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Detailed Vector Information: pMCSG70

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG70              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: None
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00599485

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG70

pMCSG70 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC region plus a portion of the beta-lactamase gene from pMCSG29 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is pMCSG29 with truncated ori and the argU & ileX tRNA genes
Size (bp): 5911
Parent Vector: Name: pMCSG53
Description:
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
3pUTR 2 micron ori 0 0
bacterial origin pBR322 origin pBR322 bacterial origin 3904 4492
ligation independent cloning LIC ligation independent cloning site 1382 1400
promoter T7 T7 promoter 1463 1479
protease cleavage site TEV TEV protease cleavage site 1368 1388
protease cleavage site TVMV TVMV protease cleavage site 1311 1331
repressor binding site lac operator lac operator 1438 1460
repressor protein gene LacI LacI 2516 3475
selectable marker AmpR Ampicillin resistance 4663 5523
tRNA argU argU rare tRNA 1546 1902
tRNA ileX ileX rare tRNA 2026 2213
tag MBP C-terminal maltose binding protein tag 204 1310
tag His C-terminal 10xHis tag 1332 1361

 

Species Specific ID: None
Target GenBank: None