DNASU Plasmid Repository • 480.965.5697 | Email

Detailed Vector Information: pMCSG71

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG71              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter, N-terminal TVMV-cleavable MBP tag and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: None
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00599486

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pMCSG71

pMCSG71 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter, N-terminal TVMV-cleavable MBP tag and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC region plus a portion of the beta-lactamase gene from pMCSG32 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is pMCSG32 with truncated ori and the argU & ileX tRNA genes
Size (bp): 5940
Parent Vector: Name: pMCSG53
Description:
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 3933 4521
ligation independent cloning LIC ligation independent cloning site 245 265
promoter T7 T7 promoter 1492 1508
protease cleavage site TEV TEV protease cleavage site 232 252
protease cleavage site TVMV TVMV protease cleavage site 263 283
repressor binding site lac operator lac operator 1476 1489
repressor protein gene LacI LacI 2545 2504
selectable marker AmpR ampicilin resistance 4692 5552
tRNA argU argU rare tRNA 1575 1931
tRNA ileX ileX rare tRNA 2091 2242
tag MBP N-terminal Maltose binding protein tag 314 1420
tag His C-terminal 6xHis tag 208 225

 

Species Specific ID: None
Target GenBank: None