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Detailed Vector Information: pMCSG75

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG75              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter and TEV-cleavable 6xHis tag BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: None
Publications: None
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00599503

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pMCSG75

pMCSG75 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and TEV-cleavable 6xHis tag BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC region of pMCSG62 replaced by LIC region of pMCSG28 with the addition of the biotinylation site. Product is a C-terminal biotinylation vector.
Size (bp): 5876
Parent Vector: Name: pMCSG62
Description:
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3851 4533
gene fragment BirA This protein activates biotin and transfers the biotin moiety to biotin-accepting proteins 241 1206
ligation independent cloning LIC ligation independent cloning site 1346 1366
promoter T7 T7 promoter 1428 1444
protease cleavage site TEV TEV protease cleavage site 1333 1353
repressor protein gene LacI LacI 2481 3440
ribosome binding site RBS ribosome binding site 1215 1220
selectable marker AmpR ampicillin resistance 4628 5488
signal sequence biotinylation biotinylation signal 1282 1326
tRNA ileX ileX rare tRNA 2027 2178
tRNA argU argU rare tRNA 1511 1867
tag His 6xHis tag 1258 1275
trxn termination sequence T7 term T7 terminator 26 72

 

Species Specific ID: None
Target GenBank: None