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Detailed Vector Information: pMCSG77

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG77              
Source: Midwest Center for Structural Genomics
Description: Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; kanamycin resistance in bacteria; ligation independent cloning
Comments: None
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00599523

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  chloramphenicol, ampicillin
Growth Condition:  LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin
Comments:  None
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Vector Name: pMCSG77

pMCSG77 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; kanamycin resistance in bacteria; ligation independent cloning
Comments: None
Size (bp): 5445
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin p15A origin p15A origin 4493 5322
ligation independent cloning LIC MCSG7 LIC site 260 291
ligation independent cloning LIC2 MCSG26 LIC site 223 244
promoter T7 T7 promoter 416 432
protease cleavage site TEV TEV protease cleavage site 279 299
repressor binding site lac operator lac operator 391 413
repressor protein gene LacI LacI 1326 2405
ribosome binding site RBS2 ribosome binding site 353 358
ribosome binding site RBS ribosome binding site 243 248
selectable marker KanR kanamycin resistance 3350 4165
tRNA ileX ileX rare tRNA 1015 1166
tRNA argU argU rare tRNA 499 855
tag His N-terminal 6xHis tag 234 241
trxn termination sequence T7 term T7 terminator 26 72

 

Species Specific ID: None
Target GenBank: None