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metX (Pseudomonas aeruginosa) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  metX
Gene Name:  homoserine O-acetyltransferase
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH037153.01F
Keyword: None
Species: Pseudomonas aeruginosa
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
PaCD00006169

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1140nts         Open reading frame : 1 to 1140

ATGCCCACAGTCTTCCCCGACGACTCCGTCGGTCTGGTCTCCCCCCAGACGCTGCACTTC
AACGAACCGCTCGAGCTGACCAGCGGCAAGTCCCTGGCCGAGTACGACCTGGTGATCGAA
ACCTACGGCGAGCTGAATGCCACGCAGGGCAACGCGGTGCTGATCTGCCACGCCCTCTCC
GGCCACCACCACGCCGCCGGCTACCACAGCGTCGACGAGCGCAAGCCGGGCTGGTGGGAC
AGCTGCATCGGTCCGGGCAAGCCGATCGACACCCGCAAGTTCTTCGTCGTCGCCCTCAAC
AACCTCGGCGGTTGCAACGGATCCAGCGGCCCCGCCAGCATCAATCCGGCGACCGGCAAG
GTCTACGGCGCGGACTTCCCGATGGTTACGGTGGAAGACTGGGTGCATAGCCAGGCGCGC
CTGGCAGACCGCCTCGGCATCCGCCAGTGGGCCGCGGTGGTCGGCGGCAGCCTCGGCGGC
ATGCAGGCGCTGCAATGGACCATCAGCTATCCCGAGCGCGTCCGTCACTGCCTGTGCATC
GCCAGCGCGCCGAAGCTGTCGGCGCAGAACATCGCCTTCAACGAAGTCGCCCGGCAGGCG
ATTCTTTCCGACCCTGAGTTCCTCGGCGGCTACTTCCAGGAGCAGGGCGTGATTCCCAAG
CGCGGCCTCAAGCTGGCGCGGATGGTCGGCCATATCACCTACCTGTCCGACGACGCCATG
GGCGCCAAGTTCGGCCGTGTACTGAAGACCGAGAAGCTCAACTACGACCTGCACAGCGTC
GAGTTCCAGGTCGAGAGTTACCTGCGCTACCAGGGCGAGGAGTTCTCCACCCGCTTCGAC
GCCAATACCTACCTGCTGATGACCAAGGCGCTGGACTACTTCGACCCCGCCGCCGCCCAC
GGCGACGACCTGGTGCGCACCCTGGAGGGCGTCGAGGCGGACTTCTGCCTGATGTCCTTC
ACCACCGACTGGCGTTTCTCGCCGGCCCGCTCGCGGGAAATCGTCGACGCCCTGATCGCG
GCGAAAAAGAACGTCAGCTACCTGGAGATCGACGCCCCGCAAGGCCACGACGCCTTCCTC
ATGCCGATCCCCCGGTACCTGCAAGCCTTCAGCGGTTACATGAACCGCATCAGCGTGGGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1140
Start on reference sequence 1
Mutations:
None
Discrepancy:
a148g,S50G
                                

5' Linker Sequence:
5p ACAAGTTTGTACAAAAAAGCAGGCTCCGAAGGAGATACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Reagents

Gene

 

Gene Symbol : metX
GENEID : 878549
Locus Tag : PA0390
GI : 49088813
GenBank Accession : AY659334
Pseudomonas aeruginosa (PA) number : PA0390
Target GenBank: NP_249081

 

Protein

UniProt : P57714

Clone

Cloning Information : 37153
HIP Master Clone ID : 19534
Original Clone ID : FLH037153.01F

Organism


TAX_ID : 208964
Species Specific ID: 878549

Reagents

Labome : metX-cDNA