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CKM (Homo sapiens) in pLDNT7_nFLAG (Flag-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CKM
Gene Name:  creatine kinase, muscle
Sequence             
Original Clone ID: FLH230885.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLDNT7_nFLAG               Format:  CLOSED
Source: Center for Personalized Diagnostics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00617994

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1146nts         Open reading frame : 74 to 1219

GGGTCAGTGTCACCTCCAGGATACAGACAGCCCCCCTTCAGCCCAGCCCAGCCAGGTCTC
CTACACCGCCACCATGCCATTCGGTAACACCCACAACAAGTTCAAGCTGAATTACAAGCC
TGAGGAGGAGTACCCCGACCTCAGCAAACATAACAACCACATGGCCAAGGTACTGACCCT
TGAACTCTACAAGAAGCTGCGGGACAAGGAGACTCCATCTGGCTTCACTGTAGACGATGT
CATCCAGACAGGAGTGGACAACCCAGGTCACCCCTTCATCATGACCGTGGGCTGCGTGGC
TGGTGATGAGGAGTCCTACGAAGTTTTCAAGGAACTCTTTGACCCCATCATCTCGGATCG
CCACGGGGGCTACAAACCCACTGACAAGCACAAGACTGACCTCAACCATGAAAACCTCAA
GGGTGGAGACGACCTGGACCCCAACTACGTGCTCAGCAGCCGCGTCCGCACTGGCCGCAG
CATCAAGGGCTACACGTTGCCCCCACACTGCTCCCGTGGCGAGCGCCGGGCGGTGGAGAA
GCTCTCTGTGGAAGCTCTCAACAGCCTGACGGGCGAGTTCAAAGGGAAGTACTACCCTCT
GAAGAGCATGACGGAGAAGGAGCAGCAGCAGCTCATCGATGACCACTTCCTGTTCGACAA
GCCCGTGTCCCCGCTGCTGCTGGCCTCAGGCATGGCCCGCGACTGGCCCGACGCCCGTGG
CATCTGGCACAATGACAACAAGAGCCTCCTGGTGTGGGTGAACGAGGAGGATCACCTCCG
GGTCATCTCCATGGAGAAGGGGGGCAACATGAAGGAGGTTTTCCGCCGCTTCTGCGTAGG
GCTGCAGAAGATTGAGGAGATCTTTAAGAAAGCTGGCCACCCCTTCATGTGGAACCAGCA
CCTGGGCTACGTGCTCACCTGCCCATCCAACCTGGGCACTGGGCTGCGTGGAGGCGTGCA
TGTGAAGCTGGCGCACCTGAGCAAGCACCCCAAGTTCGAGGAGATCCTCACCCGCCTGCG
TCTGCAGAAGAGGGGTACAGGTGGCGTGGACACAGCTGCCGTGGGCTCAGTATTTGACGT
GTCCAACGCTGATCGGCTGGGCTCGTCCGAAGTAGAACAGGTGCAGCTGGTGGTGGATGG
TGTGAAGCTCATGGTGGAAATGGAGAAGAAGTTGGAGAAAGGCCAGTCCATCGACGACAT
GATCCCCGCCCAGAAGTAGGCGCCTGCCCACCTGCCACCGACTGCTGGAACCCAGCCAGT
GGGAGGGCCTGGCCCACCAGAGTCCTGCTCCCTCACTCCTCGCCCCGCCCCCTGTCCCAG
AGTCCCACCTGGGGGCTCTCTCCACCCTTCTCAGAGTTCCAGTTTCAACCAGAGTTCCAA
CCAATGGGCTCCATCCTCTGGATTCTGGCCAATGAAATATCTCCCTGGCAGGGTCCTCTT
CTTTTCCCAGAGCTCCACCCCAACCAGGAGCTCTAGTTAATGGAGAGCTCCCAGCACACT
CGGAGCTTGTGCTTTGTCTCCACGCAAAGCGATAAATAAAAGCATTGGTGGCCTTAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLDNT7_nFLAG

pLDNT7_nFLAG in advanced viewed

Synonyms: pLDNT7-Flag
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments: None
Size (bp): 4557
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name: Daniel Schiwek
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ori ColE1 origin of replication 1842 2524
promoter T7 T7 promoter 4462 4479
recombination site LoxP LoxP site for recombinational cloning 657 690
selectable marker AmpR ampicillin resistance gene 2622 3281
ssDNA origin f1 ori f1 origin for ssDNA production 3928 4368
tag Flag 3xFlag epitope tag ORF and LoxP region 585 650
trxn termination sequence T7 term T7 terminator sequence 1614 1660

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CKM
Symbol Nomenclature : CKM
SYNONYM : CKMM; M-CK
Designation : creatine kinase M chain|creatine kinase M-type
Full Nomenclature : creatine kinase, muscle
GENEID : 1158
HGNC : 1994
MIM : 123310
Vega : OTTHUMG00000181782
Target GenBank: BC007462

 

Pathways

NCI : Regulation of retinoblastoma protein
Reactome : Creatine metabolism
Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
WikiPathway : Urea cycle and metabolism of amino groups

Protein

UniProt : B2R892
UniProt : P06732
HPRD : 00426

Clone

Cloning Information : 654715
HIP Master Clone ID : 87288
IMAGE ID : 3934232
Original Clone ID : FLH231910.01L
Original Clone ID : HsCD00299568
Original Clone ID : HsCD00298737
Original Clone ID : FLH230885.01X

Organism


TAX_ID : 9606
Species Specific ID: 1158

Genome

Chromosome : 19
Map Location : 19q13.32
Ensembl : ENSG00000104879

Reagents

Labome : CKM-antibody