Detailed Vector Information: pMCSG77

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pMCSG77
Source:
Midwest Center for Structural Genomics
Description:
Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; kanamycin resistance in bacteria; ligation independent cloning
Comments:
None
Publications:
PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
EvNO00599523
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: chloramphenicol, ampicillin
Growth Condition: LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin
Comments: None