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Detailed Vector Information: pLIFE-3'UTR

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pLIFE-3'UTR              
Source: Mangone Laboratory
Description: Mammalian expression vector for detecting the expression of 3'UTRs by Firefly luciferase; also contains Renilla luciferase for normalization; ampicillin resistance in bacteria; Gateway cloning with P2RP3 Gateway Cassette
Comments: None
Publications: PMID: 25074381
Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput
PMID: 26645212
Title: A human 3_UTR clone collection to study post-transcriptional gene regulation
Authors: Center for Personalized Diagnostics
Arizona State University
Marco Mangone
Justin Wolter
EvNO00601503

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AVAILABLE
No restriction
Special MTA: SPTA

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pLIFE-3'UTR

pLIFE-3'UTR in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Mammalian expression vector for detecting the expression of 3'UTRs by Firefly luciferase; also contains Renilla luciferase for normalization; ampicillin resistance in bacteria; Gateway cloning with P2RP3 Gateway Cassette
Comments: Growth before gateway recombination requires DB3.1 E. coli cells.
Size (bp): 9206
Parent Vector: None
Properties: Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning
Author Name: Center for Personalized Diagnostics
Arizona State University
Marco Mangone
Justin Wolter
Publications: PMID: 25074381
Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
3pUTR SV40 SV40 3'UTR 3692 3813
bacterial origin ColE ColE1 bacterial origin 1117 1705
binding site MS2 N-terminal 4xMS2 repeat 6038 6204
fluorescent marker Renilla Luciferase Renilla Luciferase 2745 3680
fluorescent marker Firefly Luciferase Firefly luciferase 4375 6027
promoter CMV CMV promoter driving the expression of the Renilla luciferase 2141 2649
promoter PGK PGK promoter driving the expression of the Firefly luciferase and 3'UTR 3854 4352
selectable marker AmpR ampicillin resistance 1 946
selectable marker CmR Chloramphenicol resistance (part of the P2RP3 Gateway Cassette); removed in plasmids containing a 3'UTR 6725 7381
ssDNA origin f1 f1 origin 8706 9161

 

Species Specific ID: None
Target GenBank: None