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pntA (V. cholerae O1 biovar El Tor str. N16961 ) in pANT7_cGST

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Explanation of Terms


Gene: Gene Symbol:  pntA
Gene Name:  NAD(P) transhydrogenase, alpha subunit
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH219331.01F
Keyword: None
Species: Vibrio cholerae O1 biovar El Tor str. N16961
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
VcCD00061290

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1539nts         Open reading frame : 1 to 1539

ATGCAAATTGGTGTACCAAGAGAACAACTCGCTGGTGAAACACGAGTCGCTGCTTCTCCC
TCATCGGTAGAACAGCTTATTAAATTAGGATTTGAGGTTTGTATCGAATCAAAAGCCGGA
GCGCTTGCCAGTTTCGATGATGCCGCTTATACCGCAGCCGGCGCTACGATCGGCTCACGT
GAAGAGATTTGGGCTTGCCCACTTATCCTCAAAGTCAATGCTCCTTCAGATGACGAAATT
GCTCTTCTTAAGGAAGGCGCGACACTCGTAAGTTTTATTTGGCCAGCCCAAAATCCTGCT
TTGATGGAAAAACTCTCAAGTAAAAACATCAACGTACTGGCGATGGATGCGGTGCCTCGT
ATCTCTCGTGCGCAAGCATTGGATGCACTCTCGTCGATGGCGAACATTGCGGGTTACCGT
GCAGTGGTAGAAGCCGCTCATGAGTTTGGTCGTTTCTTTACTGGTCAAATTACTGCAGCA
GGTAAAGTATCGCCCGCGAAAGTCTTTGTTGCTGGTGCCGGCGTTGCTGGCTTAGCGGCA
ATAGGGGCTGCGGGTAGTTTAGGCGCTATCGTCCGTGCGTTTGACGTTCGTCCCGAAGTA
AAAGAGCAAGTGCAGTCTATGGGGGCTGAGTTTTTGGAAGTTAACTTCCAAGAGACTGCT
GGCTCAGGCGATGGTTATGCTAAAGAGATGTCGGATGAATTCAACCGTAAAGCGGCTGAA
CTTTACGCCGCTCAAGCGAAAGATGTCGATATCATTATTACTACCGCGCTGATTCCAGGA
AAACCTGCACCAAAGCTTATCACCAAAGAGATGGTAGATAGCATGAAAGCGGGCAGTGTG
ATTGTCGATCTGGCGGCAGCGAATGGCGGCAACTGTGAATACACAGTCAAAGATCAGGTT
ATTACCACAGACAACGGCGTTAAAGTGATCGGTTATACCGATATGGTAGGGCGTCTGCCG
ACCCAATCATCGCAACTGTATGCAACAAACCTAGTTAACTTGCTAAAACTACTCTGCAAA
GAAAAAGACGGCAATATCGACATCAACTTCGAAGATGTTGTGCTGCGTGGCGTCACCGTA
GTTAAAGCCGGCGAGATTACTTGGCCAGCGCCGCCTATTCAGGTATCCGCACAGCCTCAG
CAAAAAGCCAAAGTGCAACCGACTAAAGCGCAGAAGAAAGAGCCAGAGCCGACTTCTCCT
GTTAAAAAACTGGTTGGTTTAGCCGTGGGTGTCGGTCTGTTTGCGTGGGTAGCTTCTGTC
GCACCAGCCGCATTCTTAGGACACTTTACTGTGTTTGTCCTGGCCTGCGTCGTGGGTTAC
TACGTGGTATGGAATGTAACTCATGCTCTTCACACACCTTTAATGTCAGTGACTAACGCG
ATTTCTGGCATCATTGTAGTAGGGGCATTATTACAAATTGGTCAAGGCAGCGGTATTGTG
ACTTTCTTGGCTTTCATCGCCGTCTTAATTGCAAGCATCAACATCTTTGGTGGCTTTACC
GTTACCAAACGTATGCTTGAAATGTTCCGTAAAAACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1539
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Reagents

Gene

Target GenBank: NP_232953

 

Clone

Cloning Information : 219331
HIP Master Clone ID : 180750
Original Clone ID : FLH219331.01F

Organism


Species Specific ID: VCA0563

Reagents

Labome : pntA-cDNA