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Detailed Vector Information: pFD1

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pFD1
Type: No insert
Vector Name: pFD1              
Source: Mekalanos lab
Description: Mariner transposon and transpoase-containing suicide vector for transposon-mediated mutagenesis in bacteria; amp and kanamycin resistance; restriction enzyme cloning.
Comments: The pFD1 plasmid is extremely unstable. Because of this, we suggest using multi-colony (rather than single-colony) techniques for manipulating and propagating the plasmid.Plasmid strain too unstable to verify and distribute. Contact Brian Ackerly at UMass for more information.
Publications: PMID: 9990078
Title: In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria
Authors: John Mekalanos
Su Chiang
EvNO00061623

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NOT AVAILABLE
CPD only
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : SM10 lambda-pir
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pFD1

pFD1 in advanced viewed

Synonyms: None
Description: "Suicide" vector for transposon-mediated mutagenesis in bacteria; amp and kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 0
Parent Vector: None
Properties: transposon mediated gene disruption
Author Name: John Mekalanos
Publications: PMID: 9990078
Title: In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria

Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication (inferred) 0 0
selectable marker AmpR ampicillin resistance gene (inferred) 0 0
selectable marker KanR kanamycin resistance gene (inferred) 0 0
transposon fragment transposon Mariner transposon 0 0
transposon fragment transposase Mariner transposase 0 0

 

Original Clone ID: pFD1
SYNONYM: pFD1 in SM10 lambda-pir
SYNONYM: Mekalanos Strain C34
Species Specific ID: None
Target GenBank: None