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Detailed Vector Information: pMycoMar

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pMycoMar
Type: No insert
Vector Name: pMycoMar              
Source: Mekalanos lab
Description: Vector for transposon-mediated mutagenesis in mycobacteria, with ts origin of replication, mariner transposase and transposon, mycobacterial promoter for selectable marker; gentamycin, kanamycin, tetracycline resistance (kanamycin for selection of insertions in mycobacteria); restriction enzyme cloning.
Comments: None
Publications: PMID: 9990078
Title: In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria
Authors: John Mekalanos
Su Chiang
EvNO00061629

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AVAILABLE
CPD only
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : XL1 Blue
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mycobacteria    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
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Vector Name: pMycoMar

pMycoMar in advanced viewed

Synonyms: None
Description: Vector for transposon-mediated mutagenesis in mycobacteria, with ts origin of replication, mariner transposase and transposon, mycobacterial promoter for selectable marker; gentamycin, kanamycin, tetracycline resistance (kanamycin for selection of insertions in mycobacteria); restriction enzyme cloning.
Comments: None
Size (bp): 0
Parent Vector: None
Properties: transposon mediated gene disruption
Author Name: John Mekalanos
Publications: PMID: 9990078
Title: In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria

Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori E. coli origin of replication (inferred) 0 0
promoter myco mycobacterial promoter (driver for selection of transposon inserts in mycobacteria) 0 0
selectable marker TetR tetracycline resistance gene (inferred) 0 0
selectable marker GenR gentamicin resistance gene (inferred) 0 0
selectable marker KanR kanamycin resistance gene (inferred) 0 0
transposon fragment transpoase transposase source 0 0
transposon fragment transposon transposon elements (with selectable marker) 0 0

 

Original Clone ID: pMycoMar
SYNONYM: pMycoMar in XL1Blue
SYNONYM: Mekalanos Strain C35
Species Specific ID: None
Target GenBank: None