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Detailed Vector Information: pMEnt

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pMEnt
Type: No insert
Vector Name: pMEnt              
Source: Mekalanos lab
Description: Mariner transposon fragment-containing vector used to create pFD1; chloramphenicol, kanamycin and tetracycline resistance; restriction enzyme cloning.
Comments: None
Publications: PMID: 9990078
Title: In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria
Authors: John Mekalanos
Su Chiang
EvNO00061628

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AVAILABLE
CPD only
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : XL1 Blue
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  30 ug/mL chloramphenicol
Growth Condition:  Growth at 37 degrees C in LB supplemented with chloramphenicol (30 ug/mL)
Comments:  None
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Vector Name: pMEnt

pMEnt in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Mariner transposon fragment-containing vector used to create pFD1; chloramphenicol, kanamycin and tetracycline resistance; restriction enzyme cloning.
Comments: See also pFD1
Size (bp): 0
Parent Vector: None
Properties: transposon mediated gene disruption
Author Name: John Mekalanos
Publications: PMID: 9990078
Title: In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria

Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori E. coli origin of replication (inferred) 0 0
promoter lac pr lac promoter (drives transposase) 0 0
selectable marker TetR tetracycline resistance gene (inferred) 0 0
selectable marker CmR chloramphenicol resistance gene (inferred) 0 0
selectable marker KanR kanamycin resistance gene 0 0
transposon fragment transposase Himar1-type transposase 0 0
transposon fragment transposon minitransposon magellan3 with Himar1-type transposon repeats (flanks kanR) 0 0

 

Original Clone ID: pMEnt
SYNONYM: pMEnt in XL1Blue
SYNONYM: Mekalanos Strain C36
Species Specific ID: None
Target GenBank: None