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recF (Pseudomonas aeruginosa) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  recF
Gene Name:  recombination protein F
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH037996.01F
Keyword: None
Species: Pseudomonas aeruginosa
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
PaCD00006005

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1110nts         Open reading frame : 1 to 1110

ATGTCCCTGACCCGCGTTTCGGTCACCGCGGTGCGCAACCTGCACCCGGTGACCCTCTCC
CCCTCCCCCCGCATCAACATCCTCTACGGCGACAACGGCAGCGGCAAGACCAGCGTGCTC
GAAGCCATCCACCTGCTGGGCCTGGCGCGTTCATTCCGCAGTGCGCGCTTGCAGCCGGTG
GTCCAGTATGAGGAAGCGGCCTGCACCGTATTCGGCCAGGTGATGTTGGCCAACGGCATC
GCCAGCAACCTGGGGATTTCCCGTGAGCGCCAGGGCGAGTTCACCATCCGCATCGATGGG
CAGAACGCCCGGAGTGCGGCTCAATTGGCGGAAACTCTCCCACTGCAACTGATCAACCCG
GACAGCTTTCGGTTGCTCGAGGGAGCGCCGAAGATCCGGCGACAGTTCCTCGATTGGGGA
GTGTTCCACGTGGAACCTCGGTTTCTGCCCGTCTGGCAGCGCCTGCAGAAGGCGCTGCGC
CAGCGGAACTCCTGGCTCCGGCATGGTAAACTGGACCCCGCGTCGCAAGCGGCCTGGGAC
CGGGAATTGAGCCTGGCCAGCGATGAGATCGATGCCTACCGCAGAAGCTATATCCAGGCG
TTGAAACCGGTATTCGAGGAAACACTCGCCGAATTGGTTTCACTGGATGACCTGACCCTT
AGCTACTACCGAGGCTGGGACAAGGACCGGGACCTCCTGGAGGTTCTGGCTTCCAGCCTG
TTGCGCGACCAGCAGATGGGCCACACCCAGGCGGGACCGCAGCGTGCGGATCTTCGCATA
CGGTTGGCAGGTCATAACGCCGCGGAGATTCTCTCGCGCGGTCAGCAGAAGCTGGTGGTA
TGCGCCCTGCGCATCGCCCAAGGCCATCTGATCAATCGCGCCAAGCGCGGACAGTGCGTC
TACCTGGTGGACGACCTGCCCTCGGAACTGGATGAGCAGCATCGAATGGCTCTTTGCCGC
TTGCTTGAAGATTTGGGTTGCCAGGTATTCATCACCTGCGTGGACCCGCAACTATTGAAA
GACGGCTGGCGCACGGATACGCCGGTATCCATGTTCCACGTGGAACATGGAAAAGTCTCT
CAGACCACGACCATCGGGAGTGAAGCAGGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1110
Start on reference sequence 1
Mutations:
None
Discrepancy:
a181g,I61V
                                

5' Linker Sequence:
5p ACAAGTTTGTACAAAAAAGCAGGCTCCGAAGGAGATACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Reagents

Gene

 

Gene Symbol : recF
GENEID : 879229
Locus Tag : PA0003
GI : 49086131
GenBank Accession : AY659055
Pseudomonas aeruginosa (PA) number : PA0003
Target GenBank: NP_064723

 

Protein

UniProt : Q9I7C3

Clone

Cloning Information : 37996
HIP Master Clone ID : 20377
Original Clone ID : FLH037996.01F

Organism


TAX_ID : 208964
Species Specific ID: 879229

Reagents

Labome : recF-cDNA