DNASU Plasmid Repository • 480.965.5697 | Email

SRC (Homo sapiens) in pLDNT7_nFLAG (Flag-tagged in vitro expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  SRC
Gene Name:  v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
Sequence             
Original Clone ID: HsCD00298643
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLDNT7_nFLAG               Format:  CLOSED
Source: Center for Personalized Diagnostics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00618035

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1611nts         Open reading frame : 40 to 1650

CATCGAGGTTTTGAGAGGCTAACTCTCCCAAAAAGGACCATGGGTAGCAACAAGAGCAAG
CCCAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCGCCGAGAACGTGCACGGCGCT
GGCGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAGCCAGCCTCGGCCGACGGCCAC
CGCGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAGCCCAAGCTGTTCGGAGGCTTC
AACTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGCCCGCTGGCCGGTGGAGTGACC
ACCTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAGACAGACCTGTCCTTCAAGAAA
GGCGAGCGGCTCCAGATTGTCAACAACACAGAGGGAGACTGGTGGCTGGCCCACTCGCTC
AGCACAGGACAGACAGGCTACATCCCCAGCAACTACGTGGCGCCCTCCGACTCCATCCAG
GCTGAGGAGTGGTATTTTGGCAAGATCACCAGACGGGAGTCAGAGCGGTTACTGCTCAAT
GCAGAGAACCCGAGAGGGACCTTCCTCGTGCGAGAAAGTGAGACCACGAAAGGTGCCTAC
TGCCTCTCAGTGTCTGACTTCGACAACGCCAAGGGCCTCAACGTGAAGCACTACAAGATC
CGCAAGCTGGACAGCGGCGGCTTCTACATCACCTCCCGCACCCAGTTCAACAGCCTGCAG
CAGCTGGTGGCCTACTACTCCAAACACGCCGATGGCCTGTGCCACCGCCTCACCACCGTG
TGCCCCACGTCCAAGCCGCAGACTCAGGGCCTGGCCAAGGATGCCTGGGAGATCCCTCGG
GAGTCGCTGCGGCTGGAGGTCAAGCTGGGCCAGGGCTGCTTTGGCGAGGTGTGGATGGGG
ACCTGGAACGGTACCACCAGGGTGGCCATCAAAACCCTGAAGCCTGGCACGATGTCTCCA
GAGGCCTTCCTGCAGGAGGCCCAGGTCATGAAGAAGCTGAGGCATGAGAAGCTGGTGCAG
TTGTATGCTGTGGTTTCAGAGGAGCCCATTTACATCGTCACGGAGTACATGAGCAAGGGG
AGTTTGCTGGACTTTCTCAAGGGGGAGACAGGCAAGTACCTGCGGCTGCCTCAGCTGGTG
GACATGGCTGCTCAGATCGCCTCAGGCATGGCGTACGTGGAGCGGATGAACTACGTCCAC
CGGGACCTTCGTGCAGCCAACATCCTGGTGGGAGAGAACCTGGTGTGCAAAGTGGCCGAC
TTTGGGCTGGCTCGGCTCATTGAAGACAATGAGTACACGGCGCGGCAAGGTGCCAAATTC
CCCATCAAGTGGACGGCTCCAGAAGCTGCCCTCTATGGCCGCTTCACCATCAAGTCGGAC
GTGTGGTCCTTCGGGATCCTGCTGACTGAGCTCACCACAAAGGGACGGGTGCCCTACCCT
GGGATGGTGAACCGCGAGGTGCTGGACCAGGTGGAGCGGGGCTACCGGATGCCCTGCCCG
CCGGAGTGTCCCGAGTCCCTGCACGACCTCATGTGCCAGTGCTGGCGGAAGGAGCCTGAG
