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TK1 (Homo sapiens) in pLDNT7_nFLAG (Flag-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TK1
Gene Name:  thymidine kinase 1, soluble
Sequence             
Original Clone ID: FLH230883.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLDNT7_nFLAG               Format:  CLOSED
Source: Center for Personalized Diagnostics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00618069

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 705nts         Open reading frame : 37 to 741

GGCACGAGGCGGGTTCGTGAACTTCCCGGAGGCGCAATGAGCTGCATTAACCTGCCCACT
GTGCTGCCCGGCTCCCCCAGCAAGACCCGGGGGCAGATCCAGGTGATTCTCGGGCCGATG
TTCTCAGGAAAAAGCACAGAGTTGATGAGACGCGTCCGTCGCTTCCAGATTGCTCAGTAC
AAGTGCCTGGTGATCAAGTATGCCAAAGACACTCGCTACAGCAGCAGCTTCTGCACACAT
GACCGGAACACCATGGAGGCGCTGCCCGCCTGCCTGCTCCGAGACGTGGCCCAGGAGGCC
CTGGGCGTGGCTGTCATAGGCATCGACGAGGGGCAGTTTTTCCCTGACATCGTGGAGTTC
TGCGAGGCCATGGCCAACGCCGGGAAGACCGTAATTGTGGCTGCACTGGATGGGACCTTC
CAGAGGAAGCCATTTGGGGCCATCCTGAACCTGGTGCCGCTGGCCGAGAGCGTGGTGAAG
CTGACGGCGGTGTGCATGGAGTGCTTCCGGGAAGCCGCCTATACCAAGAGGCTCGGCACA
GAGAAGGAGGTCGAGGTGATTGGGGGAGCAGACAAGTACCACTCCGTGTGTCGGCTCTGC
TACTTCAAGAAGGCCTCAGGCCAGCCTGCCGGGCCGGACAACAAAGAGAACTGCCCAGTG
CCAGGAAAGCCAGGGGAAGCCGTGGCTGCCAGGAAGCTCTTTGCCCCACAGCAGATTCTG
CAATGCAGCCCTGCCAACTGAGGGACCTGCGAGGGCCGCCCGCTCCCTTCCTGCCACTGC
CGCCTACTGGACGCTGCCCTGCATGCTGCCCAGCCACTCCAGGAGGAAGTCGGGAGGCGT
GGAGGATGACCACACCTTGGCCTTCTGGGAACTCTCCTTTGTGTGGCTGCCCCACCTGCC
GCATGCTCCCTCCTCTCCTACCCACTGGTCTGCTTAAAGCTTCCCTCTCAGCTGCTGGGA
CGATCGCCCAGGCTGGAGCTGGCCCCGCTTGGTGGCCTGGGATCTGGCACACTCCCTCTC
CTTGGGGTGAGGGACAGAGCCCCACGCTGTTGACATCAGCCTGCTTCTTCCCCTCTGCGG
CTTTCACTGCTGAGTTTCTGTTCTCCCTGGGAAGCCTGTGCCAGCACTTTTGAGCCTTGG
CCCACACTGAGGCTTAGGCCTCTCTGCCTGGGATGGGCTCCCACCCTCCCCTGAGGATGG
CCTGGATTCACGCCCTCTTGTTTCCTTTTGGGCTCAAAGCCCTTCCTACCTCTGGTGATG
GTTTCCACAGGAACAACAGCATCTTTCACCAAGATGGGTGGCACCAACCTTGCTGGGACT
TGGATCCCAGGGGCTTATCTCTTCAAGTGTGGAGAGGGCAGGGTCCACGCCTCTGCTGTA
GCTTATGAAATTAACTAATTGAAAATTCAAAAAAAAAAAAAAAAAAAAAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLDNT7_nFLAG

pLDNT7_nFLAG in advanced viewed

Synonyms: pLDNT7-Flag
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments: None
Size (bp): 4557
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name: Daniel Schiwek
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ori ColE1 origin of replication 1842 2524
promoter T7 T7 promoter 4462 4479
recombination site LoxP LoxP site for recombinational cloning 657 690
selectable marker AmpR ampicillin resistance gene 2622 3281
ssDNA origin f1 ori f1 origin for ssDNA production 3928 4368
tag Flag 3xFlag epitope tag ORF and LoxP region 585 650
trxn termination sequence T7 term T7 terminator sequence 1614 1660

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TK1
Symbol Nomenclature : TK1
SYNONYM : TK2
Designation : thymidine kinase 1 soluble isoform|thymidine kinase, cytosolic|thymidine kinase-1
Full Nomenclature : thymidine kinase 1, soluble
GENEID : 7083
HGNC : 11830
MIM : 188300
Vega : OTTHUMG00000150674
Target GenBank: BC007872

 

Pathways

NCI : E2F transcription factor network
NCI : Validated targets of C-MYC transcriptional activation
Panther : Salvage pyrimidine deoxyribonucleotides
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Pyrimidine metabolism
Reactome : Pyrimidine salvage reactions
WikiPathway : Fluoropyrimidine Activity

Protein

UniProt : P04183
HPRD : 01771

Clone

Cloning Information : 654913
HIP Master Clone ID : 54395
IMAGE ID : 4303880
Original Clone ID : HsCD00298735
Original Clone ID : FLH230883.01X

Organism


TAX_ID : 9606
Species Specific ID: 7083

Genome

Chromosome : 17
Map Location : 17q23.2-q25.3
Ensembl : ENSG00000167900

Reagents

Labome : TK1-antibody