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Detailed Vector Information: pMCSG62

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG62              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter and N-terminal TEV-cleavable AviTag and BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: None
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00599483

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG62

pMCSG62 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and N-terminal TEV-cleavable AviTag and BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC region, BirA gene and a portion of the beta-lactamase gene from pMCSG51 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is biotinylation vector with truncated ori and the argU & ileX tRNA genes
Size (bp): 5869
Parent Vector: Name: pMCSG53
Description:
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin 3862 4450
gene fragment BirA This protein activates biotin and transfers the biotin moiety to biotin-accepting proteins 244 1206
ligation independent cloning LIC pMCSG7 LIC site 1232 1263
promoter T7 T7 promoter 1421 1437
protease cleavage site TEV TEV protease cleavage site to remove 6xHis AviTag 1251 1271
repressor binding site lac operator lac operator 1396 1418
repressor protein gene LacI Lac I 2474 3433
ribosome binding site RBS Ribosome binding site 1215 1220
selectable marker AmpR Ampicillin resistance 4621 5481
signal sequence biotinylation biotinylation signal 1278 1322
tRNA ileX ileX rare tRNA 2020 2171
tRNA argU argU rare tRNA 1504 1860
tag AviTag N-terminal AviTag 1278 1322
tag His N-terminal 6xHis tag 1329 1346
trxn regulatory element T7 term T7 terminator 23 72

 

Species Specific ID: None
Target GenBank: None