| Gene 1: |
Gene Symbol:
Heavy Chain Gene Name: Heavy Chain |
|---|---|
| Gene 2: |
Gene Symbol:
Light Chain Gene Name: Light Chain Sequence |
| Original Clone ID: | anti-TBX5-RAB-S32 |
| Keyword: | anti-TBX5-RAB-S32 recombinant antibody fab fragment |
| Species: | Homo sapiens |
| Type: | cDNA |
| Vector Name: | pBL166 Format: CLOSED |
| Source: | Recombinant Antibody Network |
| Description: | None |
| Comments: | None |
| Mutation/ Discrepancy : |
No / No |
| Publications: | None |
| Authors: |
The University of Chicago Recombinant Antibody Network Protein Capture Reagents Program |
|
HsCD00604331 Price: Login for Pricing AVAILABLE No restriction Special MTA: SPTA |
Insert sequence: 762nts
Open reading frame : 1 to 762 & 1 to 747
ATGAAAAAGAATATCGCATTTCTTCTTGCATCTATGTTCGTTTTTTCTATTGCTACAAAC
GCGTACGCTGAGATCTCCGAGGTTCAGCTGGTGGAGTCTGGCGGTGGCCTGGTGCAGCCA
GGGGGCTCACTCCGTTTGTCCTGTGCAGCTTCTGGCTTCAACCTCTCTTCTTATTCTATG
CACTGGGTGCGTCAGGCCCCGGGTAAGGGCCTGGAATGGGTTGCATCTATTTCTTCTTAT
TATGGCTATACTTCTTATGCCGATAGCGTCAAGGGCCGTTTCACTATAAGCGCAGACACA
TCCAAAAACACAGCCTACCTACAAATGAACAGCTTAAGAGCTGAGGACACTGCCGTCTAT
TATTGTGCTCGCTACGGTGTTTGGTACGGTGCTGGTATTGACTACTGGGGTCAAGGAACC
CTGGTCACCGTCTCCTCGGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCTCC
TCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC
GAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCG
GCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGC
AGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTC
GACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACTAA
ATGAAATACCTATTGCCTACGGCAGCCGCTGGCTTGCTGCTGCTGGCGGCCCAGCCGGCC
ATGGCGTCCGATATCCAGATGACCCAGTCCCCGAGCTCCCTGTCCGCCTCTGTGGGCGAT
AGGGTCACCATCACCTGCCGTGCCAGTCAGTCCGTGTCCAGCGCTGTAGCCTGGTATCAA
CAGAAACCAGGAAAAGCTCCGAAGCTTCTGATTTACTCGGCATCCAGCCTCTACTCTGGA
GTCCCTTCTCGCTTCTCTGGTAGCCGTTCCGGGACGGATTTCACTCTGACCATCAGCAGT
CTGCAGCCGGAAGACTTCGCAACTTATTACTGTCAGGGTTACTGGTGGGCTTACCCGATC
TTCGGACAGGGTACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC
TTCCCGCCATCTGATTCACAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT
AACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGT
AACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGC
ACCCTGACGCTGAGCAAAGCAGACTACGAAAAACATAAAGTCTACGCCTGCGAAGTCACC
CATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTGGTGGTTCT
GATTACAAAGATGACGATGACAAATAA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Mutations: None |
Discrepancy: None |
| 5' Linker Sequence: None | |
| 3' Linker Sequence: None | |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
| Protein Expression Results: |
ProteinExpressed: Protein_Expressed ProteinPurified: Protein_Purified SolubleProtein: Protein_Soluble |
| Recommended expression in: | Not Applicable |
Powered by LabGenius |
||
| Vector Name: | pBL166 | |
| Synonyms: | '' | |
| Description: | Recombinant antibody bacterial expression vector with C-terminal flag tagged light chain; ampicillin resistance in bacteria. | |
| Comments: | None | |
| Size (bp): | 7287 | |
| Parent Vector: | None | |
| Empty Vector: | None | |
| Properties: | bacterial expression, with tag/fusion/marker | |
| Author Name: |
Recombinant Antibody Network |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | pBR322 origin | pBR322 origin | 1224 | 1843 |
| gene fragment | ROP | ROP ORF | 2258 | 2449 |
| gene fragment | light chain | Light Chain with N-terminal PelB signal sequence | 5317 | 5382 |
| gene fragment | heavy chain | Heavy chain | 6179 | 6958 |
| repressor binding site | lac operator | lac operator | 5248 | 5275 |
| repressor protein gene | LacI | LacI | 3761 | 4852 |
| selectable marker | AmpR | ampicillin resistance | 209 | 1069 |
| tag | Flag | C-terminal Flag tag of the light chain | 6037 | 6060 |
| trxn termination sequence | T7 term | T7 transcription terminator | 6972 | 7100 |
- Protein
- Clone
Protein
| PCRP Binder Details : | anti-TBX5-RAB-S32 |
|---|
Clone
| Cloning Information : | 664612 |
|---|---|
| Original Clone ID : | anti-TBX5-RAB-S32 |
