322 origin of replication | 2741 | 3329 |
| ligation independent cloning | LIC | Ligation independent cloning site | 217 | 238 |
| promoter | T7 | T7 promoter | 300 | 316 |
| repressor binding site | lac operator | lac operator | 275 | 297 |
| repressor protein gene | LacI | LacI cds | 1353 | 2312 |
| selectable marker | AmpR | ampicillin resistance | 3500 | 4360 |
| tRNA | ileX | ileX tRNA gene | 899 | 1050 |
| tRNA | argU | argU tRNA gene | 383 | 739 |
| tag | His | C-terminal 6xHis tag | 208 | 225 |
| trxn termination sequence | T7 term | T7 terminator | 23 | 72 |
| Species Specific ID: | None |
|---|---|
| Target GenBank: | None |
