Bacterial expression vector with T7 promoter and N-terminal TEV-cleavable AviTag and BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
None
Publications:
PMID:24057978 Title: New LIC vectors for production of proteins from genes containing rare codons
Authors:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Bacterial expression vector with T7 promoter and N-terminal TEV-cleavable AviTag and BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC region, BirA gene and a portion of the beta-lactamase gene from pMCSG51 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is biotinylation vector with truncated
ori and the argU & ileX tRNA genes