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GP5 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GP5
Gene Name:  glycoprotein V (platelet)
Sequence             
Original Clone ID: FLH469814.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00616701

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1680nts         Open reading frame : 32 to 1711

AGTTACTTTGGAGTGCAGAACCATTTCAGACATGCTGAGGGGGACTCTACTGTGCGCGGT
GCTCGGGCTTCTGCGCGCCCAGCCCTTCCCCTGTCCGCCAGCTTGCAAGTGTGTCTTCCG
GGACGCCGCGCAGTGCTCGGGGGGCGACGTGGCGCGCATCTCCGCGCTAGGCCTGCCCAC
CAACCTCACGCACATCCTGCTCTTCGGAATGGGCCGCGGCGTCCTGCAGAGCCAGAGCTT
CAGCGGCATGACCGTCCTGCAGCGCCTCATGATCTCCGACAGCCACATTTCCGCCGTTGC
CCCCGGCACCTTCAGTGACCTGATAAAACTGAAAACCCTGAGGCTGTCGCGCAACAAAAT
CACGCATCTTCCAGGTGCGCTGCTGGATAAGATGGTGCTCCTGGAGCAGTTGTTTTTGGA
CCACAATGCGCTAAGGGGCATTGACCAAAACATGTTTCAGAAACTGGTTAACCTGCAGGA
GCTCGCTCTGAACCAGAATCAGCTCGATTTCCTTCCTGCCAGTCTCTTCACGAATCTGGA
GAACCTGAAGTTGTTGGATTTATCGGGAAACAACCTGACCCACCTGCCCAAGGGGTTGCT
TGGAGCACAGGCTAAGCTCGAGAGACTTCTGCTCCACTCGAACCGCCTTGTGTCTCTGGA
TTCGGGGCTGTTGAACAGCCTGGGCGCCCTGACGGAGCTGCAGTTCCACCGAAATCACAT
CCGTTCCATCGCACCCGGGGCCTTCGACCGGCTCCCAAACCTCAGTTCTTTGACGCTTTC
GAGAAACCACCTTGCGTTTCTCCCCTCTGCGCTCTTTCTTCATTCGCACAATCTGACTCT
GTTGACTCTGTTCGAGAACCCGCTGGCAGAGCTCCCGGGGGTGCTCTTCGGGGAGATGGG
GGGCCTGCAGGAGCTGTGGCTGAACCGCACCCAGCTGCGCACCCTGCCCGCCGCCGCCTT
CCGCAACCTGAGCCGCCTGCGGTACTTAGGGGTGACTCTGAGCCCGCGGCTGAGCGCGCT
TCCGCAGGGCGCCTTCCAGGGCCTTGGCGAGCTCCAGGTGCTCGCCCTGCACTCCAACGG
CCTGACCGCCCTCCCCGACGGCTTGCTGCGCGGCCTCGGCAAGCTGCGCCAGGTGTCCCT
GCGCCGCAACAGGCTGCGCGCCCTGCCCCGTGCCCTCTTCCGCAATCTCAGCAGCCTGGA
GAGCGTCCAGCTCGACCACAACCAGCTGGAGACCCTGCCTGGCGACGTGTTTGGGGCTCT
GCCCCGGCTGACGGAGGTCCTGTTGGGGCACAACTCCTGGCGCTGCGACTGTGGCCTGGG
GCCCTTCCTGGGGTGGCTGCGGCAGCACCTAGGCCTCGTGGGCGGGGAAGAGCCCCCACG
GTGCGCAGGCCCTGGGGCGCACGCCGGCCTGCCGCTCTGGGCCCTGCCGGGGGGTGACGC
GGAGTGCCCGGGCCCCCGGGGCCCGCCTCCCCGCCCCGCTGCGGACAGCTCCTCGGAAGC
CCCTGTCCACCCAGCCTTGGCTCCCAACAGCTCAGAACCCTGGGTGTGGGCCCAGCCGGT
GACCACGGGCAAAGGTCAAGATCATAGTCCGTTCTGGGGGTTTTATTTTCTGCTTTTAGC
TGTTCAGGCCATGATCACCGTGATCATCGTGTTTGCTATGATTAAAATTGGCCAACTCTT
TCGAAAATTAATCAGAGAGAGAGCCCTTGGGTAAACCAATGGGAAAATCTTCTAATTACT
TAGAACCTGACCAGATGTGGCTCGGAGGGGAATCCAGACCCGCTGCTGTCTTGCTCTCCC
TCCCCTCCCCACTCCTCCTCTCTTCTTCCTCTTCTCTCTCACTGCCACGCCTTCCTTTCC
CTCCTCCTCCCCCTCTCCGCTCTGTGCTCTTCATTCTCACAGGCCCGCAACCCCTCCTCT
CTGTGTCCCCCGCCCGTTCCTGGAAACTGAGCTTGACGTTTGTAAACTGTGGTTGCCTGC
CTTCCCCAGCTCCCACGCGGGTGTGCGCTGACACTGCCGGGGGCGCTGGACTGTGTTGGA
