DNASU Plasmid Repository • 480.965.5697 | Email

NT5C2 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  NT5C2
Gene Name:  5'-nucleotidase, cytosolic II
Sequence             
Original Clone ID: HsCD00078337
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00616403

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1683nts         Open reading frame : 145 to 1827

GATTGTTGCCGTGCGCTGGAGCCGAGTTTCGCTCTGCTGCCCAGGCTGGAGTACAGTGAT
GTGATCTCCTGACGTCAAGTGATCCATTCGCCTCGGCCTCCCAAAGTTCTGGGATTACAA
GTAATACAAAATACAGTTAATAAAATGTCAACCTCCTGGAGTGATCGGTTACAGAATGCA
GCAGATATGCCTGCTAACATGGATAAGCATGCCCTGAAAAAGTATCGTCGAGAAGCCTAT
CATCGGGTGTTTGTGAACCGAAGTTTAGCAATGGAAAAGATAAAGTGTTTTGGTTTTGAT
ATGGATTATACCCTTGCTGTGTACAAGTCCCCAGAGTATGAGTCCCTTGGTTTTGAGCTT
ACTGTGGAGAGATTAGTTTCTATTGGCTATCCCCAGGAGTTGCTCAGCTTTGCTTATGAT
TCTACATTCCCTACCAGGGGACTTGTCTTTGACACACTGTATGGAAATCTTTTGAAAGTC
GATGCCTATGGAAACCTCTTGGTCTGTGCACATGGATTTAACTTTATAAGGGGACCAGAA
ACTAGAGAACAGTATCCAAATAAATTTATCCAGCGAGATGATACTGAAAGATTTTACATT
CTGAACACACTATTCAACCTACCAGAGACCTACCTGTTGGCCTGCCTAGTAGATTTTTTT
ACTAATTGTCCCAGATATACCAGTTGTGAAACAGGATTTAAAGATGGGGACCTCTTCATG
TCCTACCGGAGTATGTTCCAGGATGTAAGAGATGCTGTTGACTGGGTTCATTACAAGGGC
TCCCTTAAGGAAAAGACAGTTGAAAATCTTGAGAAGTATGTAGTCAAAGATGGAAAACTG
CCTTTGCTTCTGAGCCGGATGAAGGAAGTAGGGAAAGTATTTCTTGCTACCAACAGTGAC
TATAAATATACAGATAAAATTATGACTTACCTGTTTGACTTCCCACATGGCCCCAAGCCT
GGGAGCTCCCATCGACCATGGCAGTCCTACTTTGACTTGATCTTGGTGGATGCACGGAAA
CCACTCTTTTTTGGAGAAGGCACAGTACTGCGTCAGGTGGATACTAAAACTGGCAAGCTG
AAAATTGGTACCTACACAGGGCCCCTACAGCATGGTATCGTCTACTCAGGAGGTTCTTCT
GATACGATCTGTGACCTGTTGGGAGCCAAGGGAAAAGACATTTTGTATATTGGAGATCAC
ATTTTTGGGGACATTTTAAAATCAAAGAAACGGCAAGGGTGGCGAACTTTTTTGGTGATT
CCTGAACTCGCACAGGAGCTACATGTCTGGACTGACAAGAGTTCACTTTTCGAAGAACTT
CAGAGCTTGGATATTTTCTTGGCTGAACTCTACAAGCATCTTGACAGCAGTAGCAATGAG
CGTCCAGACATCAGTTCCATCCAGAGACGTATTAAGAAAGTAACTCATGACATGGACATG
TGCTATGGGATGATGGGAAGCCTGTTTCGCAGTGGCTCCCGGCAGACCCTTTTTGCCAGT
CAAGTGATGCGTTATGCTGACCTCTATGCAGCATCTTTCATCAACCTGCTGTATTACCCT
TTCAGCTACCTCTTCAGGGCTGCCCATGTCTTGATGCCTCATGAATCAACGGTGGAGCAC
ACACACGTAGATATCAATGAGATGGAGTCTCCTCTTGCCACCCGGAACCGCACATCAGTG
GATTTCAAAGACACTGACTACAAGCGGCACCAGCTGACACGGTCAATTAGTGAGATTAAA
CCTCCCAACCTCTTCCCACTGGCCCCCCAGGAAATTACACACTGCCATGACGAAGATGAT
GATGAAGAGGAGGAGGAGGAGGAAGAATAAGGAGGAAAACCAAAACCCCAAGCACCCATT
AAACAAGTCCTGGCAGGACTCACAGGAACAAACGAGGTCCCTGTTAGGGTTCTACTCGGG
GGAGGGAGGGGGCTCCATGAAAGGTACGTCTGAAAAGTTTCTGAAGATTTTATTATCATA