GAGCGGCCCACCTTCGAGTACCTGCAGGCCTTCCTGGAGGACTACTTCACGTCCACCGAG
CCCCAGTACCAGCCCGGGGAGAACCTCTAGGCACAGGCGGGCCCAGACCGGCTTCTCGGC
TTGGATCCTGGGCTGGGTGGCCCCTGTCTCGGGGCTTGCCCCACTCTGCCTGCCTGCTGT
TGGTCCTCTCTCTGTGGGGCTGAATTGCCAGGGGCGAGGCCCTTCCTCTTTGGTGGCATG
GAAGGGGCTTCTGGACCTAGGGTGGCCTGAGAGGGCGGTGGGTATGCGAGACCAGCACGG
TGACTCTGTCCAGCTCCCGCTGTGGCCGCACGCCTCTCCCTGCACTCCCTCCTGGAGCTC
TGTGGGTCTCTGGAAGAGGAACCAGGAGAAGGGCTGGGGCCGGGGCTGAGGGTGCCCTTT
TCCAGCCTCAGCCTACTCCGCTCACTGAACTCCTTCCCCACTTCTGTGCCACCCCCGGTC
TATGTCGAGAGCTGGCCAAAGAGCCTTTCCAAAGAGGAGCGATGGGCCCCTGGCCCCGCC
TGCCTGCCACCCTGCCCCTTGCCATCCATTCTGGAAACACCTGTAGGCAGAGGCTGCCGA
GACAGACCCTCTGCCGCTGCTTCCAGGCTGGGCAGCACAAGGCCTTGCCTGGCCTGATGA
TGGTGGGTGGGTGGGATGAGTACCCCCTCAAACCCTGCCCTCCTTAGACCTGAGGGACCC
TTCGAGATCATCACTTCCTTGCCCCCATTTCACCCATGGGGAGACAGTTGAGAGCGGGGA
TGTGACATGCCCAAGGCCACGGAGCAGTTCAGAGTGGAGGCGGGCTTGGAACCCGGTGCT
CCCTCTGTCATCCTCAGGAACCAACAATTCGTCGGAGGCATCATGGAAAGACTGGGACAG
CCCAGGAAACAAGGGGTCTGAGGATGCATTCGAGATGGCAGATTCCCACTGCCGCTGCCC
GCTCAGCCCAGCTGTTGGGAACAGCATGGAGGCAGATGTGGGGCTGAGCTGGGGAATCAG
GGTAAAAGGTGCAGGTGTGGAGAGAGAGGCTTCAATCGGCTTGTGGGTGATGTTTGACCT
TCAGAGCCAGCCGGCTATGAAAGGGAGCGAGCCCCTCGGCTCTGGAGGCAATCAAGCAGA
CATAGAAGAGCCAAGAGTCCAGGAGGCCCTGGTCCTGGCCTCCTTCCCCGTACTTTGTCC
CGTGGCATTTCAATTCCTGGCCCTGTTCTCCTCCCCAAGTCGGCACCCTTTAACTCATGA
GGAGGGAAAAGAGTGCCTAAGCGGGGGTGAAAGAGGACGTGTTACCCACTGCCATGCACC
AGGACTGGCTGTGTAACCTTGGGTGGCCCCTGCTGTCTCTCTGGGCTGCAGAGTCTGCCC
CACATGTGGCCATGGCCTCTGCAACTGCTCAGCTCTGGTCCAGGCCCTGTGGCAGGACAC
ACATGGTGAGCCTAGCCCTGGGACATCAGGAGACTGGGCTCTGGCTCTGTTCGGCCTTTG
GGTGTGTGGTGGATTCTCCCTGGGCCTCAGTGTGCCCATCTGTAAAGGGGCAGCTGACAG
TTTGTGGCATCTTGCCAAGGGTCCCTGTGTGTGTGTATGTGTGTGCATGTGTGCGTGTCT
CCATGTGCGTCCATATTTAACATGTAAAAATGTCCCCCCCGCTCCGTCCCCCAAACATGT
TGTACATTTCACCATGGCCCCCTCATCATAGCAATAACATTCCCACTGCCAGGGGTTCTT
GAGCCAGCCAGGCCCTGCCAGTGGGGAAGGAGGCCAAGCAGTGCCTGCCTATGAAATTTC
AACTTTTCCTTTCATACGTCTTTATTACCCAAGTCTTCTCCCGTCCATTCCAGTCAAATC
TGGGCTCACTCACCCCAGCGAGCTCTCAAATCCCTCTCCAACTGCCTAAGGCCCTTTGTG
TAAGGTGTCTTAATACTGTCCTTTTTTTTTTTTTAACAGTGTTTTGTAGATTTCAGATGA
CTATGCAGAGGCCTGGGGGACCCCTGGCTCTGGGCCGGGCCTGGGGCTCCGAAATTCCAA
GGCCCAGACTTGCGGGGGGTGGGGGGGTATCCAGAATTGGTTGTAAATACTTTGCATATT
GTCTGATTAAACACAAACAGACCTCAGAAAAAAAAAAAAAAAAAAAAAAAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLDNT7_nFLAG

