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Detailed Vector Information: pEnt3

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pEnt3
Type: No insert
Vector Name: pEnt3              
Source: Mekalanos lab
Description: Vector for transposon-mediated mutagenesis in bacteria, with Tn-magellan1 element; ampicillin and kanamycin resistance (kanamycin for selection of transposon insertions); restriction enzyme cloning.
Comments: None
Publications: PMID: 10500193
Title: Hyperactive transposase mutants of the Himar1 mariner transposon
PMID: 9671781
Title: Systematic identification of essential genes by in vitro mariner mutagenesis
Authors: John Mekalanos
Su Chiang
EvNO00061622

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AVAILABLE
CPD only
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pEnt3

pEnt3 in advanced viewed

Synonyms: None
Description: Vector for transposon-mediated mutagenesis in bacteria, with Tn-magellan1 element; ampicillin and kanamycin resistance (kanamycin for selection of transposon insertions); restriction enzyme cloning.
Comments: None
Size (bp): 0
Parent Vector: None
Properties: transposon mediated gene disruption
Author Name: John Mekalanos
Publications: PMID: 10500193
Title: Hyperactive transposase mutants of the Himar1 mariner transposon

PMID: 9671781
Title: Systematic identification of essential genes by in vitro mariner mutagenesis

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori E. coli origin of replication 0 0
selectable marker Tn-magellan1 transposon with kanR gene for selection of insertions 0 0
selectable marker AmpR ampicillin resistance gene (inferred) 0 0
transposon fragment Tn-magellan1 transposon with kanR gene for selection of insertions 0 0

 

Original Clone ID: pEnt3
SYNONYM: pEnt3 in DH5-alpha
SYNONYM: Mekalanos Strain C37
Species Specific ID: None
Target GenBank: None