| Gene: |
Gene Symbol:
No insert Gene Name: No insert |
|---|---|
| Original Clone ID: | None |
| Type: | No insert |
| Vector Name: | pLIFE-miRNA |
| Source: | Mangone Laboratory |
| Description: | Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette |
| Comments: | None |
| Publications: |
PMID: 25074381 Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput PMID: 26645212 Title: A human 3_UTR clone collection to study post-transcriptional gene regulation |
| Authors: |
Center for Personalized Diagnostics Arizona State University Marco Mangone Justin Wolter |
|
EvNO00601504 Price: Login for Pricing AVAILABLE No restriction Special MTA: SPTA |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
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| Vector Name: | pLIFE-miRNA |
pLIFE-miRNA in advanced viewed |
| Synonyms: | None | |
| Sequencing Primer: | Forward:
M13F
Reverse: M13R |
|
| Description: | Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette | |
| Comments: | Growth before gateway recombination requires DB3.1 E. coli cells. | |
| Size (bp): | 6500 | |
| Parent Vector: | None | |
| Properties: | Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning | |
| Author Name: |
Center for Personalized Diagnostics Arizona State University Marco Mangone Justin Wolter |
|
| Publications: |
PMID:
25074381
Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput |
|
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| 3pUTR | beta globin | rabbit beta-globin polyadenylation signal | 2484 | 3035 |
| bacterial origin | ColE | ColE1 origin | 4143 | 4731 |
| fluorescent marker | DsRed | mammalian codon-optimized tetrameric variant of DsRed fluorescent protein | 7 | 684 |
| primer | M13 forward | M13 forward sequencing primer | 2412 | 2428 |
| promoter | CMV | CMV promoter | 5925 | 6432 |
| selectable marker | CmR | Chloramphenicol resistance (part of theL2R3 Gateway Cassette); removed in plasmids containing a miRNA | 1516 | 2172 |
| selectable marker | AmpR | ampicillin resistance | 4902 | 5867 |
| splice acceptor site | 3' splice acceptor | 3'splice site | 2435 | 2483 |
| splice donor site | 5' splice donor | 5' splice site | 693 | 701 |
| Species Specific ID: | None |
|---|---|
| Target GenBank: | None |