CCCATCCGTGCTCCGCTGTGCCTGGCTTGGCGTCTGGTGGAGAGAGGGGCCTCTTCAGTG
TCTACTGAGTAAGGGGACAGCTCCAGGCCGGGGCCTGTCTCCTGCACAGAGTAAGCCGGT
AAATGTTTGTGAAATCAATGCGTGGATAAAGGAACACATGCCATCCAAGTGATGATGGCT
TTTCCTGGAGGGAAAGGATAGGCTGTTGCTCTATCTAATTTTTTGTTTTTGTTTTTGGAC
AGTCTAGCTCTGTGGCCCAGGCTGGCGTGCAGTGGGCCGTCTCAGTTCACTGCAGCCTCC
GCCTCCCAGGTTCAAGTGATTCTCATGCCTCAGCGTTCTGAGTAGCTGGGATTAGAGGCG
TGTGCCACTACACCCGGCTAATTTTTGTACTTTTTAAAGTAGAGACGGGGCTTTGCCATA
TTGGCCTGGCTGATCTCAAACTCCTGGTCTTGAACTCCTGGCCACAAGTGATCTGCCCGC
CTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTAAGCCACTACACCTGGCCCTCTTCATC
GAATTTTATTTGAGAAGTAGAGCTCTTGCCATTTTTTCCCTTGCTCCATTTTTCTCACTT
TATGTCTCTCTGACCTATGGGCTACTTGGGAGAGCACTGGACTCCATTCATGCATGAGCA
TTTTCAGGATAAGCGACTTCTGTGAGGCTGAGAGAGGAAGAAAACACGGAGCCTTCCCTC
CAGGTGCCCAGTGTAGGTCCAGCGTGTTTCCTGAGCCTCCTGTGAGTTTCCACTTGCTTT
ACATCCATGCAACATGTCATTTTGAAACTGGATTGATTTGCATTTCCTGGAACTCTGCCA
CCTCATTTCACAAGCATTTATGGAGCAGTTAACATGTGACTGGTATTCATGAATATAATG
ATAAGCTTGATTCTAGTTCAGCTGCTGTCACAGTCTCATTTGTTCTTCCAACTGAAAGCC
GTAAAACCTTTGTTGCTTTAATTGAATGTCTGTGCTTATGAGAGGCAGTGGTTAAAACAG
GGGCTGGCGAGTTGACAACTGTGGGTTCAAATCCCAGCTCTACCACTTACTAACTGCATG
GGACTTTGGGTAAGACACCTGCTTACATTCTCTAAGCCTTGGTTTCCTGAACCTTAAAAC
AGGATAACATAGTACCTGCTTCGTAGAGTTTTTGTGAGAATTAAAGGCAATAAAGCATAT
AATGACTTAGCCCAGCGGCCTGCAGGCAATACATGTTAATGAATGTTAGCTATTATTACT
AAAGGATGAGCAATTATTATTGGCATCATGATTTCTAAAGAAGAGCTTTGAGTTGGTATT
TTTCTCTGTGTATAAGGGTAAGTCCGAACTTTCTCAGACTGGAGGTTACATTCACATCAG
TCTGTCTTCCCCTGCGGATGGCCTCAGCCCTGGGTGGCCAGACTCTGTGCTCACAATCCA
GAGCAATGGATCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GP5
Symbol Nomenclature : GP5
SYNONYM : CD42d; GPV
Designation : glycoprotein 5|platelet glycoprotein V
Full Nomenclature : glycoprotein V (platelet)
GENEID : 2814
HGNC : 4443
MIM : 173511
Target GenBank: NM_004488

 

Pathways

Panther : Blood coagulation
Reactome : Formation of Fibrin Clot (Clotting Cascade)
Reactome : GP1b-IX-V activation signalling
Reactome : Hemostasis
Reactome : Intrinsic Pathway
Reactome : Platelet Adhesion to exposed collagen
Reactome : Platelet Aggregation (Plug Formation)
Reactome : Platelet activation, signaling and aggregation

Clone

Cloning Information : 638499
HIP Master Clone ID : 201544
Original Clone ID : HsCD00302876
Original Clone ID : FLH469814.01L

Organism


TAX_ID : 9606
Species Specific ID: 2814

Genome

Chromosome : 3
Map Location : 3q29
Ensembl : ENSG00000178732

Reagents

Labome : GP5-antibody