GATACTTGTTTTGGTTTTGTGTATCTGTACTCTCTGCAGATGGTCCAAAATTGTAATGGA
GTCTGTATTAGAAGAAAATAAGGGTAAAATCAGGCTGAACTGCATGTATATGGCTCCACT
GTGGCTTGTGACACTTTTAAAATCATCCGTATGTCAGTGTATCTGGATACACGAGGAAAA
GGAAAGAGTCTCAGAGTGGAACAAAGAGTGGGAAGAGGTGATCTGTAATGTTACAAATTG
TGCTATTACTCCAAGGTCCAACTTTTCCAGTGCATTACATGGTATTGTATATCAGTGGAG
AAATGTATTATTTCCATGATCAAATGTAGTCTCTGTTAAGGTCAAGTTTTCTTTTATAAG
CCTTTAATTCATCCTCAGTGACTCTGGCAAGGCTGCTTCTCTATCACTGGCTTTGCACAG
AAGTATGCTCTACTTGCGTTGCTTTAGGGCAGGATTCTATTTTGAGGGAAAAGACAGTAT
CCTTATTACCTTTTGTTTGTTTAATAGCACAAATGCTTATTTGTTATCCAAAAACAACCT
CCTTCTTATCTGTGATAAATCTATAGAAAGAATTTAGCTGCAAGTGGACAAAGGAACAAG
CCCCCAGAAAAGAAAGGGAAGAACTGCCTTCTTATACTACAGAACATGCATTAGTGTGGG
CTATATAGCTGTGGCTCATGCTACCCAATTCCAGATTTCTTTGTCCTCTAAGAGTTGATT
GCTGTATATTAAAATTGAACATCAGAGGATGGGAAGAGGGCTCTGTAAGCCAGAACCTTA
CTAAAGTAGAGGGCACAATCAGTGTGAATAAATTCACTTCAGAATCTCAAGTCAAGGCCA
GGCACGGCGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGACAGGCGGATCACC
TGAGGTCGGGAGTTCGAGACCAGCCTTACCAACATGGAGAAACCCCATCTCTACTAAAAA
TACAAAATTACCTGGGCGTGGTGGTGCATGCCTGTAATCCCATCATCTACTCAGGAGGCT
GAGGCAGGAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCCAGGATTGTGCCA
TTGCACTCCAGCCTGGGCAACAAGAACAAAACTCCATCTCAAAAAATAAAAATCCCAATC
CCAAGTCGAAATCACCTCTTGTTTTAAACAAGAATGAATCATTACTGTGTATGTTAGGGT
ATTAAAACTGTTTCACCAGTACAGTGAAAGTTGTTTCAACATTTTAAACAAACAGTGGTT
ATAGACTCTTTCTTTAACCATTGTATATTTTCTTCCATTCTTGTCATTGGTCAATAGGGG
AGGGTAGATTAGCTGCTCCAGAATTCAATAAAGTGTAATATTTCTAAAAAAAAAAAAAAA
AAAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NT5C2
Symbol Nomenclature : NT5C2
SYNONYM : GMP; NT5B; PNT5; cN-II
Designation : 5'-nucleotidase (purine), cytosolic type B|IMP-specific 5'-NT|cytosolic 5'-nucleotidase II|cytosolic purine 5'-nucleotidase|purine 5' nucleotidase
Full Nomenclature : 5'-nucleotidase, cytosolic II
GENEID : 22978
Locus Tag : RP11-30H12.3
HGNC : 8022
MIM : 600417
Vega : OTTHUMG00000018981
Target GenBank: BC001595

 

Pathways

Reactome : Abacavir metabolism
Reactome : Abacavir transport and metabolism
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Purine catabolism
Reactome : Purine metabolism

Protein

UniProt : A8K6K2
UniProt : P49902
HPRD : 02686

Clone

Cloning Information : 653964
HIP Master Clone ID : 162117
IMAGE ID : 3543103
Original Clone ID : FLH225269.01L
Original Clone ID : HsCD00078337

Organism


TAX_ID : 9606
Species Specific ID: 22978

Genome

Chromosome : 10
Map Location : 10q24.32
Ensembl : ENSG00000076685

Reagents

Labome : NT5C2-antibody