pLDNT7_nFLAG in advanced viewed

Synonyms: pLDNT7-Flag
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments: None
Size (bp): 4557
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name: Daniel Schiwek
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ori ColE1 origin of replication 1842 2524
promoter T7 T7 promoter 4462 4479
recombination site LoxP LoxP site for recombinational cloning 657 690
selectable marker AmpR ampicillin resistance gene 2622 3281
ssDNA origin f1 ori f1 origin for ssDNA production 3928 4368
tag Flag 3xFlag epitope tag ORF and LoxP region 585 650
trxn termination sequence T7 term T7 terminator sequence 1614 1660

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRC
Symbol Nomenclature : SRC
SYNONYM : ASV; SRC1; c-SRC; p60-Src
Designation : proto-oncogene c-Src|proto-oncogene tyrosine-protein kinase Src|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
Full Nomenclature : v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
GENEID : 6714
Locus Tag : RP5-823N20.1
HGNC : 11283
MIM : 190090
Vega : OTTHUMG00000032417
Target GenBank: BC011566

 

Pathways

NCI : Alpha-synuclein signaling
NCI : Alpha4 beta1 integrin signaling events
NCI : Alpha9 beta1 integrin signaling events
NCI : Arf6 signaling events
NCI : Atypical NF-kappaB pathway
NCI : CDC42 signaling events
NCI : CXCR3-mediated signaling events
NCI : CXCR4-mediated signaling events
NCI : Class I PI3K signaling events
NCI : Class I PI3K signaling events mediated by Akt
NCI : E-cadherin signaling in keratinocytes
NCI : E-cadherin signaling in the nascent adherens junction
NCI : EGF receptor (ErbB1) signaling pathway
NCI : EPHA forward signaling
NCI : EPHA2 forward signaling
NCI : EPHB forward signaling
NCI : Endothelins
NCI : Ephrin B reverse signaling
NCI : ErbB1 downstream signaling
NCI : ErbB2/ErbB3 signaling events
NCI : FAS (CD95) signaling pathway
NCI : FGF signaling pathway
NCI : Glypican 1 network
NCI : Integrins in angiogenesis
NCI : Internalization of ErbB1
NCI : LPA receptor mediated events
NCI : Nectin adhesion pathway
NCI : Netrin-mediated signaling events
NCI : Nongenotropic Androgen signaling
NCI : PDGFR-beta signaling pathway
NCI : Plasma membrane estrogen receptor signaling
NCI : Posttranslational regulation of adherens junction stability and dissassembly
NCI : Regulation of Androgen receptor activity
NCI : Regulation of p38-alpha and p38-beta
NCI : S1P3 pathway
NCI : Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI : Signaling events mediated by PRL
NCI : Signaling events mediated by PTP1B
NCI : Signaling events mediated by TCPTP
NCI : Signaling events mediated by VEGFR1 and VEGFR2
NCI : Signaling events mediated by focal adhesion kinase
NCI : Signaling events regulated by Ret tyrosine kinase
NCI : Syndecan-2-mediated signaling events
NCI : Syndecan-3-mediated signaling events
NCI : Thromboxane A2 receptor signaling
NCI : Trk receptor signaling mediated by PI3K and PLC-gamma
NCI : Urokinase-type plasminogen activator (uPA) and uPAR-mediated signaling
NCI : amb2 Integrin signaling
Panther : Angiogenesis
Panther : Cadherin signaling pathway
Panther : Integrin signalling pathway
Panther : Parkinson disease
