Mammalian expression vector for detecting the expression of 3'UTRs by Firefly luciferase; also contains Renilla luciferase for normalization; ampicillin resistance in bacteria; Gateway cloning with P2RP3 Gateway Cassette
Comments:
None
Publications:
PMID:25074381 Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput PMID:26645212 Title: A human 3_UTR clone collection to study post-transcriptional gene regulation
Authors:
Arizona State University
Marco Mangone
Justin Wolter
Center for Personalized Diagnostics
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pLIFE-3'UTR
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector for detecting the expression of 3'UTRs by Firefly luciferase; also contains Renilla luciferase for normalization; ampicillin resistance in bacteria; Gateway cloning with P2RP3 Gateway Cassette
Comments:
Growth before gateway recombination requires DB3.1 E. coli cells.