Reactome : ADP signalling through P2Y purinoceptor 1
Reactome : Adaptive Immune System
Reactome : Axon guidance
Reactome : CD28 co-stimulation
Reactome : CTLA4 inhibitory signaling
Reactome : Cell surface interactions at the vascular wall
Reactome : Cell-Cell communication
Reactome : Costimulation by the CD28 family
Reactome : DCC mediated attractive signaling
Reactome : Developmental Biology
Reactome : Disease
Reactome : Downstream signal transduction
Reactome : FCGR activation
Reactome : Fcgamma receptor (FCGR) dependent phagocytosis
Reactome : GAB1 signalosome
Reactome : GP1b-IX-V activation signalling
Reactome : GRB2:SOS provides linkage to MAPK signaling for Integrins
Reactome : Gap junction trafficking and regulation
Reactome : Hemostasis
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Integrin alphaIIb beta3 signaling
Reactome : L1CAM interactions
Reactome : Membrane Trafficking
Reactome : NCAM signaling for neurite out-growth
Reactome : NGF signalling via TRKA from the plasma membrane
Reactome : Negative regulation of FGFR signaling
Reactome : Netrin mediated repulsion signals
Reactome : Netrin-1 signaling
Reactome : PECAM1 interactions
Reactome : Platelet Aggregation (Plug Formation)
Reactome : Platelet activation, signaling and aggregation
Reactome : Recycling pathway of L1
Reactome : Regulation of KIT signaling
Reactome : Regulation of gap junction activity
Reactome : Signal Transduction
Reactome : Signal amplification
Reactome : Signal regulatory protein (SIRP) family interactions
Reactome : Signaling by EGFR
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by FGFR
Reactome : Signaling by FGFR in disease
Reactome : Signaling by PDGF
Reactome : Signaling by SCF-KIT
Reactome : Signalling by NGF
Reactome : Signalling to ERKs
Reactome : Signalling to RAS
Reactome : Spry regulation of FGF signaling
Reactome : Thrombin signalling through proteinase activated receptors (PARs)
Reactome : c-src mediated regulation of Cx43 function and closure of gap junctions
Reactome : p130Cas linkage to MAPK signaling for integrins
Reactome : p38MAPK events
WikiPathway : Alpha 6 Beta 4 signaling pathway
WikiPathway : Androgen receptor signaling pathway
WikiPathway : Angiogenesis
WikiPathway : EPO Receptor Signaling
WikiPathway : ErbB signaling pathway
WikiPathway : FSH signaling pathway
WikiPathway : Focal Adhesion
WikiPathway : IL-3 Signaling Pathway
WikiPathway : Integrin-mediated cell adhesion
WikiPathway : Kit receptor signaling pathway
WikiPathway : Leptin signaling pathway
WikiPathway : Notch Signaling Pathway
WikiPathway : Prolactin Signaling Pathway
WikiPathway : RANKL/RANK Signaling Pathway
WikiPathway : Senescence and Autophagy
WikiPathway : Signaling of Hepatocyte Growth Factor Receptor
WikiPathway : TGF beta Signaling Pathway
WikiPathway : TSH signaling pathway
WikiPathway : angiogenesis overview

Clone

Cloning Information : 654808
HIP Master Clone ID : 111566
Original Clone ID : FLH231598.01L
Original Clone ID : HsCD00299282
Original Clone ID : FLH230778.01L
Original Clone ID : HsCD00298643

Organism


TAX_ID : 9606
Species Specific ID: 6714

Genome

Chromosome : 20
Map Location : 20q12-q13
Ensembl : ENSG00000197122

Reagents

Labome : Src-antibody